Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3351-3353.doi: 10.3969/j.issn.1673-8225.2011.18.029

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Establishment of a rat model of ischemic biliary strictures induced by clamping

Zhao De-fang1, Zhang Tong1, Zhao Li-jun2, Chen Da-zhi3   

  1. 1Department of Hepatobiliary Surgery, Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010059, Inner Mongolia Autonomous Region, China
    2Department of Pathophysiology, Medical College, Hebei United University, Tangshan  063000, Hebei Province, China
    3Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Beijing  100021, China
  • Received:2010-12-03 Revised:2011-03-06 Online:2011-04-30 Published:2011-04-30
  • Contact: Chen Da-zhi, Doctor, Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Beijing 100021, China
  • About author:Zhao De-fang☆, Doctor, Associate chief physician, Department of Hepatobiliary Surgery, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China hostmanabc@hotmail.com

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Abstract:

BACKGROUND: Studies have showed that forceps operation can be used to prepare myocardial ischemia-reperfusion injury models.
OBJECTIVE: To prepare a rat biliary ischemic stenosis model using forceps operation.
METHODS: After the optimal time of biliary ischemia was determined, 40 Wistar rats were randomly divided into 2 groups. 0.8 cm common bile duct was clamped for 90 minutes with 2 micro-vessel clips in the experimental group. The common bile duct was not clamped in the sham operation group. Twenty-one days later, the intravenous cholangiography was conducted in all the rats. Blood samples were collected from the inferior vena cava to investigate the serum concentration of total bilerubin (TBIL) and direct bilerubin (DBIL).
RESULTS AND CONCLUSION: Twenty-one days later: 3 rats in experimental group could be evidenced to bile duct distension by intravenous cholangiography. But they all had serious dilatation, and their liver was bad when autopsy was done. Obvious dilatation of the common bile duct appeared over the clamped site. There was no dilatation of the common bile duct in the sham operation group. The serum concentration of TBIL and DBIL were significantly higher in the experimental group than in the sham operation group (P < 0.05). The result proved that The rat model of ischemic biliary strictures resulting from clamping has been successfully established.

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