Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (3): 424-428.doi: 10.3969/j.issn.1673-8225.2010.03.011

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Sulfonation of polyethersulfone sheets effects on adsorbability of beta 2-microglobulin: Whether the adsorbability changes with increased sulfonation degree?

Ma Xing-yu1, Sun Xiao-qing2, Cheng Li-ping3, Sun Shu-dong1, Yue Yi-lun1, Huang Jia1, Mao Hua-yi4   

  1. 1 Department of Biomaterials and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu  　610065, Sichuan Province, China; 2 Department of Special Laboratory, Renshou People’s Hospital, Renshou  620500, Sichuan Province, China; 3 College of Material Science and Engineering, Sichuan University, Chengdu  610065, Sichuan Province, China; 4 Nuclear Power Institute of China, Chengdu  610041, Sichuan Province, China　
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Sun Shu-dong, Doctor, Associate professor, Department of Biomaterials and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu 　610065, Sichuan Province, China
  • About author:Ma Xing-yu★, Studying for master’s degree, Department of Biomaterials and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu 　610065, Sichuan Province, China onlyonly_0@tom.com

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Abstract:

BACKGROUND: Dialysis-related amyloid may occur during long-term dialysis for patients with uraemia, of which the main evocator is β2 -microglobulin (β2 M); therefore, how to eliminate β2M from blood is always the focus of research.
OBJECTIVE: To observe ability of removal of β2 -microglobulin (β2M) from serum using two kinds of polyethersulfone (PES) membrane materials with various degrees of sulfonation.
METHODS: These materials were incubated in radio-labeled β2 M (125 I-β2M) solution and human serum respectively at appointed time at 37 ℃, and then the amounts of 125 I-β2M and serumβ2M adsorbed by materials were measured by radio immunoassay.
RESULTS AND CONCLUSION: In the 125 I-β2M system, amounts of 125 I-β2M adsorbed by the materials decreased in the following sequence PES with high degree of sulfonation > PES with low degree of sulfonation > PES, whatever the source of PES was. In the serum system, amounts of β2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES with high degree of sulfonation > PES with low degree of sulfonation > PES. Sulfonated PES removed β2M more than PES did and the adsorption of β2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of β2M may result in less β2M available for incorporation into amyloid. The use of sulfonated PES membranes may lessen the likelihood of development of dialysis-related amyloidosis, which remains a major source of morbidity for patients treated with long-term hemodialysis.

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