Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3645-3648.doi: 10.3969/j.issn.1673-8225.2011.20.009

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Construction of a high intraosseous pressure type rabbit osteoarthritis model

Dai Qi-yi1, Qin Xue-liu1, Yuan Jing-yang1, Lin Guang-qi2, Liu Jing2, Ruan Ping1, Teng Ju-zan1, Rong Xiang-bin1, Han Jie1, Qin Jie1, Wu Zhao-pei1, Wang Xiong1   

  1. 1Department of Orthopedic Surgery and Pathology, Ruikang Affiliated Hospital of Guangxi Traditional Chinese Medical University, Nanning  530011, Guangxi Zhuang Autonomous Region, China
    2Department of Anatomy and Biochemistry, Guangxi Traditional Chinese Medical University, Nanning 530001, the Guangxi Zhuang Autonomous Region, China
  • Received:2010-10-27 Revised:2010-11-20 Online:2011-05-14 Published:2011-05-14
  • Contact: Qin Xue-liu, Associate professor, Department of Orthopedic Surgery and Pathology, Ruikang Affiliated Hospital of Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China qinxueliu2008@163. com
  • About author:Dai Qi-yi★, Master, Professor, Department of Orthopedic Surgery and Pathology, Ruikang Affiliated Hospital of Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China diqy3146423@163. com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860359*; the Natural Science Foundation of Science and Technology Commission of Guangxi Zhuang Autonomous Region, No. 0832178*

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Abstract:

BACKGROUND: Plenty of animal models of knee osteoarthritis has been established and used, most of them may not be right for the experimental study of Chinese medicine manipulation.
OBJECTIVE: To determine the outcomes of constructed animal models of high intraosseous pressure knee osteoarthritis.
METHODS: The inferior gluteal vein, femoral vein and great saphenous vein on the right side were exposed under aseptic conditions, ligatured and cutting off the veins. The animal with exposed vessel and normal animals were served as controls. All animals were sacrificed at 8 weeks after modeling. The structure changes of cartilage tissues were observed by gross observation, haematoxylin-eosin staining, and light microscope.
RESULTS AND CONCLUSION: Articular surface of the model group was rough and dark-black, with obvious defects and osteophytosis, with the early and medial stages of osteoarthritis. Articular surface of sham-surgery group was rough, opacity and grey, with the early stages of osteoarthritis. Articular surface of the normal group was smooth, marginal regular, translucent shine for articular cartilage, with no obvious cartilage degeneration. The results revealed that, at 8 weeks after ligaturing the inferior gluteal vein, femoral vein and great saphenous vein would cause the early and medial stages of osteoarthritis in rabbits.

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