Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (3): 555-558.doi: 10.3969/j.issn.1673-8225.2010.03.043

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Complex of dog allogenic decalcified bone matrix and bone marrow stromal cell sheets
In vitro culture and observation

Tan Shuai, Jing Heng, Gao Zhen-hua, Li Ning-yi   

  1. Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao   266003, Shandong Province, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Li Ning-yi, Professor, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China ningyili342@163.com
  • About author:Tan Shuai★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China tan_shuai@hotmail.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30872896*; the Natural Science Foundation of Shandong Province, No. Y2008C77*

Abstract:

BACKGROUND: How to reconstruct tissue-engineered bone with structure similar to natural bone is a problem in the development of tissue engineering. Cell sheet engineering technology enables novel approaches to construction of tissue-engineered bone.
OBJECTIVE: To observe the biocompatibility of cell sheets to decalcified bone matrix (DBM) and their growth on DBM.
DESIGN, TIME AND SETTING: An in vitro observation was performed at the Central Laboratory, Affiliated Hospital, Qingdao University Medical College between June and September 2009.
MATERIALS: Dog bone marrow stromal cell sheets were prepared using temperature-responsive medium. Dog DBM was prepared by defatting, decalcification, and noncollagen protein removal procedures.
METHODS: DBM surface was covered by cell sheets prepared by temperature-responsive technology and cultured with DMEM containing 10% fetal bovine serum and osteoinductive agent.
MAIN OUTCOME MEASURES: Under scanning electron microscope, DBM structure, as well as the attachment and growth of cell sheets on DBM surface, was observed. Porosity and aperture size of DBM were calculated.
RESULTS: DBM exhibited a three-dimensional latticed structure, with a porosity of approximately 75%. The mean aperture size was (250.11±98.89) µm, exhibiting a normal distribution. Cell sheets well attached to and grew on DBM surface, and rapidly proliferated.
CONCLUSION: Cell sheets show good biocompatibility to DBM. DBM/cell sheets complex can be applied in tissue-engineered bones, which promotes the construction of tissue-engineered bone with structure similar to natural bone.

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