Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3347-3350.doi: 10.3969/j.issn.1673-8225.2011.18.028

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Rat models of orthotopic kidney transplantation

Mai Hai-xing, Qu Nan, Zhao Li, Huan Chen, Wang Ya-lin, Li Xue-chao, Li Jian-tao, Chen Li-jun   

  1. Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China
  • Received:2010-10-26 Revised:2010-12-02 Online:2011-04-30 Published:2011-04-30
  • Contact: Chen Li-jun, Doctor, Professor, Master’s supervisor, Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China chenlijun829@hotmail.com
  • About author:Mai Hai-xing★, Master, Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China maimark24@hotmail.com

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Abstract:

BACKGROUND: There are many ways to prepare kidney transplantation models in rats; however, there are still many problems about operation time, transplantation effect and so on.
OBJECTIVE: To study the microsurgical technique of establishing a reliable rat model of orthotopic kidney transplantation.
METHODS: Kidney transplantation was performed from SD to Wistar strain (allogeneic),the donor’s artery and renal vein were put on the self-make rubber septum and underwent the end to end anastomosis surgeon with the receptor’s arteriae renalis and renal vein. After that, the donor’s bladder valva was inosculated with the receptor’s bladder. All the rats were divided into two groups: control group and Cyclosporin A (CsA) group, each group included 30 rats. The control group received 1 mL D-hanks each day after transplantation; the CsA group received subcutaneous injection of CsA for 15 mg/kg. The serum creatinine levels were observed at days 3, 5 and 10 after transplantation, pathological changes were also observed at 10 days after transplantation.  
RESULTS AND CONCLUSION: Rat orthotopic kidney transplantation was performed in 60 rats, and the successful rate was 85%. The serum creatinine level in the CsA group was lower than that in the control group (P < 0.05), but the survival time in the CsA group was longer than that in the control group (P < 0.05). Allografts of the control group exhibited typical severe acute rejection. It is indicated that this rat model of kidney transplantation is a reliable model with good reproducibility and high achievement ratio.

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