Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (16): 2945-2948.doi: 10.3969/j.issn.1673-8225.2010.16.024

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Research and progress of microcarrier culture technology

Zhou Yan1, Liu Bao-lin1, Yang Bo1, Wang Xin1, Peng Cheng-hong 2,3 , Han Bao-san 2,3   

  1. 1 Institute of Biothermal Engineering, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai  200093, China;2 Department of General Surgery, Diagnostic and Treating Center of Breast Cancer, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai  200025, China; 3 Shanghai Institute of Digestive Surgery, Shanghai 200025, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Han Bao-san, Doctor, Associate chief physician, Department of General Surgery, Diagnostic and Treating Center of Breast Cancer, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China; Shanghai Institute of Digestive Surgery, Shanghai 200025, China hanbaosan@126.com
  • About author:Zhou Yan★, Studying for master’s degree, Institute of Biothermal Engineering, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China zhou8713@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50776060*, 30672043*, 30772105*, 20074031*, 30801107*; Program for New Century Excellent Talents in University, No. 07-0559*; Oriental Scholar Program of Shanghai*; the State Plan for High-Tech Research and Development in Eleventh-five Year (National 863 Program), No. 2008AA02Z417*; Innovation Program of Shanghai Municipal Education Commission, No. 09YZ84*; the Natural Science Foundation of Shanghai Municipal Education Commission, No. 07ZR1407*; Shandong Province Science and Technology Research Program, No. 2008GG10002027*

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Abstract:

BACKGROUND: Microcarrier culture technology has become a new and large scale cell culture technology. It has been mainly used in the amplification research of tissue engineering seed cells. Microcarrier possesses the advantage of larger surface area and plays an essential role in microcarrier culture technology.
OBJECTIVE: To sum up the biomaterials and methods of microcarrier preparation, and provide theoretical foundation for the study of microcarrier culture technology and tissue engineering.
METHODS: Articles were retrieved from PubMed, Wanfang, and VIP databases with the key words of “microcarrier, biomaterials cell culture, tissue engineering” in both English and Chinese between 1967/2009 and 1990/2009, respectively. Inclusion criteria: study addressing microcarrier materials, preparation, and performance; study of microcarrier cell culture; animal experiments and clinical applications. A total of 34 articles were originally retrieved based on their titles and abstracts.
RESULTS AND CONCLUSION: Although a lot of studies have reported research and preparation of microcarrier, clinical application remains still difficult. Recently, varying materials will be made into novel compound materials by new technology, which can adjust mechanics and biodegradation of microcarrier via surface modification.

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