Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (16): 3028-3032.doi: 10.3969/j.issn.1673-8225.2010.16.044

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Construction of tissue engineered cartilage in vivo with poly(lactide-co-glycolic acid) composited with collagen II and growth factors

Xiong Gao-xin1, Zha Zhen-gang1, Tan Wen-cheng2, Wu Hao1, Li Jie-ruo1, Lin Hong-sheng1,Xia Ji-sheng△3, Huang Xin-pei2, Tu Mei4, Zhang Jia-qing5   

  1. 1 Department of Orthopedics, First Hospital, Jinan University, Guangzhou   510630, Guangdong Province, China; 2 Renhe Medical Center, Macau   999078, China;  3 Hospital of Centre S.Januario, Macau   999078, China; 4 College of Science and Engineering at Jinan University, Guangzhou   510630, Guangdong Province, China;  5 Medical College of Jinan University, Guangzhou   510630, Guangdong Province, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Zha Zhen-gang, Professor, Chief physician, Doctoral Supervisor, Department of Orthopedics, First Hospital, Jinan University, Guangzhou 510630, Guangdong Province, China
  • About author:Xiong Gao-xin★, Studying for master’s degree, Department of Orthopedics, First Hospital, Jinan University, Guangzhou 510630, Guangdong Province, China jiujiu12345678@sina.com
  • Supported by:

    National High-Tech Research and Development Program of China, No.2007AA09Z440*; the Science and Technology Development Fund of Macau of China, No.031/2009/A*; Science and Technology Plan Program of Guangzhou City, No. 2008Z1-E411*

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Abstract:

BACKGROUND: The development of cartilage tissue engineering provides novel ideas for treatment of articular cartilage defects and implements construction of tissue-engineered cartilage in vivo.
OBJECTIVE: To investigate the feasibility of constructing tissue-engineered osteochondral composite through bone marrow stem cells(BMSCs) cultured on the poly(lactide-co-glycolic acid) (PLGA), which was modified with collagen and cellular growth factors.

METHODS: PLGA was made by phase separation technique, composited with collagen II, basic fibroblast growth factor, and transforming growth factor-β1. The BMSCs of passage 3 were cultured on the above scaffolds. Thirty-six SD rats were randomly divided into experimental, control, and blank groups. These three groups received implantation of BMSCs composited with growth factors and collagen-PLGA, implantation of BMSCs composited with collagen-PLGA, and implantation of collagen-PLGA into the muscle, respectively. At 4, 8, and 12 weeks after surgery, cell directional differentiation and growth were examined by gross observation, hematoxylin-eosin staining, toluidine blue staining, collagen II staining, and scanning electron microscope.
RESULTS AND CONCLUSION: Gross observation showed that there were many chondroid tissues in the experimental group and fibrous tissues in the control and black groups. Stainings and electron microscope revealed that many chondroblasts and a few osteoclasts appeared in the composite of the experimental group. Toluidine blue and collagen II stainings were positive in the experimental group and negative in the control and blank groups. These findings demonstrate that PLGA modified with collagen had a good cellular compatibility. BMSCs cultured on PLGA, which was modified with collagen and cellular growth factors, can construct the tissue-engineered osteochondral composite in rats.

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