Screening and optimization of three-dimensional scaffolds for the construction of bioartificial liver bioreactor

doi:10.12307/2021.053

Abstract

Abstract:

BACKGROUND: Bioreactor is the core part of bioartificial liver, and its performance determines the effect of bioartificial liver support therapy. The application of scaffolds in hollow fiber bioreactor is expected to solve the problem of insufficient support for hepatocyte culture.

OBJECTIVE: To screen three-dimensional scaffolds for the development of bioartificial liver reactor.
METHODS: The porosity of polyurethane, chitosan and poly(DL-lactide-co-glycolide) scaffolds was detected. HL-7702 human hepatocytes were seeded on three kinds of scaffolds respectively, and the cell inoculation rate was detected at 4 hours; the cell viability and lactate dehydrogenase leakage were detected at 24 hours; and the intracellular albumin synthesis was detected at 3 days. According to the above experimental results, polyurethane was selected in the following experiment. In the rat tail collagen coating group, material was inoculated with rat tail collagen solution and then inoculated with HL-7702 human hepatocyte suspension. In the fibrin gel group, HL-7702 human hepatocytes were resuspended in fibrinogen culture medium, and then inoculated on the scaffold. In the polyurethane control group, material was inoculated with HL-7702 human hepatocyte suspension directly. After 1, 3, 5, and 7 days of culture, cell viability, lactate dehydrogenase leakage and albumin synthesis were detected.
RESULTS AND CONCLUSION: (1) The three kinds of scaffolds were porous. Polyurethane and chitosan scaffolds had good elasticity, but poly(DL-lactide-co-glycolide) scaffold was hard. The porosity of polyurethane and chitosan scaffolds was significantly higher than that of poly(DL-lactide-co-glycolide) scaffold (P < 0.05). (2) The inoculation rate of cells in polyurethane scaffold was significantly higher than that in poly(DL-lactide-co-glycolide) scaffold (P < 0.05), and significantly lower than that in chitosan scaffold (P < 0.05). The cell viability and albumin synthesis of cells cultured in polyurethane and chitosan scaffolds were significantly higher than that of poly(DL-lactide-co-glycolide) scaffold (P < 0.05). There was no significant difference in lactate dehydrogenase leakage among the three groups (P > 0.05). (3) The cell distribution of fibrin gel group was more homogeneous than that of polyurethane control group and rat tail collagen coating group. The cell viability and albumin content at 3, 5, and 7 days after culture were higher than those of polyurethane control group and rat tail collagen coating group (P < 0.05). The lactate dehydrogenase leakage amount at 3, 5, and 7 days of culture was lower than that of polyurethane control group and rat tail collagen coating group (P < 0.05). (4) Our results suggested that fibrin gel optimized polyurethane scaffolds could be used in the construction of bioartificial liver reactor.

Key words: material, scaffold, three-dimensional scaffold, polyurethane, bioreactor, bioartificial liver, biocompatibility

CLC Number:

Chen Li, Huang Lei, Yu Mengyao, Zhang Guoying, Zhang Shichang. Screening and optimization of three-dimensional scaffolds for the construction of bioartificial liver bioreactor[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(28): 4429-4434.

Figures/Tables 9

References

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