Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5169-5172.doi: 10.3969/j.issn.1673-8225.2011.28.010

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Effects of insulin-like growth factor 1 on expression of Wnt signaling pathway-related factors in mouse osteoblasts

Guo Ling1, Zheng Li-ge1, Wang Min2, Hao Liang2   

  1. 1Department of Prosthodontics, Stomatology Hospital Affiliated to Luzhou Medical College, Luzhou  646000, Sichuan Province, China
    2Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2011-01-19 Revised:2011-03-25 Online:2011-07-09 Published:2011-07-09
  • Contact: Zheng Li-ge, Professor, Department of Prosthodontics, Stomatology Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China Lzyxy-zlg@163.com
  • About author:Guo Ling☆, Doctor, Lecturer, Department of Prosthodontics, Stomatology Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China glsmiling@yahoo.cn
  • Supported by:

    Foundation of Ministry of Healthy of Sichuan Province, No. 20090201*

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Abstract:

BACKGROUND: Insulin-like growth factor 1 (IGF-1) plays a crucial role in cell occurrence and development by regulating cell proliferation, differentiation and inhibition of apoptosis. But the underlying mechanism is unclear.
OBJECTIVE: To investigate the effects of IGF-1 on the proliferation and differentiation of the cultured mouse osteoblasts and the mRNA expression levels of some related factors of Wnt signaling pathway.
METHODS: The medium containing 25 μg/L IGF-1 was added to the mouse osteoblasts cultured in vitro. Cell proliferation was determined using CCK-8 method on days 1, 2, 3, 4, and 5 of culture. Alkaline phosphatase activity was determined with enzyme-linked immunosorbent assay on days 3, 6 and 9 days of culture. On day 3 of culture, total RNA was extracted, and the mRNA expression levels of Wnt-3a, low density lipoprotein receptor-related protein 5 and β-catenin were detected by real time polymerase chain reaction.
RESULTS AND CONCLUSION: 25 μg/L IGF-1 can promote the proliferation of mouse osteoblasts, increase the activity of alkaline phosphatase, and significantly increase the mRNA expression levels of Wnt-3a, low density lipoprotein receptor-related protein 5 and β-catenin (P < 0.05). These findings suggest that IGF-1 can promote the proliferation and differentiation of osteoblasts and Wnt signaling pathway is involved in this process.

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