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    16 April 2010, Volume 14 Issue 16 Previous Issue    Next Issue
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    Effect of personalized prefabricated titanium template, autologous bone and nano-hydroxyapatite on reconstruction of maxilla defect in rabbits
    Wang Zhi-ying, Li Shi-de, Ma Xiao-lin, Zhao Bing-jing, Jin Ding, Ge Yi-ming, Qi Feng
    2010, 14 (16):  2851-2854.  doi: 10.3969/j.issn.1673-8225.2010.16.001
    Abstract ( 119 )   PDF (1336KB) ( 457 )   Save

    BACKGROUND: Autologous bone, bone substitute materials and guided bone regeneration (GBR) technique can repair jaw defects, but the absorption speed of bone substitute materials and GBR membrane are faster than the formation speed of new bone, therefore, it affects the volume and shape of new bone.
    OBJECTIVE: To investigate the role of personalized prefabricated titanium template, autologous bone and nano-hydroxyapatite on restoration of maxillary defect in rabbit.
    METHODS: A total of 18 rabbits were randomly divided into two groups, and maxillary alveolar defect with 10 mm length and    5 mm high was created. The template was implanted in both two groups, and fastened with titanium screws. Autologous and nano-hydroxyapatite were placed into the defect in experimental group; neither autologous bone nor bone substitute materials were implanted into the defect in control group. New bone formation, X-ray findings, and histological changes with HE stain were carried out 4, 8 and 12 weeks postoperatively.
    RESULTS AND CONCLUSION: The quality of new bone in experimental group was batter than that in control group 4 weeks postoperatively, but the quality of new bone was almost the same 8 and 12 weeks postoperatively. By paired t-test, there was significant difference in new bone density between the experimental group and the control group 4 weeks after operation (P < 0.01), but there was no significant difference in new bone density between the experimental group and the control group 8 and 12 weeks after operation (P > 0.05). Autologous bone and nano-hydroxyapatite can restore the defect of maxillary alveola. Personalized prefabricated titanium template can play an important role of screen membrane and external scaffold in new bone formation, and remain shape of new bone.

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    Biomechanical evaluation of repairing rabbit cancellous bone defect by implanting porous carbonated hydroxyapatite cement
    Yao Qi, Hao Li-bo, Mao Ke-ya, Tang Pei-fu, Wang Ji-fang
    2010, 14 (16):  2857-2860.  doi: 10.3969/j.issn.1673-8225.2010.16.003 
    Abstract ( 139 )   PDF (1418KB) ( 429 )   Save

    BACKGROUND: A new material of porous carbonated hydroxyapatite cement (PCHC) is discovered using foaming technique. The new material characterizes original solidification and forms porous structure.
    OBJECTIVE: To investigate the biomechanical effect of PCHC on repairing cancellous bone defect.
    METHODS: Among 30 New Land rabbits, 25 ones were considered as surgery group, whose bilateral condyles of femur was used to establish bone defect model (5.5 mm diameter and 12 mm depth). PCHC was implanted into the left side, which was considered as the experimental group, and carbonated hydroxyapatite cement (CHC) was implanted into the right side, which was considered as the control group. Another 5 rabbits were used as normal mechanical control group. Both PCHC and CHC were dip in simulated body fluid (SBF) to test mechanical intension. PCHC and CHC were then implanted into muscles of back in the surgery group. Rabbits were sacrificed after 2, 4, 8, 12, and 16 weeks postoperatively. Mechanical analysis was tested following intra-bone and intramuscular implantation, and compressive strength was then tested following dipping into SBF.
    RESULTS AND CONCLUSION: PCHC: Intra-bone mechanical strength was lower at 2 weeks, the lowest at 4 weeks, but then closed to intension of normal cancellated bone at 8 weeks, higher than normal cancellated bone at 12 weeks, and recovered to the level of normal cancellated bone at 16 weeks. CHC: Intra-bone strength was higher than that of PCHC at 2 weeks, decreased at 4 weeks, gradually increased at 8, 12, and 16 weeks, but still lower than intension of normal cancellated bone. Compressive strength of both PCHC and CHC was not changed following dipping in SBF; however, compressive strength was changed remarkably following intramuscular implantation. The results demonstrated that PCHC characterized by immobilization in situ and mechanical supporting. Thus it could be used for one kind of bone substitute material to repair the bone defect.

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    Chondrogenesis of bone marrow mesenchymal stem cells, chondrocytes and adipose-derived adult stromal cells in three-dimensional scaffolds
    Chen Tie, Zhang Ling-min, Chu Bin, Tang Shun-qing
    2010, 14 (16):  2861-2863.  doi: 10.3969/j.issn.1673-8225.2010.16.004
    Abstract ( 67 )   PDF (951KB) ( 384 )   Save

    BACKGROUND: The key factor for cartilage repair is an orient differentiation of seed cells in three-dimensional scaffold; however, clear cartilage is hardly observed in the three-dimensional scaffold.
    OBJECTIVE: To investigate the chondrogenesis of bone marrow mesenchymal stem cells (BMSCs), chondrocytes (CCs) and adipose-derived adult stromal cells (ADAS) in three-dimensional scaffolds
    METHODS: BMSCs, CCs, and ADAS were primary-cultured from goat bone marrow following trypsin digestion and amplified in monolayer. P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.
    RESULTS AND CONCLUSION: The seeded cells had chondrogenesis in scaffolds under certain conditions. In details, BMSCs in 20% (W/W) hyaluronan/collagen I scaffolds could perform a hyaline-cartilaginous phenotype when they were expanded with less than 3 passages and cultured in a chondrogenic medium, and CCs did better than BMSCs in chondrogenesis with high passage number; however, ADAS just showed a minor chondrogenesis even in a pellet culture. BMSCs and CCs were better than ADAS as seeded cells for cartilage tissue engineering and regenerative medicine.

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    Repair of rabbit knee articular cartilage defect by the injectable chitosan/beta-glycerophosphate gel encapsulating allograft chondrocytes and the intervention of Weilingxian
    Ma Yong, Chen Jin-fei, Zhang Yun-shen, Wang Yan-ling, Jin Xiang, Xu Jian-an, Wang Jian-wei
    2010, 14 (16):  2864-2869.  doi: 10.3969/j.issn.1673-8225.2010.16.005
    Abstract ( 336 )   PDF (2007KB) ( 696 )   Save

    BACKGROUND: At present, studies on repair of cartilage defect have been focused on tissue engineering technique. Growth factors are one of the most important parts. However, the effect and security of growth factors have not been confirmed. Studies have shown that Weilingxian can maintain and promote the synthesis of proteoglycan, collagen Ⅱof chondrocyte, and it also can promote proliferation of chondrocyte and expression of transforming growth factor (TGF)-β1 mRNA.
    OBJECTIVE: To investigate the feasibility of injectable chitosan/β-glycerol phosphate (C/β-GP) encapsulating allograft chondrocytes on the repair of articular cartilage defects and the intervention effect and possible mechanisms of Weilingxian.
    METHODS: A 0.4-mm defect was established on knee articular cartilage. Expeirmental New Zealand rabbits were randomly divided into three groups: Weilingxian, common culture media, and model groups. In the common culture media group, the samples were treated with C/β-GP and chondrocyte suspension (1 mL); at 2 days after gel injection, Weilingxian or common culture media (1 mL) were respectively given into joint cavity, once a day, for 7 successive days. The samples in the model group were not treated. Gross, histological (HE staining, TB staining), typeⅡ collagen immunohistochemical, and Wakitani score examinations were performed on 6 and 12 weeks after surgery.
    RESULTS AND CONCLUSION: Defects of articular surface were well filled in Weilingxian and common culture media groups, and hyaline cartilage-like structure was formed. The surface flatness and degree of integration with surrounding tissue of Weilingxian group was better than common culture media group. Formation of cartilage-like and secretion of cartilage matrix and specificity of collagen typeⅡwere found in histological slices. Defects in the model group were not repaired, while tissue proliferative degeneration was observed. Integration of repair tissue with surrounding tissue, histology and amount of typeⅡcollagen secretion in Weilingxian group were better than common culture media group. Wakitani scores of Weilingxian group and common culture media group were significantly lower than model group (P < 0.01), and scores of Weilingxian group was significantly lower than common culture media group (P < 0.05). Injectable chitosan/β-glycerolphosphate gel encapsulating allograft chondrocytes could repair articular cartilage defects, and Weilingxian was able to promote the process of it, this manifested the role like growth factor in tissue engineering technique repairing articular cartilage defects.

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    Performance comparison among three biomaterials in repairing articular cartilage defects
    Guo Zhong-peng, Peng Chao, Jiang Dian-ming, An Hong
    2010, 14 (16):  2870-2874.  doi: 10.3969/j.issn.1673-8225.2010.16.006
    Abstract ( 103 )   PDF (1487KB) ( 396 )   Save

    BACKGROUND: Polyvinyl alcohol hydrogel (PVA) has been generally considered as a good substitute material of articular cartilage. Polyamide 66 (PA66) polymer has achieved an extensive application in the fields of engineering and medicine owing to its high intensity, strong tenacity and good stability. Polyurethanes exhibit good histocompatibility and blood compatibility and show distinct advantages in repairing cartilage. 
    OBJECTIVE: To compare the outcomes of repairing articular cartilage defects in rabbit knee joints with three materials and to find the best material.
    METHODS: Thirty-six healthy adult New Zealand rabbits were prepared for the models of articular cartilage defects by perforating on the patellofemoral joint and randomly divided into 4 groups, with 9 animals in each group. PVA, polyurethane, and PA66 were implanted into the into articular cartilage defects in rabbits of corresponding groups. There was no treatment in the control group. The macroscopic and histological evaluations of rabbit knee joints were performed at 4, 8 and 12 weeks postoperatively. The score of histology was used to evaluate the repairs of articular cartilage defects at 12 weeks postoperatively.
    RESULTS AND CONCLUSION: No infection was observed in all animals with good motion of joints. At 12 weeks postoperatively, the materials got well with surrounding cartilage, which did not degenerate obviously. The defects in the control group were filled with fibrous scar-like tissues that arranged in disorder. The histological score of the in the group implanted by PVA, as well as polyurethane, was higher than that in PA66 and the control groups (P < 0.05), but the difference between the two groups had no significance (P > 0.05). The porous materials PVA and polyurethane have good function for replacing articular cartilage, with good biocompatibility.

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    Construction of engineered myocardial tissues with polylactic-co-glycolic acid polymer and cardiomyocyte-like cells in vitro
    Xing Yu-jie, Lü An-lin,Wang Li, Yan Xue-bo
    2010, 14 (16):  2875-2878.  doi: 10.3969/j.issn.1673-8225.2010.16.007  
    Abstract ( 90 )   PDF (1303KB) ( 287 )   Save

    BACKGROUND: Previous research has demonstrated that bone marrow mesenchymal stem cells (BMSCs) differentiate into varying mesoderm-derived mesenchymal cells, such as myocardial cells.
    OBJECTIVE: To investigate the feasibility of construction of engineered myocardial tissues with polylactic-co-glycolic acid (PLGA) polymer and cardiomyocyte-like cells derived from BMSCs in vitro.
    METHODS: BMSCs were isolated from bone marrow of SD rats by density gradient centrifugation. The third passage cells were induced with the culture medium including 5-aza for 4 weeks. After successful induction, the cells were trypsinized and suspended, then the cell suspension was added to the polylactic-co-glycolic acid square slowly and cultured in the incubator for 14 days. The morphological changes were observed before and after induction under phase contrast microscope. The cardiomyocyte-like cells were identified by immunofluorescence staining. The naked eyes were used to observe the morphology of engineered myocardial tissues. The ultrastructures of the engineered myocardial tissues were viewed with a transmission electron microscope.
    RESULTS AND CONCLUSION: BMSCs of primary culture formed cell colonies at 14 days. The passaged cells were larger than those of primary culture. After induction by 5-aza, the cells presented long spindle and aligned in parallel. The expression of specific proteins of cardiac troponin I (cTnI) in induced BMSCs was positive. Transmission electron microscopy showed that the engineered myocardial tissues had myofilaments, Z line-like substances. With PLGA and cardiomyocyte-like cells derived from BMSCs, the engineered myocardial tissues which resemble native cardiac tissues can be successfully constructed in vitro.

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    Vascular endothelial growth factors in three-dimensional angiogenesis induced by rat tail collagen gel
    Shi Sen, He Yan-zheng, Song Li, Liu Yong, Yang Hui, Zhong Wu, Zeng Hong
    2010, 14 (16):  2879-2882.  doi: 10.3969/j.issn.1673-8225.2010.16.008
    Abstract ( 191 )   PDF (1280KB) ( 343 )   Save

    BACKGROUND: Angiogenesis attracts much attention in tissue engineering field. Previous research has proved that a two-dimensional culture of vascular endothelial growth factor (VEGF) promotes angiogenesis.
    OBJECTIVE: To evaluate the effect of VEGF on three-dimensional angiogenesis.
    METHODS: Endothelial progenitor cells were separated from the SD rat bone marrow. At about 70%-80% fusion, rat tail collagen gel was added to establish three-dimensional models. Samples in the experimental group were incubated in complete culture solution containing M199 culture media, fetal bovine serum, VEGF, and double antibody. The samples in the control group were incubated with VEGF-free culture media. In vitro culture and amplification of bone marrow-derived endothelial progenitor cells were determined at 1, 4, 7, and 20 days after incubation. Morphology and quantitative analysis were performed at 3, 6, 9, and 12 days after three-dimensional model establishment.
    RESULTS AND CONCLUSION: Endothelial progenitor cells grew from three-dimensional matrix into collagen matrix in the experimental group. Budding and infiltration were observed in the collagen within 24 hours, and branching-like structure was then gradually formed. Cells in the control group grew slowly, with slowing budding, small tubiform structure, superficial infiltration into collagen, sparse network structure, and non-intact. Numbers of newborn vessels in the experimental group were significantly greater than control group (P < 0.01). A detection on gel block showed positive expressions of endothelin-1 and endothelial nitric oxide synthase-3 on the 3rd, 6th, 9th, and 12th days. The results demonstrated that VEGF mobilized and induced endothelial progenitor cells in order to promote angiogenesis. Rat tail collagen gel induced endothelial progenitor cells which behaved migration, proliferation, and pullulation of angiogenesis.

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    Repairing articular cartilage and subchondral bone defects with polyurethane/ nano-hydroxyapatite+polyamide66 composite
    Peng Chao, Guo Zhong-peng, Jiang Dian-ming, An Hong
    2010, 14 (16):  2883-2887.  doi: 10.3969/j.issn.1673-8225.2010.16.009
    Abstract ( 88 )   PDF (1883KB) ( 443 )   Save

    BACKGROUND: Cartilage stent materials were abundant. With the development of preparation technique, structure, and surface modification technique, performance of materials were improved remarkably; thus, Integrated-repairing defects of articular cartilage and subchondral bone required high stability of cartilage replacement materials.
    OBJECTIVE: To evaluate the effect of polyurethane/ nano-hydroxyapatite+polyamide66 (PU/n-HA+PA66) biological composite material on repairing articular cartilage and subchondral bone.
    METHODS: Twenty dog models were randomly divided into 4 groups: porous PU/n-HA+PA66 implant group, compact PU/n-HA+PA66 implant group, n-HA+PA66 implant group and blank control group. The macroscopic and histological evaluations were performed at 4, 12, and 24 weeks postoperatively. The interfaces of biomaterial implants with articular cartilage were observed by scanning electron microscope.
    RESULTS AND CONCLUSION: After 12 and 24 weeks, bone tissue grew into lower layer materials. Porous PU was well fused with peripheral cartilage. At 24 weeks postoperatively, the porous PU was closely connected with surrounding articular cartilage than the compact PU, and the articular cartilage surrounding the compact PU degenerated obviously. PU/n-HA+PA66 had a similar structure with normal cartilage and subchondral bone and had remarkable effects on repairing defects of articular cartilage and subchondral bone.

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    Preparation and in vivo and in vitro biocompatibility of acellular vascular matrix
    Yu Gao-sheng, Dong Jian-de, Li Chun-min, Chen Xiao-bo
    2010, 14 (16):  2888-2890.  doi: 10.3969/j.issn.1673-8225.2010.16.010
    Abstract ( 136 )   PDF (964KB) ( 395 )   Save

    BACKGROUND: Acellular vascular matrix as vascular scaffold has following advantages: acellular vascular matrix possesses complicated three-dimensional structure of natural blood vessels. Growth factor and structural domain on the surface of acellular matrix helps for cell adhesion and infiltration.
    OBJECTIVE: To prepare acellular vascular matrix material and to evaluate its biocompatibility in vivo and in vitro.
    METHODS: Trypsin and Triton X-100 were used to gradually dispose pig carotid artery and to prepare acellular vascular matrix. The biocompatibility of the material was evaluated by implantation in muscle, acute toxicity experiment and cytotoxicity test in vitro.
    RESULTS AND CONCLUSION: The acellular vascular matrix material possessed good chemical stability and did not release harmful factors that produced destruction and dissolution in erythrocytes, without acute hemolytic reaction or toxic effects on cell growth. The acellular vascular matrix material showed lots of inflammatory cell infiltration in early stage of implantation, and no significant inflammatory cell infiltration in late stage of observation. Fibroblasts were visible in the acellular matrix. In addition, the acellular matrix material did not exhibit toxic effects on surrounding tissues, showing wound stage I healing. Simultaneously, histological sections demonstrated that there were good compatibility of scaffold material and surrounding tissues, without rejection. These indicated that acellular matrix material presented good biocompatibility in animals.

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    Gelatin treatment of calcium phosphate porous ceramics surface and in vitro cytocompatibility
    Liu Bin, Dong Yin-sheng, Lin Ping-hua, Zhao Hao-feng, Zhang Jun
    2010, 14 (16):  2891-2894.  doi: 10.3969/j.issn.1673-8225.2010.16.011
    Abstract ( 77 )   PDF (1633KB) ( 345 )   Save

    BACKGROUND: Sintered porous calcium phosphate ceramics is still insufficient in the induction of cell adhesion and growth due to its dense surface structure.
    OBJECTIVE: To fabricate calcium phosphate porous ceramics with high porosity, to treat their surface by gelatin, and to study the influence of surface treatment on cytocompatibility.
    METHODS: Calcium phosphate porous ceramics were fabricated by the foam impregnation technology firstly, using HA and β-TCP as raw materials. Then the porous ceramics were treated by 5wt% gelatin solution. The changes of pore property and surface morphology were characterized by scanning electron microscopy (SEM). The porosity of samples was measured by the Archimedes method. The compressive strength was measured with a WD-10A electronic universal material testing instrument. The rabbit bone narrow stromal cells were cultured on samples before and after surface treatment in vitro. MTT assay was performed for cells proliferation and SEM was used to observe the morphology of cells on porous ceramic.
    RESULTS AND CONCLUSION: After surface treatment, gelatin coatings formed uniformly on the pore walls of porous ceramic samples without changing their porous characteristics obviously. However, the average compressive strength value of samples was improved from (1.04±0.15) MPa to (5.17±0.17) MPa. In vitro, good cytocompatibility of samples before and after surface treatment was verified by the results of SEM and MTT. Moreover, the attachment, proliferation and activity of cells on the surface of samples treating with gelatin were better than those of sintered porous ceramics. Without destroying porous characteristic of samples, surface treatment with gelatin not only strengthened porous ceramics, but also improved cytocompatibility of the samples.

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    Biocompatibility for nano-zirconium dioxide-toughened hydroxyapatite
    Yu Xue-liang, Tang Yue-jun, Cao Mei-yu, Tang Yue-feng, Zhou Zhong-hua, Lü Chun-tang
    2010, 14 (16):  2895-2898.  doi: 10.3969/j.issn.1673-8225.2010.16.012
    Abstract ( 164 )   PDF (1486KB) ( 466 )   Save

    BACKGROUND: Previous research has investigated the effect of nano-zirconium dioxide-toughened hydroxyapatite (nano-ZrO2-HA) on the proliferation and differentiation of rabbit bone marrow stromal cells.
    OBJECTIVE: To evaluate the biocompatibility of nano-ZrO2-HA compound.
    METHODS: The experiments of acute toxicity, subacute toxicity, pyrogen, hemolysis, and intramuscular implantation were performed on New Zealand rabbits, healthy adult Kunming mice, and adult rats according to “Technical Evaluation Standards of Biomedical Materials and Medical Instruments”, promulgated by Chinese Board of Health.
    RESULTS AND CONCLUSION: Acute toxicity: All experimental animals survived. There was no significant difference in body mass before and after testing (P > 0.05). Pyrogen: Heating reaction was not tested. Hemolysis: Generally speaking, hemolytic crisis was not observed after 1 hour, and hemolytic rate was less than 5%. Intramuscular implantation: Infection did not occur in any animals, and materials were not discharged at all. Four weeks later, muscles were closely integrated with materials. A certain quantity of tissue grew into material pore, and peripheral muscle still had normal morphology and structure. Subacute toxicity: There was no significant difference in body mass and blood routine before and 2 weeks after testing. HE staining demonstrated that necrotic focus and other lesion were not observed in heart, liver, and kidney tissues under optic microscope. The results suggested that nano-ZrO2-HA was non-toxicity, and it had no pyrogen and hemolysis effect, as well as it did not stimulate to the muscle of rabbit. Inflammatory rejection did not happen to the animal. The nano-ZrO2-HA was closely integrated with the muscle, characterizing by great biocompatibility. Therefore, it can be used as substitution materials in clinical experiment. But it still needs to be evaluated completely.

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    Sensitization test of magnalium at the maximal dosage
    Chen Xu-qiong, Yin Qing-shui, Zhang Yu, Xia Hong, Zhang Tao, Yang Ke, Zhang Bing-chun, Tan Li-li, Lin Shan
    2010, 14 (16):  2899-2902.  doi: 10.3969/j.issn.1673-8225.2010.16.013
    Abstract ( 141 )   PDF (1108KB) ( 558 )   Save

    BACKGROUND: Magnalium which is potential to be the medical biodegraded metal implant is more and more interesting, but it must be well biocompatibility to human body.
    OBJECTIVE: To evaluate the sensitization of magnalium (AZ31B).
    METHODS: A total of 35 guinea pigs were randomly divided into saline group (negative control group, n = 10), 5% volume of formaldehyde (positive control group, n = 10), and AZ31B group (n = 15). Sensitization test at the maximal dosage was performed according to “Biological evaluation of medical devices-Part 10: Tests for irritation and delayed-type hypersensitivity”, including intracutaneous induction, local induction, and provocation. Patch was removed after 6, 24, 48, and 72 hours, and the skin response was classified according to Magnusson and Kligman criteria. Patch was removed after 72 hours, and skin was performed with biopsy, stained with HE staining, and observed under optic microscope.
    RESULTS AND CONCLUSION: Sensitization response was not tested in both negative control group and AZ31B group at 24, 48, and 72 hours after patch removal; however, moderate erythema was observed in the positive control group. Optic microscope demonstrated that criteria of allergy such as spongiosis, edema, and diffuse as well as perivascular mononuclear infiltration was not observed in the AZ31B group, but a few basophilic cells were observed. This suggested that AZ31B was biologically safe for sensitization.

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    Biocompatibility of electro-spinning beta-tricalcium phosphate/gelatin guided tissue regeneration membrane versus polylactic acid and poly(lactide-co-glycolide) membrane
    Zheng Jun-yuan, Tang Ming, Deng Xu-liang, Yang Xiao-ping, Zhang Shen, Li Xiao-xia
    2010, 14 (16):  2903-2906.  doi: 10.3969/j.issn.1673-8225.2010.16.014
    Abstract ( 147 )   PDF (1353KB) ( 364 )   Save

    BACKGROUND: In the past, guided tissue regeneration materials were produced by β-tricalcium phosphate (β-TCP) and gelatin (Gel), but the production of fiber membrane by electro-spinning technique was reported less.
    OBJECTIVE: To prepare a new kind of Gel with β-TCP hybrid nanofibrous membrane and testing its biocompatibility compared with polylactic acid (PLLA) and poly(lactide-co-glycolide) (PLGA) membrane.
    METHODS: The β-TCP/Gel guided tissue regeneration membrane was made by electro-spinning technique. Scanning electron microscopy (SEM) was employed to observe membrane surface. MTT test was performed to compare toxicity among β-TCP/Gel, PLLA, and PLGA membranes.
    RESULTS AND CONCLUSION: The β-TCP/Gel membrane was porous, and β-TCP granules were nodosity-adhered on surface of Gel. The average diameter of fiber was 500-600 nm. The distribution of fiber ranged from 200 to 500 nm. There was no significant difference in toxicity among PLLA, PLGA, and negative control group (P > 0.05). The results suggested that the β-TCP /Gel membrane was low cytotoxicity and suitable for tissue engineering. It would be a promising material for periodontal tissue regeneration.

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    Anticoagulation properties of sulfated agarose
    Jie You-ping, You Ling-ling, Liang Wan-ai, Tang Shun-qing
    2010, 14 (16):  2907-2910.  doi: 10.3969/j.issn.1673-8225.2010.16.015 
    Abstract ( 82 )   PDF (1132KB) ( 387 )   Save

    BACKGROUND: Polysaccharide sulfate has been hot focus in recent research. Regarding anticoagulant property of sulfated polysaccharides, recent studies mainly explored degree of substitution, and there are rare studies concerning relative molecular mass and spatial structure.
    OBJECTIVE: To study the anticoagulant activity of the sulfated agarose with different relative molecular masses.
    METHODS: With formamide as dispersing agent, agarose was sulfated with the method of chlorosulfonic acid-pyridine. The product was graded and purified with dialysis bag. Two kinds of sulfated agarose with different molecular masses were obtained, and their anticoagulant properties were checked by assays of the activated partial thromboplastin time, thrombin time.
    RESULTS AND CONCLUSION: Two kinds of sulfated agarose had similar degree of substitution and sulfation position, whereas their molecular weight was different. The bioassay results of coagulation index demonstrated that the anticoagulant activity of agarose improved with the increasing of molecular mass within a certain range. Relative molecular mass had an important effect on the anticoagulant activity of the sulfated agarose.

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    Effects of oral biofilm on guided bone regeneration in dental implant
    Xie Miao-miao, Zhao Bao-dong, Wang Wei-ying, Yu Long, Zhang Xiao-qi
    2010, 14 (16):  2911-2915.  doi: 10.3969/j.issn.1673-8225.2010.16.016
    Abstract ( 236 )   PDF (2037KB) ( 1334 )   Save

    BACKGROUND: Heal-all oral biofilm is a material utilized in repairing oral mucosa and soft tissues defects and characterized by degradation, easily preparation, long preserved duration, convenient transportation and good ossification, which has been widely used in dental implant as guided bone regeneration materials.
    OBJECTIVE: To check the clinical effective of Heal-all oral biofilm on guided bone regeneration in dental implant.
    METHODS: A total of 72 patients with bone defects in the implantation area were selected as subjects, who were divided into control group and experimental group at random. Bone defects around implants were repaired by guided bone regeneration technique with BME-10X medical collagen membrane and Heal-all oral biofilm respectively. X-ray and clinical examination were taken at 1 and 3 months after implantation. The amount of new-formed bone tissue was evaluated when stage II operation was performed.
    RESULTS AND CONCLUSION: In stage II operation, osseointegration was formed between implants and bone tissue in all 72 patients. The average rate of bone formation was 92% in the experimental group while 91% in the control group. All implants were successfully repaired with implant denture. Occlusal function was restored successfully with all 72 implants during the follow-up period of 3-24 months after restoration. As an alternative option of BME-10X medical collagen membrane, Heal-all oral biofilm can be used in guided bone formation clinically.

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    Cytotoxicity of a new type of antibiotic stainless micro-screw implant
    Zhang Dan, Zhang Yang, Lu Li, Yang Ke, Xue Nan
    2010, 14 (16):  2916-2920.  doi: 10.3969/j.issn.1673-8225.2010.16.017
    Abstract ( 111 )   PDF (1900KB) ( 391 )   Save

    BACKGROUND: Prevention of implant from inflammation was an effective method to reduce expulsion rate of stainless steel micro-screw implant, and develop new type of antibiotic material.
    OBJECTIVE: To evaluate the cytotoxicity of a new type of antibiotic stainless steels.
    METHODS: Metal test samples (antibiotic stainless steel, medical stainless steel, and medial pure titanium) were made into rectangular solids with length of 15 mm × 10 mm × 3 mm. Samples were cleaned with high temperature and high pressure. Alloy leaching liquor was prepared with DMEM culture media according to the ratio between surface area and volume of culture solution (3 cm2/mL). The leaching liquor was maintained in incubator at 37 ℃ for 96 hours, and then degerming was performed using microporous membrane. 6.4% phenol was added, which was considered as the positive control group, and DMEM culture media was considered as the blank control group. Growth of MG63 cells was observed under inverted phase contrast microscope; absorbance of cells cultured for 24, 48, 72, 96, and 120 hours was detected using MTT test; cytotoxicity of antibiotic stainless steels was evaluated.
    RESULTS AND CONCLUSION:  ① At 24 hours after culture, cells in the positive control group was abnormal; while, cells in other groups were well adherent-grew. ② After 48 hours of culture, with the culture time increased, cytotoxicity was detected out in the positive control group; cells in other groups and blank control groups were normal and grew well. A few of cells in stainless steels group showed karyopyknosis. ③ The absorbance was the highest of medical pure titanium, and then of antibiotic stainless steel and of medical stainless steel, while there was no significant difference between the three materials. ④ The level of cytotoxicity was grade 0. The results suggested that the antibiotic stainless steel which had the same cytotoxicity grade as medical stainless steel and pure titanium was in line with the requirement of its clinical application.

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    Effectiveness of filling ex vivo mandibular central incisor using bypassing root canal preparation with two kinds of warm gutter fillings and cold lateral condensation technique
    Qi Lu, Wu Pei-ling
    2010, 14 (16):  2921-2924.  doi: 10.3969/j.issn.1673-8225.2010.16.018
    Abstract ( 166 )   PDF (533KB) ( 362 )   Save

    BACKGROUND: The filling compactness can not be guaranteed due to apparatus retention in traditional lateral condensation. The warm gutter filling can achieve good results because of the easy changeable of gutta-percha in softening states.
    OBJECTIVE: To compare the warm gutta-percha fillings (Obtura Ⅱ and BeeFill 2 in1 filling) and cold lateral compacted gutta-percha with the bypassing root canal preparation in the intracanal separated instrument of oval canal.
    METHODS: Firstly, the 60 oval root canals of flat mandibular central incisors were randomly divided into three groups, with 20 teeth in each group. After that, the root canal was filled with BeeFill 2 in1, Obtura Ⅱ condensation, and lateral condensation. The percentage of void at 5-mm to root tip versus cross section area was observed under a scanning electron microscope.
    RESULTS AND CONCLUSION: Under in vitro condition, the difference of percentage of void area versus root canal area between the Obtura Ⅱ condensation group and the lateral condensation group had significance (P = 0.013), and the similar difference could be found between the BeeFill 2 in1 condensation group and the lateral condensation group (P = 0.014), but the difference had no significance between the BeeFill 2 in1 condensation group and the Obtura Ⅱ condensation group (P = 0.088). The results revealed that the root canal can be sealed accurately when establish hypass root next to the separated instrument and filled with BeeFill 2 in1 warm gutter filling.

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    Effect of three bonding adhesives on the orthodontic bracket loss rate in treating moderate and severe dental fluorosis
    Jia Han, Zhu Xin-yi
    2010, 14 (16):  2925-2928.  doi: 10.3969/j.issn.1673-8225.2010.16.019
    Abstract ( 139 )   PDF (452KB) ( 376 )   Save

    BACKGROUND: Because of the special structure of enamel fluorosis, the traditional bonding adhesives often lower the bonding strength and increase bracket loss rate, which affected the therapeutic effects seriously.
    OBJECTIVE: To observe the clinical bracket loss rate differences among the 3 different bonding adhesives in treating moderate and severe dental fluorosis.
    METHODS: A total of 15 cases (total 288 teeth) with dental fluorosis were selected, including 6 males and 9 females, aged 13-26 years. All cases were randomly divided into 3 groups, and treated by using Beijing-Tianjin enamel adhesive bonding brackets, photo-curing-flowable Resin + Single Bond 2 adhesive bonding brackets and a small amount of dental fluorosis of enamel surfaces were ground and bonding bracket, the loss rate of brackets were observed.
    RESULT AND CONCLUSION: The total loss rates of the Beijing-Tianjin-enamel adhesive bonding bracket group, photo-curing flowable resin + Single Bond 2 adhesive bonding bracket group, and a small amount of dental fluorosis of enamel surfaces were ground and bonding bracket group were 65.6%, 20.5%, and 2.5%, respectively. There was significant difference in bracket loss rate among bonding adhesives (P < 0.01). It suggested that a small amount of surface enamel were ground and bonding bracket got the best effect, followed by photo-curing flowable resin + Single Bond 2 adhesive bracket bonding group, both of them were significantly better than the Beijing-Tianjin-enamel adhesive bracket bonding group. Because veneering directly requires to grind part of the tooth structure which is difficult to accept for some patients, therefore, under the premise of not destroying the tooth structure, using photo-curing flowable resin + Single Bond 2 adhesive bonding of brackets instead of the traditional Beijing-Tianjin enamel adhesive bonding of brackets can effectively reduce the loss rate of brackets for moderate and severe dental fluorosis.

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    Evaluation of heat-activated and common nickel-titanium wire for orthodontic treatment
    Ji Li, He Hong, Zhong Xiao-long, Huang Dai-ying
    2010, 14 (16):  2929-2932.  doi: 10.3969/j.issn.1673-8225.2010.16.020
    Abstract ( 858 )   PDF (620KB) ( 613 )   Save

    BACKGROUND: Heat-activated nickel-titanium (HANT) wire is a new type of arch wire in orthodontic treatment, characterizing by well memory alloy ability and uneasily deformation. However, environmental temperature may affect its performance. 
    OBJECTIVE: To compare the effects of HANT and common nickel-titanium wire in clinical application.
    METHODS: A total of 80 patients who have accepted and finished orthodontic treatment in the First Affiliated Hospital of Sun Yat-sen University from July 2006 to November 2008 were randomly divided into two groups. The common group was treated with common arch wire in aligning and leveling stages, while the HANT group was treated using HANT in aligning and leveling stages. Tooth extracted cases were matched to non-tooth extracted cases. Indicators, such as time span for alignment, total treating time, times for wire breaking, times for bracket shedding, and times for return visits, were measured.
    RESULTS AND CONCLUSION: There was significant difference in treating time and times for wire breaking between the two groups (P < 0.05), but there was no significant difference in the times for bracket shedding. Anaphylactic response, toxic reaction, and other adverse reactions were not observed in the HANT group. Therefore, HANT wire can simplify orthodontic operation, shorten operation time beside the chair, save the total treating time, and reduce chances of shedding brackets and breaking wires.

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    Effect of magnesium content on microstructure and properties of powder metallurgy Ti-Mg alloy
    Zhang Jin-long, Tao Jie, Wang Yue-qin, Guo Xun-zhong, Wang Tao
    2010, 14 (16):  2933-2936.  doi: 10.3969/j.issn.1673-8225.2010.16.021
    Abstract ( 103 )   PDF (670KB) ( 431 )   Save

    BACKGROUND: High-strength titanium alloy can be prepared by using powder metallurgy technique, but there are still some problems such as containing toxic elements or high elastic modulus in prepared alloys.
    OBJECTIVE: A new Ti-Mg alloy with non-toxic and elastic modulus which is close to human bones and strengths satisfy the requirements for human body implants was successfully prepared by means of powder metallurgy technology, and the effects of magnesium content on the microstructure and mechanical properties of Ti-Mg alloy were systematically studied.
    METHODS: The Mg powder and Ti powder in different mass ratios were prepared by powder metallurgy process to get a new integrated medical Ti-Mg alloy with excellent comprehensive properties. The porosity, fracture surface morphology and phase constituents of samples were observed by optical microscopy, scanning electron microscopy and X-ray diffraction, and the mechanical properties including bending strength, impact toughness and hardness of the alloys were measured.
    RESULTS AND CONCLUSION: Magnesium mass increasing firstly decreased and then increased admissible strain. When magnesium mass was 10%, admissible strain was 0.97%, which was highly closed to 0.67% of cortical bone. The porosity decreased from 18.3% to 3.8%, the holes became more tact and distributes more uniform, with the increased of mass ratio of magnesium. And with the increased of mass ratio of magnesium, the hardness, bending strength and elastic modulus of alloys with similar pattern of change, there were little changed when the mass fraction of magnesium less than 10%, they decreased significantly and then tended to stable with the continued to increased of magnesium mass fraction. While the admissible strain firstly decreased and then increased, with the increased of the mass fraction of magnesium. When the mass fraction of magnesium was 10%, the hardness, bending strength, impact toughness satisfied the mechanical requirements for human body implants, and the bending strength and admissible strain were close to human bones, which indicated well biomechanical compatibility.

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    Investigation of pain during risperidone microsphere injection and at injection sites: A 12-week multi-center evaluation
    Wang Xue-jing, Li Ling-zhi, Xiao Wei-dong, Du Bo, Mi Wei-feng
    2010, 14 (16):  2937-2940.  doi: 10.3969/j.issn.1673-8225.2010.16.022 
    Abstract ( 160 )   PDF (585KB) ( 367 )   Save

    BACKGROUND: As the first long-acting atypical antipsychotics, the therapeutic effect and safety of risperidone microsphere have been proved. However, it may lead to serious pain due to the deep intramuscular injection.   
    OBJECTIVE: To evaluate the pain levels by 12-week injection of risperidone microsphere and to explore the relationship among dose and times of injection of risperidone microsphere and pain levels.
    METHODS: A total of 57 patients diagnosed as schizoprenia by DSM-Ⅳ, aged 18-65 years, were selected and injected risperidone microsphere once every 2 weeks with doses of 25, 37.5 and 50 mg. The pain levels were evaluated using 100 mm visual analogue scale during injection and at the injected sites. The effects of injected dose, injected frequency and injected sites on the pain were analyzed by the nurse questionnaire. 
    RESULTS AND CONCLUSION: The pain levels among the different doses groups had no notable differences (F = 1.35,       P > 0.05), which demonstrated that the pain had no relationship with injected dose. However, the pain level of injected sites had correlation to injected doses. The pain level of the 50 mg group was greater than that of the 37.5 and 25 mg groups. Accordingly, patients who treated by high dose of risperidone microsphere should be intervened by nurses.

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    Reconstruction of anterior cruciate ligament and graft selection
    Hao Jian-xue, Gao Shi-jun, Liu Suo-li, Wan Jian-she
    2010, 14 (16):  2941-2944.  doi: 10.3969/j.issn.1673-8225.2010.16.023
    Abstract ( 110 )   PDF (561KB) ( 398 )   Save

    BACKGROUND: Graft selection and histological fate for anterior cruciate ligament reconstruction is a hot topic in the fields of reconstruction and repair of anterior cruciate ligament.
    OBJECTIVE: To review structure of anterior cruciate ligament and graft selection.
    METHODS: Articles were retrieved from Medline database with the key words of “Anterior cruciate ligament, implant, reconstruction” between January 1980 and January 2010. Inclusion criteria: ① Reconstructive surgery of anterior cruciate ligament injury; ② graft selection of anterior cruciate ligament. Exclusion criteria: ① the old literatures; ② repetitive studies. A total of articles related to reconstruction of anterior cruciate ligament were retrieved, but 33 ones were included in the final analysis. The old, duplicated, and similar studies were excluded.
    RESULTS AND CONCLUSION: At present, the major therapy for anterior cruciate ligament injury includes arthroscopy and arthroscopy-assisted reconstruction. For clinical application, there are a lot of grafts, including autogenous grafts, allografts, heterologous allograft, biological materials, artificial materials and tissue engineering grafts. Autogenous semitendinosus tendon and gracilis tendon or autogenous bone - patellar tendon (middle 1/3) - bone (BPTB) are mainly used for anterior cruciate ligament reconstruction at home and abroad.

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    Research and progress of microcarrier culture technology
    Zhou Yan, Liu Bao-lin, Yang Bo, Wang Xin, Peng Cheng-hong,Han Bao-san
    2010, 14 (16):  2945-2948.  doi: 10.3969/j.issn.1673-8225.2010.16.024
    Abstract ( 69 )   PDF (695KB) ( 593 )   Save

    BACKGROUND: Microcarrier culture technology has become a new and large scale cell culture technology. It has been mainly used in the amplification research of tissue engineering seed cells. Microcarrier possesses the advantage of larger surface area and plays an essential role in microcarrier culture technology.
    OBJECTIVE: To sum up the biomaterials and methods of microcarrier preparation, and provide theoretical foundation for the study of microcarrier culture technology and tissue engineering.
    METHODS: Articles were retrieved from PubMed, Wanfang, and VIP databases with the key words of “microcarrier, biomaterials cell culture, tissue engineering” in both English and Chinese between 1967/2009 and 1990/2009, respectively. Inclusion criteria: study addressing microcarrier materials, preparation, and performance; study of microcarrier cell culture; animal experiments and clinical applications. A total of 34 articles were originally retrieved based on their titles and abstracts.
    RESULTS AND CONCLUSION: Although a lot of studies have reported research and preparation of microcarrier, clinical application remains still difficult. Recently, varying materials will be made into novel compound materials by new technology, which can adjust mechanics and biodegradation of microcarrier via surface modification.

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    Clinical characteristics of pelvic reconstruction using biomaterial patch in females
    Zhao Yong-hong, Hu Jin-ping
    2010, 14 (16):  2949-2952.  doi: 10.3969/j.issn.1673-8225.2010.16.025
    Abstract ( 56 )   PDF (719KB) ( 453 )   Save

    BACKGROUND: With wide application of biotechnological substitute materials, pelvic repair and reconstruction develop to a certain degree. Biomaterial patch is a major substitute for repairing injured pelvic fascia tissue, so it is widely used for pelvic reconstruction.
    OBJECTIVE: To investigate the feasibility, efficacy, and clinical value of biomaterial patch to pelvic reconstruction in the females.
    METHODS: Articles related to pelvic functional disorder, pelvic reconstruction, and application of patch implant were retrieved from PubMed (http://www.ncbi.nlm.nih.gov/PubMed) and (http://www.wanfangdata.com.cn) with the key words of “reconstruction of whole pelvic floor, mesh, synthetic mesh implants” in both Chinese and English between 1990 and 2008. Duplication studies were excluded. A total of 54 articles were initially retrieved, and 17 ones were included in the final analysis.
    RESULTS AND CONCLUSION: Pelvic organ prohpse, which was a major symptom of pelvic disorder in the females, caused by defect of pelvic supporting structure, injury, and functional disorder. Traditional operation could not solve fundamental question. At present, substitute materials for pelvic repair and reconstruction mainly include biomaterial patch (self-substitute materials, homogeneity substitute materials, and heterogeneity substitute materials) and artificial patch. All of them could substitute the injured pelvic fascia tissue; therefore, they were major substitute materials of pelvic tissue and widely used for pelvic reconstruction. Patch which was used for pelvic reconstruction realized the recovery of anatomic structure and caused functional recovery, with simple and easy processing. Additionally, patch application did not prolong operative time and cause complication, but induced well tolerance, security and reliability, and remarkable short-term effect on patients. However, the long-term efficacy should be further studied. The modified pelvic reconstruction is clinically valuable for patients with varying prolapsed sites.

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    Effect of interaction of chemo-mechanics on the microstructure and properties of thermal-sprayed bioactive coatings
    Liang Jia-chun, Wang Wei-ze, Xuan Fu-zhen
    2010, 14 (16):  2953-2957.  doi: 10.3969/j.issn.1673-8225.2010.16.026
    Abstract ( 179 )   PDF (688KB) ( 246 )   Save

    BACKGROUND: With the bio-coated prosthesis implanted in the body by a complex interaction of chemo-mechanics, the cases of clinical revision increased.
    OBJECTIVE: To summarize the research progress of effect of interaction of chemo-mechanics on the microstructure and properties of thermal-sprayed bioactive coatings.
    METHODS: The relevant articles were retrieved from Elsevier with the key words of “apatite, coating, spraying” in English between January 1999 to November 2009. Meanwhile, the relevant articles were searched from database of Vip Information with the key words of “apatite, coating, spraying” in Chinese between January 1989 to November 2009. The articles that were highly related to the effect of chemo-mechanics on the microstructure and properties of thermal-sprayed bio-coatings were collected. The repetitive researching results and those with weak correlation were excluded.
    RESULTS AND CONCLUSION: It was shown that loading affected the dissolution of bio-coatings significantly. Tensile stress promoted the dissolution, while compressive stress inversely. In addition, the mechanical properties decreased after immersion in simulation body fluid. Moreover, the mechanical properties increased when it was implanted in bone tissue. However, there were only investigation of microstructure and properties of bio-coatings under simple loading. The coupling effect of complex loading, such as tension, torsion and fatigue, etc., and the specific chemical environment on the bio-coatings should be studied in order to ensure the integrity of its structure and properties. 

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    Construction of tissue-engineered blood vessel stent using different materials: Characteristics and effectiveness
    Yin Ming-di, Zhao Zheng-kai, Zhang Jian
    2010, 14 (16):  2958-2962.  doi: 10.3969/j.issn.1673-8225.2010.16.027
    Abstract ( 72 )   PDF (750KB) ( 484 )   Save

    OBJECTIVE: To evaluate the characteristics and application prospect of tissue-engineered blood vessel stent materials.
    METHODS: Using “tissue engineering, tissue engineering blood vessel, intravascular stent” both in Chinese and English as key words to search documents published between January 1993 and October 2009. Literatures concerning biomaterials and tissue engineered blood vessel were included. Repetitive research or Meta analysis was excluded. After selection, 30 documents were included to discuss the kinds and characteristics of tissue-engineered blood vessel materials.
    RESULTS: Cellular tissue matrix is a kind of ideal material for intravascular engineering, which had widely applied in tissue-engineered stent. The fibrin-based stents are characterized by good biocompatibility, biodegradation, compatibility, as well as promote vascularization and tissue repair. Gelatin can implement its own “vascularization” due to less antigenicity and can be fully biodegraded. Accordingly, both the natural biomaterials and synthetic polymeric material have certain limitations, thus, the combination of these two materials would construct a tissue-engineered blood vessel stent that has good characteristics. Nano-modified technology is hopeful to be applied in the future transplantation of tissue-engineered blood vessel stent.
    CONCLUSION: The tissue-engineered blood vessel developed rapidly in these years. However, as yet, there is not an ideal intravascular stent material. The natural biomaterials arose more attention, but the mechanical function of which is far from the stent requirement. Thus, to reach the aims of repair and reconstruction, new materials are the urgent need in tissue-engineered intravascular stent.

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    Repairing femur defect using biomaterials and its features evaluation
    Yan Feng, Yang Wei-liang, Yang Wei
    2010, 14 (16):  2963-2966.  doi: 10.3969/j.issn.1673-8225.2010.16.028
    Abstract ( 71 )   PDF (539KB) ( 374 )   Save

    OBJECTIVE: To evaluate the features and application of tissue engineered biomaterials in repairing femur defects, and to search an optimal femur substitution.
    METHODS: Papers published between January 1993 and October 2009 were searched using computer with key words of “tissue engineering, femur defect, mesenchymal stem cells, cytokines and materials” both in English and Chinese. Documents addressing biomaterials and tissue engineered bone in repairing femur defects were included. Repetitive research or Meta analysis was excluded. After that, 21 literatures were selected to discuss the features and application of tissue engineered biomaterials in repairing femur defects.
    RESULTS: Stem cells technology is obtaining seed cells from myeloid tissues, amplifying in vitro, combining with scaffold material, and in vitro constructing tissue engineered bones. The cultured cells could maintain osteoblast biological characteristics and fully fused with bone tissues at recipient sites, simultaneously, avoid immunological rejection. The combination of materials or modify the material surface could promote cells adhere to materials and increase the cellular biological activity. When transfecting seed cells to bone defect areas using certain carriers, the transplanted cells could form new bones and secret bone growth factor, induce determined osteogenic precursor cells differentiated into inducible ostegenic precursor cells, thereby, fasten the bone healing. The composite materials prepared by three-dimensional virtual surgical simulation and computer numerical control exhibited advantages of composite materials and histological anatomy conjunction, which had perfect appearance.    
    CONCLUSION: Currently, there is no material can fully meet the requirement of bone tissue engineering. Thus, promoting adhesion between cells and materials, increasing cellular biological activity, and maintaining biological functions by combing materials or modifying material surface are the research focuses.

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    Selection and evaluation of materials for skull repairing
    Jiang Fu-gang, Xiong Qing-rong, Wang Tian-yi, Li Xiang-cheng, Cen Ming, Xia Xue-wei
    2010, 14 (16):  2967-2970.  doi: 10.3969/j.issn.1673-8225.2010.16.029
    Abstract ( 124 )   PDF (686KB) ( 733 )   Save

    OBJECTIVE: To summarize the classification, property of skull repair material and the processing of related complications in the application of repairing and remodeling the skull.
    METHODS: The PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) and CNKI Database (www.cnki.net/index.htm) was searched by the first author using key words of “codubix, synmesh, and bone cement” both in English and Chinese. Articles whose content is related to the types, property, biocompatibility and its application effect of skull repairing materials were selected. In the same field, the articles published by authoritative journals or different races were preferred. The repetitive or obsolete literatures were excluded. After that 26 documents were included in this paper. 
    RESULTS: The bone cement exhibited good histocompatibility, however, it is difficult to be absorbed, thus, it only be used for repairing part of skull defects. The study found that titanium had good biocompatibility and could combine with the skull. Its application had a promising prospect, but there are many inadequacies. With the continuous deepening of bio-engineering, bone tissue engineering, and cartilage tissue engineering, it will provide a broader perspective for the study of skull repair materials.
    CONCLUSION: There are many kinds of materials for skull repairing, and this paper only introduces some of the widely used ones. Actually, the selection of repairing materials should consider the pathogenetic condition, economic condition, local equipment and technical levels. If possible, titanium mesh and titanium screw are preferred, which is characterized by easy operation, few complication and beautiful appearance. When using autogenous bone or bone cements, if related complications can be reduced and handled effectively, the results will be satisfactory. 

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    Reconstruction of LARS artificial ligament and anterior and posterior cruciate ligament sports injury of keen joint
    Wang Zhi-li, Cheng Chao, Hu Xiao-yan
    2010, 14 (16):  2971-2974.  doi: 10.3969/j.issn.1673-8225.2010.16.030
    Abstract ( 227 )   PDF (700KB) ( 763 )   Save

    OBJECTIVE: To investigate the effect of LARS artificial ligament on reconstruction of anterior and posterior cruciate ligament of keen joint.
    METHODS: Articles addressing reconstruction of anterior and posterior cruciate ligament by LARS artificial ligament were retrieved from VIP database (http://www.cqvip.com/) with the key words of “LARS artificial ligament, cruciate ligament, reconstruction” in Chinese between January 2000 and February 2010. Inclusion criteria: advantages and disadvantages of reconstructing anterior and posterior cruciate ligament by LARS artificial ligament; clinical application of reconstructing anterior and posterior cruciate ligament by LARS artificial ligament. Exclusion criteria: Duplicated studies or old references were excluded. A total of 31 articles were included in the final analysis.
    RESULTS: Reconstruction of anterior and posterior cruciate ligament by LARS artificial ligament characterized by convenient operation, short operative time, mild trauma, engaging in movement early, great strength, simple implantation, and reliable fixation; however, most patients were not able to bear the high medical cost, thus clinical application was limited. Scholars thought that LARS artificial ligament had great effects on reconstruction of anterior and posterior cruciate ligament.
    CONCLUSION: Patients who had multi-ligament injury, overhauling of ligament, and early movement were treated with LARS artificial ligament.

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    Tissue-engineered tendon repairing tendon tissue defect induced by movement training: A correlated research of cell materials
    Si Hong-yu
    2010, 14 (16):  2975-2978.  doi: 10.3969/j.issn.1673-8225.2010.16.031
    Abstract ( 157 )   PDF (635KB) ( 283 )   Save

    BACKGROUND: Traditionally, tendon was repaired by autoallergic tendon transplantation and transfusion; however, both methods were limited by supply region and additional trauma, and the development was slowed down.
    OBJECTIVE: To summarize biomechanical characteristics of seed cells, stent, and bioreactor during repairing tendon tissue defect, and provide prevention and cure of tendon injury induced by training and competition.
    METHODS: A computer-based online search was conducted in VIP database with the keywords of “movement training, tendon tissue, repair” from January 2004 to October 2009. Articles which were related to tendon tissue injury induced by movement training were included, but articles related to duplicated research or Meta analysis were excluded.
    RESULTS AND CONCLUSION: Tissue-engineered tendon has achieved therapeutic effects to a certain degree; however, it is still not used for clinical application. Source of seed cells was restrict to autoallergic tendon cells; on the other hand, comparison between fibroblast and tendon cells, gene chip was used to modify gene in order to solve source of seed cells of tissue-engineered tendon. During regeneration of tendon, normal mechanical stimulation played an important role in moulding of newborn tendon. Because the stimulation might induce orientation arrangement of newborn collagen fiber, enhance mechanical strength of newborn tendon, make sure the functional training as early as possibly, effectively reduce tendon adhesion, and provide an ideal stent for great circulation of tendon repairing. Scientific training is necessary for coaches and athletes to avoid from tendon tissue injury during movement training and competition. Although tendon tissue engineering has developed well, it still brings adverse effect on body and mind.

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    Performance of fiber post and its stickiness and retention during repairing residual root and crown
    Ren Zhong-bao, Guo Ya-feng, Tan Zhao-jun, Zhao Yue, Zhao Ning
    2010, 14 (16):  2979-2982.  doi: 10.3969/j.issn.1673-8225.2010.16.032
    Abstract ( 178 )   PDF (696KB) ( 661 )   Save

    OBJECTIVE: To investigate advantages and disadvantages of preexisting metal post, casting metal post, ceramic post, and fiber post for clinical application, and to evaluate mechanical function, esthetical performance, and biocompatibility of fiber post.
    METHODS: Articles were retrieved with the key words of “fiber post, post-core restoration, dental materials, cement material, binder” in both English and Chinese between January 1990 and October 2008. Inclusion criteria: Articles addressing clinical application of fiber post for repairing residual root and crown were included. Exclusion criteria: Duplicated studies or Meta analysis was excluded. A total of 24 articles were included to investigate mechanical function, biocompatibility, and stickiness and retention of fiber post.
    RESULTS: Traditionally, metal post was easily folded and corrosive; in addition, esthetical performance and biocompatibility were still dissolved. Intension, hardness, esthetical performance, corrosion proof, fatigue resistance, and biocompatibility of fiber post were superior to those of traditional metal post. Furthermore, fiber post was able to highly adhere to root canal dentin via resin splicing agent, which could prolong useful life of dental prosthetic restoration, reduce root folded onset, and benefit for dental storage and repairing. Light mass of fiber post was another advantage compared with metal post. If the repair was failure, fiber post was easily taken out and reused. The combination of fiber post and resin nucleus was beneficial for dental prosthetic restoration which was highly closed to original structure of natural teeth.
    CONCLUSION: Fiber post was characterized by suitable elastic modulus, great esthetical repairing effect, reliable stickiness, easy identification and removal, high achievement ratio of repairing, and convenient operation; therefore, fiber post was a new and ideal material for clinical application.

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    Physiochemical characteristics of resin restorative material and its clinical application in repairing dental caries
    Wang Wei-hua
    2010, 14 (16):  2983-2986.  doi: 10.3969/j.issn.1673-8225.2010.16.033
    Abstract ( 93 )   PDF (635KB) ( 420 )   Save

    OBJECTIVE: To evaluate the physiochemical characteristics of resin restorative material and its clinical application in repairing dental caries.
    METHODS: Related papers published between January 1995 and January 2010 were searched using key words of “dental caries, resin, restoration, dental pulp, wear resistance and hardness” both in English and Chinese from databases of Medline and Chinese Journal Full-text Database.
    RESULTS: As a kind of cosmetology repair material, the color of resin restorative material is similar to the natural teeth, and exhibits strong bonding force to enamel. But, the mechanical features of which is smaller than dental amalgam. The composite resin commonly comprised crystalline quartz and fumed silica, which had advantages of insufficient mechanical strength, poor wear resistance and great polymerization shrinkage. Recently, the research of resin gained breakthrough and newly typed composite resin was developed. Though the mechanical strength has been notably improved, it is still far from the requirement. The polymer-metal nano-resins, such as tantalum oxide nanoparticles and zirconia-based resin, can not only enhance the strength, decrease polymerization shrinkage, but also exhibit good appearance and surface properties, therefore are the  most promising materials. 
    CONCLUSION: At present, plenty of resin restorative materials were used in caries fillings with own advantages and disadvantages. Novel composite resin would be widely used in future due to its unique superiority.

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    Material features and biocompartibility of stent implantation in treating intrahepatic biliary stricture under ultrasonic guidance
    He Xiu-li
    2010, 14 (16):  2987-2990.  doi: 10.3969/j.issn.1673-8225.2010.16.034
    Abstract ( 126 )   PDF (660KB) ( 355 )   Save

    OBJECTIVE: To explore the material features and biocompartibility of stent implantation in treating intrahepatic biliary stricture under ultrasonic guidance.
    METHODS: The Chinese Journal Full-text Database and Medline were retrieved by the first author using search terms of “intrahepatic biliary stricture, stent, ultrasonic guidance, and biocompartibility” both in Chinese and English. Meta analysis, unrelated papers or repetitive studies were excluded. After that, 21 documents concerning the material features and biocompartibility of stent implantation in treating intrahepatic biliary stricture under ultrasonic guidance were analyzed.  
    RESULTS: Compared with the previousl surgery, stent implantation exhibited advantages such as small trauma, better curative effect, able to improve life quality and prolong life. Ultrasonic guidance has shown its clinical values during stent implantation because of accurate location, few traumas, low complication and cheap cost. The metal stents were preferred in treating lower ureteral obstruction, which is superior to plastic stents in preventing bacteria, maintaining stent patency and reducing complications. However, due to easy change and cheap cost, the plastic stents are widely used in treating intrahepatic biliary stricture, especially for patients within 3-month survival times.
    CONCLUSION: Materials of the stents play a key role in influencing the short- and long-term effectiveness in treating intrahepatic biliary stricture. Accordingly, the suitable stents should be selected prior to implantation according to the sites, degree, type of obstruction, physical state, expected survival time, economical ability, as well as the prognosis to reach the goals of relieving pain and prolonging life. 

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    Effects of materials and types of biliary tract stents on implantation effectiveness: Enhancing patency rate and prolonging patency time
    Zhao Xiao-jun
    2010, 14 (16):  2991-2994.  doi: 10.3969/j.issn.1673-8225.2010.16.035
    Abstract ( 154 )   PDF (607KB) ( 523 )   Save

    BACKGROUND: Biliary tract stents comprise two types: expanding metallic stents and plastic stents, the patency time of the former is shorter than the latter, but the cost is higher. Thus, it is dispute which kinds of stent should be selected. Accordingly, it becomes a research topic how to improve patency rate and prolong patency time using cheap stents.
    OBJECTIVE: To explain the progress of biliary tract stents and to explore the effects of materials and types of biliary tract stents on patency rates and patency times.
    METHODS: The Chinese Journal Full-text Database and Medline were retrieved by the first author using search terms of “biliary stent, metallic stent, plastic stent, occlusion and stricture” both in Chinese and English. Meta analysis or repetitive studies were excluded. After that, 20 documents concerning the effects of materials and types of biliary tract stents on patency rates and patency times were analyzed.  
    RESULTS AND CONCLUSION: Biliary stent implantation is the preferred way for alleviative treatment of malignant obstruction of bile duct with few complications. Biliary tract stents comprise two types comprise metallic stents and plastic stents, the biocompatibility of both stents need to be improved. The metallic stents exhibited advantages in preventing bacteria, maintaining stent patency and reducing complications, but the high cost limited its application. Though plastic stents easily lead to stent occlusion due to bacteria adhesion and biliary siltation, however, it has merits of easy change and cheap cost. The combination of biliary stent implantation and radiotherapy can prolong effective patency period and prevent stent occlusion. The results demonstrated that biliary stent implantation is best methods for treating malignant obstruction of bile duct, and combined biliary stent implantation with radiotherapy can prevent stent obstruction.

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    Application of polypropylene repair material in treating inguinal hernia
    Mi Ying-hua, Liu Ying, Wang Xiang
    2010, 14 (16):  2995-2998.  doi: 10.3969/j.issn.1673-8225.2010.16.036
    Abstract ( 111 )   PDF (740KB) ( 385 )   Save

    OBJECTIVE: To summarize the application of polypropylene repair material in treating inguinal hernia.
    METHODS: Papers regarding polypropylene repair material in treating inguinal hernia published in VIP data between January 1998 and October 2009 were researched using key words of “polypropylene, inguinal hernia, and complication”. Inclusion criteria: Repairing inguinal hernia using polypropylene materials. Experimental results concerning related complications. Exclusion criteria: repetitive study and relatively old literature. A total of 17 documents were included in this study.
    RESULTS: Organic polymeric materials were clinical utilized in repairing inguinal hernia at the end of 1950s. The polypropylene patch was knitted by single fiber or multi-strands fibers of polypropylene, and exhibited single layer network structures, which is preferred material for repairing abdominal-wall defect. Compared with other unabsorble materials, the polypropylene patch had following advantage: obviously stimulating fibroplasias; easy permeated by fibrous tissues and embedded into tissues; maintaining highly tensile strength following implantation; and relative low cost. Few material-related complications occurred following repairing, mainly presented with infection, serofluid swelling, ankylenteron, intestinal leakage, material displacement, as well as material shrinkage. 
    CONCLUSION: Polypropylene material is an ideal repair material due to good histocompatibility, few rejections, and anti-infection capability. It leads to easy operation, small trauma, fast recovery and few complications in repairing defects.

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    Material features, biocompatibility of coronary artery stent and related complications following implantation
    Hu Rong, Wang Ya-li
    2010, 14 (16):  2999-3002.  doi: 10.3969/j.issn.1673-8225.2010.16.037
    Abstract ( 69 )   PDF (777KB) ( 447 )   Save

    OBJECTIVE: To explore the material features and biocompatibility of coronary artery stent and related complications following implantation.
    METHODS: The databases of CNKI and Medline were searched by the first author using key words of “coronary artery stent, material, biocompatibility and complications” both in English and Chinese. Meta analysis or repetitive studies were excluded, and 16 papers were included in this analysis.
    RESULTS: Stent materials play an important role in reducing inflammatory reaction and restenosis following stent implantation. As a medical material for humans, the features of stent should be meet the requirements of reliable distensibility, mechanical persistence, good flexibility and geometrical stability, possible small diameter, excellent blood compatibility, as well as good corrosion resistance. All of these depended on the mechanical and physical features of materials.
    CONCLUSION: The study of drug-coating stent and drug-eluting stent reduced the restenosis rates following implantation, and the biodegradable stent also attracted more attention. When treating coronary heart disease with cardiovascular stent, the complications should be avoided except consideration of the successful rate of reperfusion.

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    Effect of vacuum sealing drainage and adsorbing material on treatment of large area of skin avulsion wound
    Li Yue-hui
    2010, 14 (16):  3003-3006.  doi: 10.3969/j.issn.1673-8225.2010.16.038
    Abstract ( 90 )   PDF (761KB) ( 434 )   Save

    BACKGROUND: Skin flap replantation was poor in patients with large area of skin avulsion due to poor blood transportation and severe infection; therefore, dressing should be changed again and again.
    OBJECTIVE: To investigate the effect of vacuum sealing drainage technology and absorbing materials on the large area of skin avulsion.
    METHODS: A total of 100 patients with large area of skin avulsion were selected from Department of Medical Beauty of Affiliated Hospital of Xingtai Medical College between September 1, 2008 and August 31, 2009. The injury area ranged from 10% to 50%. All patients were randomly divided into odd day group (n = 58, testing group which was treated with vacuum sealing drainage) and even day group (n = 42, control group which was treated with dressing treatment). Length of stay, infection rates and healing were compared between the two groups.
    RESULTS AND CONCLUSION: Length of stay in the testing group was significantly shorter than control group (P < 0.05). In the testing group, two cases had baumanii infection and 4 had pseudomonas aeruginosa infection, and the infection rate was 10%. In the control group, 7 cases had pseudomonas aeruginosa infection and 5 had MRSA infection, and the infection rate was 29%. The infection rate in the testing group was significantly lower than control group (P < 0.05). In the testing group, 46 patients (79%) were cured in the first therapy cycle, and the other 12 cases (21%) were treated with autoallergic free skin transplantation; in the control group, 12 cases were cured in the first therapy cycle, and the other 30 patients were treated with autoallergic skin transplantation following granulation tissue growing. The secondary operation time in the testing group was significantly shorter than control group (P < 0.05). The results suggested that the effect of vacuum sealing drainage technology and absorption materials application were satisfactory in a large area of skin avulsion.

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    Effect of different filling materials on percutaneous vertebroplasty and the image characteristics
    Zhao Gang, Liu Hong-jian, Zhou Ying-jie
    2010, 14 (16):  3007-3010.  doi: 10.3969/j.issn.1673-8225.2010.16.039
    Abstract ( 69 )   PDF (529KB) ( 363 )   Save

    BACKGROUND: Now the filling material which is used for the treatment of osteoporotic compression fractures is mainly polymethacrylic acid (PMMA), but the PMMA as a permanent foreign body cannot promote bone regeneration, and has a downward extension of the possibility of mechanical properties with time prolonging, so a new type of filling material used to substitute PMMA is looking.
    OBJECTIVE: To investigate the effect and image characteristics of different filling materials on percutaneous vertebroplasty performing on a goat model of osteoporotic compression fracture.
    METHODS: Three vertebral fracture models were established on osteoporosis goats by using an appropriate mechanical stress. Under the G-arm X-ray machine, the fractured vertebrae was performed with percutaneous vertebroplasty and then filled with polymethyl methacrylate (PMMA), self-setting calcium phosphate cement (CPC) and recombinant human bone morphogenetic protein-2 (rhBMP-2) /self-calcium phosphate cement.
    RESULTS AND CONCLUSION:  ① A total of 36 vertebral bodies of 12 goats were successfully performed with percutaneous vertebroplasty, and there was no significant difference in filling quantity among three materials (3.5±1.8) mL (P > 0.05). Bolus resistance of PMMA was strong, but operative time was short, and the image was great; the bolus resistance of self-CPC and rhBMP-2/self-CPC was weak, operative time was long, but there was no leakage placeholde. ② CT scanning results on the 2nd day after surgery demonstrated that the three materials were disorderly distributed in the vertebral body, showing high-density patching shaping. Six out of 36 vertebral bodies were leakage, which was induced by PMMA (n = 4), self-CPC (n = 1), and rhBMP-2/self-CPC (n = 1). ③ Scanning electron microscope indicated that all materials in the PMMA group were not able to closely bind with host bone, but self-CPC and rhBMP-2/self-CPC were able to closely bind with host bone. The results suggested that rhBMP-2/self-CPC is characterized by long-term operation, short time of bone substitute, and stabilization of reconstructed spine.

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    Repair of acute anterior cruciate ligament injury using polyethylene terephthalates LARS artificial ligament in 23 cases
    Zhang Jian-lin, Ye Jun, Zhao Jun-hua, Luan Yan-jun, Bai Li-bang
    2010, 14 (16):  3011-3014.  doi: 10.3969/j.issn.1673-8225.2010.16.040
    Abstract ( 66 )   PDF (579KB) ( 424 )   Save

    BACKGROUND: LARS artificial ligament was designed by Laboureau from France using polyethylene terephthalates with the anatomic structure of human ligament and mechanical principle of weight. This ligament is not only accorded with physiological structure of normal anterior cruciate ligament, but also significantly elevates anti-torque, can resist repetitive twist, bend and excessive traction.
    OBJECTIVE: To summarize characteristics of clinical application of LARS artificial ligament in transplant reconstruction following acute anterior cruciate ligament of knee joint injury.
    METHODS: A total of 23 patients with acute anterior cruciate ligament injury, comprising 17 males and 6 females, aged 21-54 years, were selected. Time from injury to surgery was 3 days to 3 weeks. Polyethylene terephthalates LARS artificial ligament made in France was used to reconstruct damaged ligament. The transplant was evaluated before and after implantation according to Lysholm knee joint criteria.
    RESULTS AND CONCLUSION: Following 11.2 months (10-14 months) of follow-up, unstable symptoms of affected knees of all 23 cases disappeared. Anterior and posterior drawer tests were negative, with good joint function. Average extension and flexion degree was 0°-120°. In accordance with Lysholm knee joint score, significant difference was found from (40.34 ± 4.00) points preoperatively to (90.21 ± 4.00) points postoperatively (P < 0.01). They could do common athletic activities at about 2 months following surgery. In some cases, magnetic resonance examination demonstrated that residue ligament gradually grew into artificial ligament, which gradually became thicker. Above-mentioned results have verified that under an arthroscope, reconstruction of anterior cruciate ligament using polyethylene terephthalates LARS artificial ligament showed simple operation and small wound, which may lead to immediate stability, early rehabilitation exercises. Simultaneously, interlacing of residue ligament and artificial ligament keeps the nerve conduction pathway of proprioceptive sense, which prevents joint function limitation greatly. Moreover, short-term outcomes are satisfactory.

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    Heterogeneous acellular dermal matrix patch for repair of oral mucosal defects in 71 patients
    Xue Ling-fa, Shang Wei, Feng Yuan-yong, Jin Xiao-ming, Liu Feng-tong, Jia Mu-yun, Yuan Rong-tao, Bu Ling-xue
    2010, 14 (16):  3015-3018.  doi: 10.3969/j.issn.1673-8225.2010.16.041
    Abstract ( 139 )   PDF (266KB) ( 345 )   Save

    BACKGROUND: Recently, acellular dermal matrix allograft has been widely used in the repair of oral mucosal defects. But little information is about the heterogeneous acellular dermal matrix (HADM) patch for repair of oral mucosal defects.
    OBJECTIVE: To investigate the efficacy and biosafety of HADM in the repair of oral mucosal defects.
    METHODS: In total 71 patients with oral benign or malignant tumors who had oral mucosal or soft tissue defects following tumorectomy were included in this study. These patients comprised 37 males and 34 females, and were averaged 45 years (range, 20-70 years old). Of them, 42 suffered from benign tumors and 29 from malignant tumors. HADM patches were used for repair of oral mucosal defects. The survival, color, and texture of HADM patches were observed. Shrinkage rate of HADM patches was compared between regions without supports from hard tissues (cheeks, tongue, and mouth floor) and with supports from hard tissues (gingiva, hard palate).
    RESULTS AND CONCLUSION: All 71 HADM completely survived. No necrosis and infection occurred. At 2 weeks after transplantation, (98.20±5.20) % of patch area survived. At 3 months after transplantation, patches showed similar color to surrounding oral mucosa and most patients had sense of tension to different extents. At 6 months after transplantation, cell creeping substitution and vascularization were successfully accomplished in the region of patch transplantation. Patches grew stably, with smooth pink appearance and good elasticity, and no further shrinkage. Patients felt normal. HADM patch shrank primarily at 2 weeks-1 month after transplantation, and tended to be stable at 3 months. There was no significant difference in tissue morphology between surgical region and normal tissue. The HADM shrinkage rate was significantly higher in regions without supports from hard tissues than regions with supports from hard tissues. These findings indicate that HADM patches have advantages in repair of oral mucosal defects including good histocompatibility, wide source, simple manipulation, and able to cover the wound surface in the early state, promote wound surface healing, and reduce scar formation, and can be used as an ideal material for repair of oral mucosal defects. 

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    Glucose-lowering effects of insulin delivered by oral inhalation in normal rats
    Wang Zheng-mei, Gao Gui-zhi, Ma Yan
    2010, 14 (16):  3019-3022.  doi: 10.3969/j.issn.1673-8225.2010.16.042
    Abstract ( 122 )   PDF (304KB) ( 372 )   Save

    BACKGROUND: Insulin taken orally is easy to be degraded by hydrochloric acid in the gastric juice and various enzymes in the gastrointestinal tract. It is hard for oral insulin to pass through the epithelial cell membrane in the gastrointestinal tract. Insulin delivered by injection needs at least 36 hours every administration. Many pulmonary administrations of insulin have been recently studied, such as dry powder inhalation, aerosol inhalation, electron spraying, insulin nanometer technique, and absorption enhancer, with promising clinical application prospects.
    OBJECTIVE: To investigate the glucose-lowering effects of insulin delivered by oral inhalation in normal rats.
    METHODS: Thirty healthy male rats, weighing (220±30) g were randomly divided into seven groups: low dose insulin (1.0 U/kg,  n = 4), middle dose insulin (5.0 U/kg, n = 4), high dose insulin (10.0 U/kg, n = 4), insulin (2.0 U/kg) +5% lecithin (n = 5), insulin   (2.0 U/kg) +1% oleic acid (n = 5), insulin (2.0 U/kg) +1% diethylenetriaminepentaacetic acid (DTPA; n = 5), and blank control     (n = 3). At 0, 30, 60, 120, 180, 240 minutes after administration. 1.5 μL blood was taken from rat tails through the use of ONE TOUCHTM BASICTM PLUS blood glucose detection system for determination of blood glucose level and calculation of insulin pharmacological bioavailability under various conditions.
    RESULTS AND CONCLUSION: Insulin (1U/kg) markedly decreased glucose levels immediately after inhalation. Blood glucose level decreased with increasing insulin dose. Insulin (10 U/kg) could produce a 14.5% decrease of blood glucose level. Insulin   (1 U/kg) could yield a pharmacological bioavailability of 11.5% in the absence of absorption enhancer. The concomitant administration of oleic acid, DTPA and lecithin appeared to be more effective in enhancing the pulmonary absorption of insulin, and decreasing blood glucose level.

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    Modified heterogeneous deproteinizated bone composited with recombinant human bone morphogenetic protein for repair of large segmental long bone defects in goat tibia
    Ding Yi, Jian Yue-kui, Li Bo, Zhao Zhu-chuan
    2010, 14 (16):  3023-3027.  doi: 10.3969/j.issn.1673-8225.2010.16.043
    Abstract ( 73 )   PDF (509KB) ( 331 )   Save

    BACKGROUND: For its extensive sources and specific biological characteristics, heterogeneous deproteinized bone (DPB) as the tissue-engineered scaffold material gets a good application prospect in repair of large segmental bone defects.
    OBJECTIVE: To prepare scaffold material by compositing modified heterogeneous DPB with recombinant human bone morphogenetic protein 2 (rhBMP2) and evaluate the osteogenic ability in repairing large segmental long bone defects in large-sized animals.
    METHODS: Twenty-four goats were randomly and evenly divided into three groups: DPB, autologous bone, and DPB+ rhBMP2. Bone defects (20% in length) were made in the middle and lower segments of the right tibia of each goat, and according to group design, different bone grafts were implanted and fixed using semi-ring slot external fixer. At 4-24 weeks after surgery, X-ray examination was performed once every other 4 weeks. At 24 weeks after surgery, newly formed bones were taken out for determining the repair effects of bone defects through dual-energy X-ray analysis, histological observation, and biomechanical analysis.
    RESULTS AND CONCLUSION: The prepared DPB exhibited a three-dimensional porous structure, with a porosity of (78.5±6.45)% and pore size of (472.5±7.02) μm. Time-dependent bone repair was found in each group. Results regarding anti-compression test, three-point anti-bend test, and anti-torsion test were greatest in the autologous bone group, followed by the DPB+ rhBMP2 group, and lastly the DPB group. There were no significant differences in tibial bone density, bone mineral content, anti-compression pressure and ultimate pressure, anti-bending load and ultimate load, anti-torsion torque and ultimate torque between autologous bone group, DPB+ rhBMP2 group and normal bone control group. These findings indicate that modified heterogeneous DPB composited with rhBMP2 has osteogenic ability equivalent to autologous bone in repairing large segmental long bone defects in goat tibias. Therefore, it can be applied as tissue-engineered scaffold material.

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    Construction of tissue engineered cartilage in vivo with poly(lactide-co-glycolic acid) composited with collagen II and growth factors
    Xiong Gao-xin, Zha Zhen-gang, Tan Wen-cheng, Wu Hao, Li Jie-ruo, Lin Hong-sheng,Xia Ji-sheng, Huang Xin-pei, Tu Mei, Zhang Jia-qing
    2010, 14 (16):  3028-3032.  doi: 10.3969/j.issn.1673-8225.2010.16.044
    Abstract ( 78 )   PDF (404KB) ( 392 )   Save

    BACKGROUND: The development of cartilage tissue engineering provides novel ideas for treatment of articular cartilage defects and implements construction of tissue-engineered cartilage in vivo.
    OBJECTIVE: To investigate the feasibility of constructing tissue-engineered osteochondral composite through bone marrow stem cells(BMSCs) cultured on the poly(lactide-co-glycolic acid) (PLGA), which was modified with collagen and cellular growth factors.

    METHODS: PLGA was made by phase separation technique, composited with collagen II, basic fibroblast growth factor, and transforming growth factor-β1. The BMSCs of passage 3 were cultured on the above scaffolds. Thirty-six SD rats were randomly divided into experimental, control, and blank groups. These three groups received implantation of BMSCs composited with growth factors and collagen-PLGA, implantation of BMSCs composited with collagen-PLGA, and implantation of collagen-PLGA into the muscle, respectively. At 4, 8, and 12 weeks after surgery, cell directional differentiation and growth were examined by gross observation, hematoxylin-eosin staining, toluidine blue staining, collagen II staining, and scanning electron microscope.
    RESULTS AND CONCLUSION: Gross observation showed that there were many chondroid tissues in the experimental group and fibrous tissues in the control and black groups. Stainings and electron microscope revealed that many chondroblasts and a few osteoclasts appeared in the composite of the experimental group. Toluidine blue and collagen II stainings were positive in the experimental group and negative in the control and blank groups. These findings demonstrate that PLGA modified with collagen had a good cellular compatibility. BMSCs cultured on PLGA, which was modified with collagen and cellular growth factors, can construct the tissue-engineered osteochondral composite in rats.

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    In vitro characterization of bone cell activity on triphasic ceramic composites from calcium silicate, beta-tricalcium phosphate and hydroxyapatite
    Anne Bernhardt●1, Anja Lode●1, Sivadasan Suresh Babu●2, Antje Vogel, Thomas Hanke, Sebastian Thieme, Hare Krishna Varma, Angela Rösen-Wolff, Michael Gelinsky, Annie John
    2010, 14 (16):  3033-3040.  doi: 10.3969/j.issn.1673-8225.2010.16.045
    Abstract ( 56 )   PDF (642KB) ( 751 )   Save

    Hydroxyapatite (HA) ceramics have been deeply studied over the last decades for application in bone replacement. Due to its close chemical similarity to bone mineral, synthetic HA has been extensively used in clinical applications by virtue of its high biocompatibility and osteoconductivity but shows poor resorbability. We developed a triphasic ceramic composite comprising of HA, beta-tricalcium phosphate (beta-TCP) and calcium silicate. In vitro studies applying human bone marrow-derived mesenchymal stem cells (hMSC) were performed on three different types of triphasic ceramic composites, containing varying weight ratios of HA and beta-TCP/calcium silicate. Cells were cultivated over 28 days with and without osteogenic supplements. To study remodelling, a preliminary experiment for co-culture of osteoblasts and osteoclasts was performed on ceramic composite containing 80% HA. Rat calvarial osteoblasts were cultured together with human monocytes which can be differentiated into osteoclasts. Results showed that hMSC exhibited rapid adhesion and high proliferation rates on the ceramic composite materials. Osteogenic induced hMSC showed an increase of alkaline phosphatase activity on all examined materials. Transcription of genes coding for alkaline phosphatase and bone sialoprotein II was also increased for all three materials. Scanning electron microscopy and confocal laser scanning microscopy investigations demonstrated that osteoclast-like cells were developed from human monocytes on ceramics containing 80% HA. These findings indicate that the novel prepared triphasic ceramic composites support adhesion, proliferation and osteogenic differentiation of hMSC as well as osteoclastogenesis of human monocytes in co-culture with osteoblasts.

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