Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3641-3644.doi: 10.3969/j.issn.1673-8225.2011.20.008

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A co-culture model of tissue engineering epidermis and THP-1 cells for sensitization testing

Cao Yu-ping, Zhou Wu-qing, Ma Peng-cheng, Liu Wei-da, Li Ling-jun, Tao Yue, Zhang Meng-li, Tao Lei, Wei Jun   

  1. Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College, Nanjing  210042, Jiangsu Province, China
  • Received:2011-01-06 Revised:2011-04-06 Online:2011-05-14 Published:2011-05-14
  • Contact: Ma Peng-cheng, Investigator, Professor, Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College, Nanjing 210042, Jiangsu Province, China mpc815@163.com Correspondence to: Liu Wei-da, Doctor, Investigator, Professor, Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College, Nanjing 210042, Jiangsu Province, China liumyco@hotmail. com
  • About author:Cao Yu-ping☆, Studying for doctorate, Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College, Nanjing 210042, Jiangsu Province, China cypcherry@yahoo. com.cn
  • Supported by:

    the Natural Science Foundation of Jiangsu Province, No. BK2008092*

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Abstract:

BACKGROUND: Several studies have shown that tissue engineering skin are used for skin irritation testing.
OBJECTIVE: To construct a co-culture model of tissue engineering epidermis and THP-1 cells, and to identify the specificity of the co-culture model to sensitizers.
METHODS: Tissue engineering epidermis was constructed by human keratinocytes. Then, THP-1 cells were co-cultured tissue engineering epidermis. After 24 hours treatment on tissue engineering epidermis, the cultured medium and THP-1 cells were collected, respectively. ELISA was used to detect the concentration of interleukin 1beta (IL-1β) in the culture medium, and the mean fluorescence intensities (MFI) measured by flow cytometry were used to evaluate the expression of CD86 and CD54 on THP-1 cells.
RESULTS AND CONCLUSION: After 14 days keratinocytes were cultured at air-liquid interface, HE staining showed 8-10 layers in tissue engineering epidermis. And pan-keratin immuohistochemistry staining was positive. After 24 hours treatment of chemicals, the concentration of IL-1β and MFIs of CD86 and CD54 in DNFB-treated group were higher that that in vehicle control group (P < 0.05). The results prove that a co-culture model of tissue engineering epidermis and THP-1 cells was successfully established, and it can be used for sensitization test, which gives a new method for toxicity testing in vitro.

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