Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4453-4456.doi: 10.3969/j.issn.1673-8225.2011.24.020

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Effect of natural and recombinant hirudin on superoxide dismutase, malonaldehyde and endothelin in rat random skin flap congestion models

Guo Ying-xin, Yin Guo-qian, Li Jia-quan   

  1. Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2010-12-27 Revised:2011-02-13 Online:2011-06-11 Published:2011-06-11
  • Contact: Yin Guo-qian, Chief physician, Doctoral supervisor, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China yingq61@163.com
  • About author:Guo Ying-xin☆, Studying for doctorate, Attending physician, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China nngyx_2008@126. com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang (General program), No. 2010GXNSFA0131410*

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Abstract:

BACKGROUND: Most scholars believed that the effect of hirudin can improve the survival rate of congestion flap based on its antithromotic and antithrombin activity. However, whether it has anti-inflammatory and antioxidant effects remains unclear. The disparity between recombinant hirudin and recombinant hirudin needs to be explored.
OBJECTIVE: To investigate the protective effect of natural hirudin and recombinant hirudin on random skin flap survival in rats vein congestion models. 
METHODS: Thirty Wistar rats were employed to establish animal model of vein congestion. Dorsal random skin flap was prepared on each animal, 10 cm×3 cm in size. The flaps were divided randomly into 3 groups. 5 U natural hirudin, 5 U recombinant hirudin or physiological saline was injected at 1.5 cm and 3.0 cm at distal part of flap immediately, 3 and 5 days after operation. The 7 days flap survival, morphological changes of flap and superoxide dismutase (SOD), endothelin (ET) and malonaldehyde (MDA) levels were measured
RESULTS AND CONCLUSION: The flap survival of the natural and recombinant hirudin groups were greater than that of the control group, with increased SOD level and decreased ET and MDA levels. In particular, the effect was more manifest in the natural hirudin group. The findings demonstrated that hirudin can improve the flap survival rate and SOD content but reduce the rate of flap necrosis, MDA, and ET levels.

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