Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (3): 429-432.doi: 10.3969/j.issn.1673-8225.2010.03.012

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Effect of chitooligosaccharides on cyclin D1, bcl-xl and bcl-2 mRNA expression in Hela cells

Li Xian1, Wang Ju2, Zhou Chang-ren1, Tian Jin-huan1, Chen Xiao-jia2, Zhao Ming-yan1   

  1. 1 Department of Materials Science and Engineering, School of Technology, Jinan University, Guangzhou  510632, Guangdong Province, China; 2 Bio-engineering Institute of Jinan University, Guangzhou     510632, Guangdong Province, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Zhou Chang-ren, Professor, Doctoral supervisor, Department of Materials Science and Engineering, School of Technology, Jinan University, Guangzhou 510632, Guangdong Province, China tcrz9@jnu.edu.cn
  • About author:Li Xian☆, Studying for doctorate, Department of Materials Science and Engineering, School of Technology, Jinan University, Guangzhou 510632, Guangdong Province, China lxchina2006@163.com
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2007AA091603*

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Abstract:

BACKGROUND: Studies have shown that chitooligosaccharides have antitumor effect. However, the influence of chitooligosaccharides on cyclin D1, bcl-2 and bcl-xl remains unclear.
OBJECTIVE: To observe the inhibition effect of chitooligosaccharides on the proliferation of Hela cells, and the influence on cyclin D1, bcl-xl and bcl-2 mRNA expression.
METHODS: Hela cells were stimulated by different concentrations of chitooligosaccharides (0.1, 0.5, 1, 2, 5 g/L). The effects of chitooligosaccharides on Hela cells were detected by CCK8 kit. Using real-time PCR methods, the gene expression of cyclin D1, bcl-xl and bcl-2 mRNA were determined.
RESULTS AND CONCLUSION: Chitooligosaccharides inhibited the proliferation of Hela cells. With the concentrations of chitooligosaccharides increased from 0.1 g/L to 2 g/L, the inhibition effects on the gene expression of cyclin D1, bcl-xl and bcl-2 were enhanced, peaked at 2 g/L, and decreased at high concentration (5 g/L). Antitumor activity of chitooligosaccharides may exert through two aspects: it inhibits cyclin D1 mRNA expression to suppress the proliferation of tumor cells; on the other hand, chitooligosaccharides inhibits the expression of bcl-xl and bcl-2 to promote the apoptosis of tumor cells. Moreover, the effects of the former are stronger than the latter.

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