Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (3): 438-442.doi: 10.3969/j.issn.1673-8225.2010.03.014

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Effect of heparin slow-release stent implantation combined with myocardium drilling on myocardial regeneration of pigs with acute myocardial infarction

Zhang Guang-wei1, Liu Xiao-cheng1, Shi Rong-fang1, Zhao Xiao-bin1, Liu Tian-jun2, Lü Feng2   

  1. 1 Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin Medical University, Tianjin  300457, China; 2 Molecular Design Laboratory, Institute of Biomedical Engineering, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300193, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Liu Xiao-cheng, Doctor, Professor, Chief physician, Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin Medical University, Tianjin 300457, China TEDA_LXC@163.com
  • About author:Zhang Guang-wei☆, Doctor, Physician, Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin Medical University, Tianjin 300457, China lion-roar@163.com
  • Supported by:

     the Tianjin Foundation for Development of Science and Technology, No. 05YFGZSF02900*

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Abstract:

BACKGROUND: A new method, i.e., heparin slow-release stent implantation combined with myocardium drilling, is discovered for myocardial revascularization, which remarkably improves myocardial perfusion.
OBJECTIVE: To investigate the effect of heparin slow-release stent implantation combined with myocardium drilling on myocardial regeneration of pigs with acute myocardial infarction.
METHODS: Anterior descending coronary of pig was ligated to induce myocardial infarction model, which was randomly divided into control and implantation groups, with 6 pigs for each group. Self-made borer was used in the implantation group to drill two transmural channels (3.5 mm diameter) on epicardium. A heparin slow-release stent was fixed in the transmural channel. Following intravenous injection, BrdU was used to label DNA duplication so as to observe stromal cell-derived factor-1 (SDF-1) mRNA expression, myocardial perfusion, newborn yocardium, and heart function prior to and following implantation.
RESULTS AND CONCLUSION: As compared with control group, SDF-1 expression was enhanced in the implantation group at 6 weeks after stent implantation (P < 0.001), perfusion mass defect percentage was significantly decreased (P < 0.001), ejection fraction of left ventricle was increased (P < 0.05), newborn myocardium was increased (P < 0.001), and survival myocardium in the ischemic region was increased (P < 0.001). The results demonstrated that heparin slow-release stent implantation combined with myocardium drilling could repair damaged myocardial cells and improve heart function through increasing SDF-1 expression and myocardial perfusion.

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