Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (3): 447-451.doi: 10.3969/j.issn.1673-8225.2010.03.016

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Toxicity and biocompatibility of polyethylene glycol-polyethyleneimine/ferroso-ferric oxide nano-magnetic fluid

Zou Fen1, Pan Yi-feng2, Zhang Hong2, Li Guo-zheng2, Tang Jing-bo2, Peng Jian2   

  1. 1 Modern Chinese Medicine Park in South China Co., Ltd, Zhongshan  528451, Guangdong Province, China; 2 National Key Laboratory of Nanobiological Technology, South China University, Changsha  410008, Hunan Province, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Peng Jian, Doctor, Associate professor, Master’s supervisor, National Key Laboratory of Nanobiological Technology, South China University, Changsha 410008, Hunan Province, China
  • About author:Zou Fen★, Master, Modern Chinese Medicine Park in South China Co., Ltd, Zhongshan 528451, Guangdong Province, China miffysweet@126.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30672047*; Postgraduate Innovation Fund of Hunan Province, No. 2340-74335000013*

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Abstract:

BACKGROUND: Polyethylene glycol-polyethyleneimine/ferroso-ferric oxide (PEG-PEI/Fe3O4) was selected as drug carriers in tumor treatment, which can increase drug loading capacity and targeting capacity.
OBJECTIVE: To test the toxicity of PEG-PEI/Fe3O4 nano-magnetic fluid in vitro and in vivo.
METHODS: When the prepared PEG-PEI/Fe3O4 nano-magnetic fluid reached nano level, 7702 and HpG2 cell lines were filtrated and diluted in 5-20 multiple, and detected by in vitro MTT toxicity test assay; in vivo hemolysis test and micronucleus test was used to test the toxicity and biocompatibility.
RESULTS AND CONCLUSION: MTT assay results indicated that the toxicity grade of PEG-PEI/Fe3O4 nano-magnetic fluid to 7702 cell line was 0-1, which was harmless to natural hepatic cells; however, PEG-PEI/Fe3O4 nano-magnetic fluid had slight bystander restraining effect to HpG2 cell line. Maximum hemolysis rate of the material was 0.372%, which was far less than 5%. The micronucleus test result indicated that PEG-PEI/Fe3O4 nano-magnetic fluid had no teratogenicity or mutagenicity.

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