Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4461-4464.doi: 10.3969/j.issn.1673-8225.2011.24.022

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High glucose induces podocyte apoptosis and constitutive photomorphogenic 1 expression changes

Wang Xiao-lin, Gan Hua, Du Xiao-gang   

  1. Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2011-01-20 Revised:2011-05-16 Online:2011-06-11 Published:2011-06-11
  • Contact: Gan Hua, Doctor, Professor, Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China cqmusn4@yahoo.com.cn
  • About author:Wang Xiao-lin★, Master, Physician, Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China guowangxia0lin@163.com
  • Supported by:

    the Scientific Research Foundation of Doctors in High Institutes, No. 20070631008*

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Abstract:

BACKGROUND: Studies have shown that constitutive photomorphogenic 1 (COP1) is involved in cell apoptosis.
OBJECTIVE: To observe the effects of high glucose exposure on expression of in vitro cultured podocyte apoptosis and COP1 expression.
METHODS: Mouse podocytes of an immortalized cell line were treated with different concentrations of glucose. Apoptosis index and mortality index at different culture time were evaluated by flow cytometry to screen best dose-effect glucose concentration (30 mmol/L), which was used to treat high-glucose group. Control group and mannitol group (30 mmol/L) were set.
RESULTS AND CONCLUSION: Glucose induced podocyte apoptosis and death in time- and dose-dependent manner in certain concentration (P < 0.05). With increasing glucose concentration, mortality index was significantly elevated, but apoptosis index remained unchanged. Compared with control group, apoptosis index was significantly increased in high glucose group (P < 0.05), but COP1 mRNA and protein expression was significantly decreased (P < 0.05). Results showed that changes in COP1 expression may play a critical role in pathogenesis of high glucose induced podocyte apoptosis.

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