Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3723-3727.doi: 10.3969/j.issn.1673-8225.2011.20.027

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Effects of scutellarin on vascular endothelial growth factor and protein kinase Cε expression in human umbilical vein endothelial cells after ischemia-reperfusion injury

Li Lin1, Liu Hua2, Cai Hong-yan1, Yang Wei-min3, Guo Tao1   

  1. 1Department of Cardiology, 2Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming  650032, Yunnan Province, China;    3Yunnan Pharmacology Lab of Natural Product, Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2011-02-14 Revised:2011-03-19 Online:2011-05-14 Published:2011-05-14
  • Contact: Guo Tao, Master, Professor, Chief physician, Department of Cardiology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China guotao20@hotmail. com Correspondence to: Yang Wei-min, Doctor, Investigator, Yunnan Pharmacology Lab of Natural Product, Kunming Medical University, Kunming 650032, Yunnan Province, China ywmbessie@yeah. net
  • About author:Li Lin☆, Studying for doctorate, Attending physician, Department of Cardiology, 2Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China lilinkm@yahoo.com. cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30960450*

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Abstract:

BACKGROUND: The effects of scutellatin on ischemia-reperfusion injury to endothelial cells in vitro have not been elucidated.
OBJECTIVE: To investigate the effect of scutellatin on human umbilical vein endothelial cells (HUVECs) after ischemia-reperfusion and to study whether this effect is mediated by vascular endothelial growth factor (VEGF) and protein kinase Cε (PKCε).
Methods: Cultured HUVECs, exposed to oxygen and glucose deprivation followed by reperfusion, were used as an in vitro model of ischemia-reperfusion. After incubated with scutellarin in a low (1×10-7 mol/L), middle (1×10-6 mol/L) and high (1×10-5 mol/L) dose for 30 minutes, cells were treated by ischemia 3 hours/reperfusion 5 to 24 hours. VEGF protein expression was evaluated by Western blotting. The translocation of PKCε was assayed by Western blotting.
RESULTS AND CONCLUSION: Ischemia 3 hours/reperfusion 5 hours injury significantly decreased the expression of VEGF, and scutellatin in the high and middle dose significantly increased VEGF expression (P < 0.05). Under ischemia 3 hours/reperfusion 24 hours condition, VEGF expression was not changed and scutellatin failed to further increase the expression of VEGF. Compared with the control group, there were significant increases of PKCε expression in particulate fractions in ischemia-reperfusion group and scutellatin pretreatment groups (P < 0.01). Compared with ischemia-reperfusion group, there was no further increase of PKCε in scutellatin groups. Scutellarin pretreatment on vascular endothelial cells after ischemia-reperfusion injury may have a protective effect, and the mechanism may be related to the early increase of VEGF.

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