Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (12): 2114-2118.doi: 10.3969/j.issn.1673-8225.2010.12.007

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Biocompatibility of bone cement containing microspheres in vivo

Deng Yong-zhong1, Li Sen2, Jin An-min3, Xue Zhong-lin4, Zhang Hui3　   

  1. 1 Department of Orthopaedics, Fourth Hospital of Chinese PLA, Xining   810007, Qinghai Province, China; 2 Department of Spinal Orthopaedics, Hospital Affiliated to Luzhou Medical College, Luzhou   646000, Sichuan Province, China; 3 Orthopaedics Center, Zhujiang Hospital, Southern Medical University, Guangzhou   510282, Guangdong Province, China; 4 Department of Orthopaedics, Central Hospital, Jiangmen   529000, Guangdong Province, China
  • Online:2010-03-19 Published:2010-03-19
  • Contact: Zhang Hui, Doctor, Associate chief physician, Orthopaedics Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China zhanghuixf@163.com
  • About author:Deng Yong-zhong, Associate chief physician, Department of Orthopaedics, Fourth Hospital of Chinese PLA, Xining 810007, Qinghai Province, China Doctordyzhong@sina.com
  • Supported by:

    the Science and Technology Plan Program of Guangdong Province, No. 2007B03 1003009*

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Abstract:

BACKGROUND: Our previous studies have confirmed that bone cement with polylactic acid-polyglycolic acid copolymer microspheres has a high strength, good injection property and in vitro degradable function, but its biocompatibility is still unclear.
OBJECTIVE: To select acute toxicity test, hemolytic test and micronucleus test, and to determine biocompatibility of bone cement containing microspheres.
METHODS: Columnar bone cement and microsphere-coated bone cement were synthesized. 1 g material was added into    100 mL phosphate buffer and immersed for 3 days under sterile condition to extract supernatant, namely bone cement leaching liquor and microsphere-coated bone cement leaching liquor. The toxicity of the product was detected using acute toxicity testing, hemolysis test, micronucleus test and pyrogen test.
RESULTS AND CONCLUSION: Acute toxicity testing results showed that no significant difference was determined between the bone cement leaching liquor and microsphere-coated bone cement leaching liquor groups (except high concentration of microsphere-coated bone cement group) and negative control group. This indicated that the leaching liquor had no significant toxic reaction in rats. Hemolysis test demonstrated that the hemolysis ratio was less than 5% in each cement group. Micronucleus test results exhibited that significant differences in micronuclear rates were determined between 0.4 mL bone cement and 0.4 mL microsphere-coated bone cement groups and negative control group, but no significant difference was detected among other groups. This revealed that this microsphere-coated bone cement leaching liquor did not show significant cell heredity toxic effects. Nevertheless, microsphere-coated bone cement in the high concentration and high dosage groups exhibited low toxic effects, mainly showing increased hemolysis rate and micronuclear rate. Thus, it was necessary to control the dosage and concentration of microsphere-coated bone cement. Pyrogen test confirmed that the average increased temperature was 0.06 ℃, lower than  1.4 ℃ in rabbits following injection. This shows no induced thermal effects of the material.

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