Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (12): 2106-2110.doi: 10.3969/j.issn.1673-8225.2010.12.005

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Effects of recombinant human bone morphogenetic protein-2 combined with sodium hyaluronate on mandible augmentation

Lai Ren-fa1, Liu Xiang-ning1, Zhao Qing-tong1, Wang Ju2, Gong Jian3, Li Hong1   

  1. 1 Stomatology Center, 3Department of Nuclear Medicine, First Affiliated Hospital of Jinan University, Guangzhou  510630, Guangdong Province, China; 2 Institute of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Online:2010-03-19 Published:2010-03-19
  • About author:Lai Ren-fa☆, Doctor, Chief physician, Stomatology Center, First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China Tlrf@jnu.edu.cn; Prof.Dr.Lai@163.com
  • Supported by:

    Guangdong Science and Technology Development Foundation, No. 2006B35801004*

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Abstract:

BACKGROUND: The solid stent material is unsuitable for patient needs for mandible augmentation. Therefore, it is a research focus to explore a new material which characterized by fluidity, attachment and small volume.  
OBJECTIVE: To evaluate the bone augmentation efficacy of recombinant human bone morphogenetic protein (rhBMP-2) and sodium hyaluronate (SH) composite injection on mandibula of rabbits.
METHODS: According to random block design, 60 rabbits were randomly divided into 4 groups: rhBMP-2-SH, rhBMP-2, SH and blank control groups. 0.2 mL rhBMP-2-SH composites, 0.5 mg rhBMP-2, or 0.2 mL SH were placed beneath both sides of mandible periosteum in the rhBMP-2-SH group, rhBMP-2 and SH groups, respectively. Nothing was placed in control group. rabbits were sacrificed at weeks 2, 4, and 8 after operation. 99Tcm-MDP bone scan was performed before sacrificed. While other exams, including haematoxylin-eosin staining, collagen I immunochemical staining, alkaline phosphatase (ALP) activity, bone gla protein (BGP) content, were performed after sacrificed.
RESULTS AND CONCLUSION:  ① Ratio of region of interest count of the rhBMP-2-SH group was greater than that in other groups at weeks 2 and 8 (P < 0.05), especially significantly higher at week 4 (P < 0.01). ② Histological observation showed that newly formed bone trabeculae were found at 2 weeks, which calcifying into lamellar bone at 8 weeks. Cartilaginous cells were only found at 4 weeks, and strong expression of collagen I was only found in the osteoblast stroma. ③ ALP activity index was significantly higher than that in other groups (P < 0.01), and increased with time prolonged (P < 0.01). ④ BGP content was significantly higher than that in other groups at weeks 4 and 8 (P < 0.01), and increased with time prolonged (P < 0.01). Bone formation and augmentation efficacy of rhBMP-2-SH composite injection on the bone surface of rabbit mandibula were verified. That osteoinductive manner is a kind of mixed ossification, mainly with intramembranous ossification.

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