Effect of massage on extracellular matrix collagen deposition in skeletal muscle of type 2 diabetic rats

doi:10.12307/2025.784

Abstract

Abstract: BACKGROUND: Studies have found that massage can reduce blood sugar, promote myogenic factor expression, and increase skeletal muscle content. The extracellular matrix is an important component of skeletal muscle, and association between massage and extracellular matrix and their mechanism of action are still unclear.
OBJECTIVE: To explore the effect of massage on extracellular matrix collagen deposition in type 2 diabetic sarcopenia rats.
METHODS: Totally 24 Wistar male rats were randomly divided into blank group, model group, and massage group. High-fat diet combined with the streptozotocin method was used to establish a type 2 diabetes mellitus and sarcopenia model. After successful model establishment, the massage group used abdominal massage combined with hind limbs. After 8 weeks of treatment, the fasting blood glucose and serum insulin levels of the rats were measured. The skeletal muscle mass was detected by dual-energy X-ray. The exhaustion time was measured by small animal treadmill. The sliding angle was measured by inclined board test. The pathological changes of skeletal muscle tissue were observed by hematoxylin-eosin staining. The skeletal muscle collagen deposition was observed by Masson staining. The mRNA and protein expressions of type I and type III collagen in skeletal muscle were detected by qPCR and western blot assay.
RESULTS AND CONCLUSION: (1) Compared with the model group, the blood glucose (P < 0.05) and serum insulin (P < 0.01) decreased in the massage group. (2) Compared with the model group, the skeletal muscle mass, running exhaustion time, and the angle of inclined plate experiment were increased in massage group (P < 0.05). (3) Compared with the model group, the skeletal muscles of the massage group were arranged neatly, muscle atrophy was improved, and collagen fiber deposition was reduced. (4) Compared with the model group, the expression levels of type I and type III collagen mRNA and protein in skeletal muscle were decreased in the massage group (P < 0.05). (5) The results suggest that massage can enhance insulin sensitivity, lower blood sugar, improve skeletal muscle mass, strength and function, and diminish collagen deposition in rats with type 2 diabetes, and may be a potential target for massage to exert its therapeutic effects.

Key words: massage, type 2 diabetes, sarcopenia, extracellular matrix, collagen deposition, type I collagen, type III collagen, engineered tissue construction

CLC Number:

Sun Yahui, Wang Yufeng, Guo Chao, Yao Junjie, Ji Yuanyuan, Li Zhongxu, Lou Huijuan, Jiang Jinglei, Sun Yiping, Xu Jing, Cong Deyu. Effect of massage on extracellular matrix collagen deposition in skeletal muscle of type 2 diabetic rats[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(26): 5549-5555.

Figures/Tables 5

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