Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (7): 1141-1145.doi: 10.3969/j.issn.1673-8225.2010.07.001

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Bone regeneration and maturation in a rabbit tibia lengthening model under low-intensity purse ultrasound stimulation

Zhou Jia-hui 1,2, Lü Hong-bin1, Hu Jian-zhong1, Yang Ying1, Zeng Chi1, Qin Ling△3   

  1. 1 Deparment of Sports Medicine, Xiangya Hospital, Central South University, Changsha  410078, Hunan Province, China; 2 the Third Xiangya Hospital of Central South University, Changsha  410078, Hunan Province, China; 3 Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Lü Hong-bin, Doctoral supervisor, Deparment of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410078, Hunan Province, China
  • About author:Zhou Jia-hui★, Master, Physician, Deparment of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410078, Hunan Province, China; the Third Xiangya Hospital of Central South University, Changsha 410078, Hunan Province, China; zjh5518@sina.com
  • Supported by:

    the AO Research Fund of the AO Foundation, No. 05-L21*

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Abstract:

BACKGROUND: Experiments has demonstrated that low-intensity pulse ultrasound stimulations (LIPUS) can accelerate bone healing. However, its affect mechanisms on maturation of regenerated bone remain unclear.
OBJECTIVE: The hypothesis that LIPUS has positive effect on maturation of regenerate bone was put forward creatively, and wants to verify the hypothesis by compare the difference between LIPUS intervention and no intervention.
METHODS: Thirty-six adult, healthy, New Zealand White rabbits were randomly assigned to LIPUS treatment group and control group, with 18 animals in each group. All animals were undergone mid-diaphyseal tibia osteotomy and were immobilized in Orthofix M103 Mini lengtheners. After a seven-day latency period, gradual distraction of 0.5 mm per twelve hours for 10 days to produce 10 mm distraction. A 4-weeks course of LIPUS treatment was applied over the distraction site for twenty minutes daily starting immediately after the completion of distraction. At weeks 4, 8, 12 after distraction, the bone healing was evaluated by X-ray film, and total mineralized areas were measured by Image J software. The newly formed bone in the lengthening field was harvested and stained with haematoxylin-eosin, Masson’s trichrome and VG. The areas of newly formed bone were measured to calculate a percentage of the total callus area.
RESULTS AND CONCLUSION: Thirty-six rabbits were included in the final analysis. At week 4 after distraction, the maturation of newly formed bone in LIPUS treatment group appeared earlier than that in the control groups. The bone callus image presented in the marginal and center region of experimental defect, increased greatly and had higher density until filled of the lengthening fields. Many bone-like masses appeared in the newly formed tissues and the tissues surrounding the bone granules partially changed into bone-like tissues, and the rate of callus production was increased (P < 0.05). There was no significant difference between two groups at weeks 8 and 12 after distraction. Histological results showed that that new bone formation of the LIPUS group was earlier than the control group at weeks 4, 8, and 12 after distraction. The percentage of new bone on total callus area of the LIPUS group was greater than that of the control group at week 4, but there was no obvious difference at weeks 8 and 12 after distraction. LIPUS accelerates new bone formation, increases the size of the distraction callus, exhibits highly effective in achieving maturation of bone in the animal model with distraction osteogenesis.

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