Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (26): 4777-4780.doi: 10.3969/j.issn.1673-8225.2011.26.007

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Erythromycin and alendronate inhibition of wear particles influences macrophage secretion of interleukin 1, interleukin 2, and tumor necrosis factor alpha

Cui Xue-sheng1,Tian Feng1, Shi Yu-ze1, Zhang Shuai1, Jin Qun-hua2   

  1. 1Graduate School of Ningxia Medical University, Yinchuan  750001, Ningxia Hui Autonomous Region, China
    2Third Department of Orthopedics, Affiliated Hospital of Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China
  • Received:2011-01-09 Revised:2011-03-21 Online:2011-06-25 Published:2011-06-25
  • Contact: Jin Qun-hua, Professor, Master’s supervisor, Third Department of Orthopedics, Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China jinqunhua@sina.com
  • About author:Cui Xue-sheng★, Studying for master’s degree, Associate chief physician, Now working at the Department of Orthopedics, the Second People’s Hospital of Yinchuan City, Graduate School of Ningxia Medical University, Yinchuan 750001, Ningxia Hui Autonomous Region, China hongxiexiashan@yahoo.cn
  • Supported by:

    the Chunhui Plan, No. Z2008-1-75008*

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Abstract:

BACKGROUND: Alendronate has an anti-bone resorption effect which is realized through its inhibitory effect on osteoclasts. Recently, it is confirmed that erythromycin plays a direct role against osteoclasts.
OBJECTIVE: To detect the effects of erythromycin and alendronate inhibited titanium (Ti) particles on interleukin 1(IL-1), IL-6 and tumor necrosis factor alpha (TNF-a) secreted from macrophages.
METHODS: Mouse peritoneal macrophages were separated and cultured, and then divided into erythromycin and alendronate groups. Each group was then divided into six subgroups. Erythromycin group: Group A was treated with macrophages alone, group B with macrophage+Ti particles, group C with macrophage+Ti particles+erythromycin (1μg/L), group D with macrophage+Ti particles+erythromycin (10 μg/L), group E with macrophage+Ti particles+erythromycin (100 μg/L), group F with macrophage+Ti particles+erythromycin (1 000 μg/L). The subgroups and dose of the alendronate group was the same as the erythromycin group. After 24 hours, the levels of IL-1, IL-6 and TNF-a in culture supernatant was detected by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The levels of IL-1, IL-6 and TNF-a were much higher in groups B than those in other groups (P < 0 .05). The levels of IL-1, IL-6 and TNF-a were much lower in groups F than those in groups C (P < 0.05). The same dose of alendronate and erythromycin were not statistically significant (P > 0.05). It was shown that the Ti particles could stimulate macrophages to excrete a great amount of TNF-a ,IL-l and IL-6. Eythromycin and alendronate could significantly inhibit the production of IL-1, IL-6 and TNF-a secreted by Ti particles induced macrophages in a dose dependent manner.

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