Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3728-3732.doi: 10.3969/j.issn.1673-8225.2011.20.028

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Total saponins of Panax notoginseng effects on mRNA expression of osteoprotegerin in osteoblasts from rabbits with avascular necrosis of the femoral head

Wang Da-wei, Shi Bao-ming, Li Xiao-feng, Zhang Shuang, Mo Jian, Gu Guo-long, Lin Bao-ju, Ma Kai-hao, Hui Ning-jun   

  1. Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning  530011, Guangxi Zhuang Autonomous Region, China
  • Received:2011-03-10 Revised:2011-03-19 Online:2011-05-14 Published:2011-05-14
  • Contact: Shi Bao-ming, Studying for master’s degree, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China 26826713@qq.com
  • About author:Wang Da-wei, Professor, Chief physician, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China wdw1956@yeah.net
  • Supported by:

    Key Science and Technology Program of Guangxi Health Bureau, No. 200853*

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Abstract:

BACKGROUND: Previous studies have demonstrated that total saponins of Panax notoginseng (PNS) can inhibit the ethanol-induced adipogenic differentiation of rabbit bone marrow stromal cells (BMSCs).
OBJECTIVE: To observe PNS effects on osteoblast proliferation and mRNA expressions of osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL).
METHODS: Rabbit models of alcohol-induced avascular necrosis of the femoral head were divided randomly into saline group, compound bone peptide group, PNS group. Rabbit osteoblasts were extracted for measurement of OPG and RANK mRNA expressions and hybridizing signal intensity.
RESULTS AND CONCLUSION: In the PNS group, alkaline phosphatase activity, calcium nodule count, OPG and RANK mRNA expressions and their ratio were significantly higher than those in the saline group (P < 0.01), and similar to those in the compound bone peptide group. The experiment confirmed that PNS can promote the proliferation and differentiation of rabbit osteoblasts, and can improve the OPG mRNA relative expression in osteoblasts so as to inhibit RANKL mRNA expression.

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