Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (2): 272-28-75.doi: 10.3969/j.issn.1673-8225.2010.02.019

Previous Articles     Next Articles

Establishment of a rat midpalatal suture expansion model

Guo Jing1, Zhang Qi-feng2, Li Gui-feng1, Liu Ze-ping1, Zhou Jing1, Zou Shu-juan1   

  1. 1 State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu   610041, Sichuan Province, China; 2 Department of Stomatology, The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou   310003, Zhejiang Province, China
  • Online:2010-01-08 Published:2010-01-08
  • Contact: Zou Shu-juan, Doctor, Professor, Doctoral supervisor, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Guo Jing☆, Studying for doctorate, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China Tracyguo821218@163.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30772454*; Tackle Key Program of Sichuan Provincial Science and Technology Bureau, No. 2006z09-013*

PDF

330

Abstract:

BACKGROUND: Monkeys, dogs, pigs, rabbits and other large animals have been applied previously to prepare animal models of midpalatal suture expansion, but there are high cost, small sample size, difficult to obtain antibodies and other disadvantages. Wistar rats have wide heads to facilitate cavity operation, with low cost and high reproduction rate, as the midpalatal suture model, it is possible to overcome the above deficiencies.
OBJECTIVE: To establish a rat model of midpalatal suture expansion, and to supply basement for further relative researches of animal models.
METHODS: Twenty Wistar male rats of 5 weeks old, average weight of 65 g, were randomly divided into 2 groups, a experiment and a control, with 10 rats in each group. Rats in experimental group were placed on the expansion appliance, inserting into the diastema between the first and second molars, then stick to molar lingual using light-cured resin for retention. The rats in control group were sham operated, followed by one week of active expansion. After expansion, the midpalatal sections were observed by X-ray and light microscopy.
RESULTS AND CONCLUSION: Maxillary X-ray film showed that midpalatal suture in experimental group was significantly widened, molar lead to cheek. Observed by light microscopy, partial oral side of midpalatal suture in the experimental group was obviously enlarged, mesenchymal cells were spindle, in the same direction to tension force. Below it, traumatic inflammatory response appeared, with a clear bleeding area. The midpalatal suture expansion model in rats is available, simple and reproducible.

CLC Number: