Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4444-4447.doi: 10.3969/j.issn.1673-8225.2011.24.018

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Expression of platelet-derived growth factor-D in pathogenesis of unilateral ureteral obstruction during renal interstitial fibrosis

Zhang Qian-qian1, Sun Jian-ping1, Gao Yan-xia1, Dong Hui1, Zhou Li-min1, Liu Hong-yan1, Xiao Yu-cui2   

  1. 1Department of Nephrology, 2Department of Rheumatology and Immunology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao  266003, Shandong Province, China
  • Received:2010-12-29 Revised:2011-02-12 Online:2011-06-11 Published:2011-06-11
  • Contact: Sun Jian-ping, Master, Chief physician, Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China sjpqd@163.com
  • About author:Zhang Qian-qian★, Studying for master’s degree, Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China 1985zhangqian@163.com Sun Jian-ping, Master, Chief physician, Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China sjpqd@163.com Zhang Qian-qian and Sun Jian-ping contributed equally to this paper.

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Abstract:

BACKGROUND: The platelet-derived growth factors (PDGF) are highly expressed in the diseased renal, and induce renal interstitial fibrosis through tubulointerstitial cells proliferation, phenotypic modulation and inflammatory cell infiltration.
OBJECTIVE: To observe expression of the PDGF-D in kidney tissues in the process of renal interstitial fibrosis.
METHODS: Totally 60 SD rats were randomly divided into the model and sham-surgery groups. In the model group, rats were constructed unilateral ureteral obstruction models by left ureteral ligation. Animals were sacrificed at 3, 7, 14, 21 and 28 days after operation, and the expression and distribution of the PDGF-D in the kidney tissues were detected by immunohistochemistry, and the expression levels of PDGF-D mRNA in tissue samples were determined by real-time RT-PCR.
RESULTS AND CONCLUSION: There were few PDGF-D expressed in glomerular mesangial cells and vascular smooth muscle cells in the sham-surgery group, but highly expressed in the model group, which were focally observed in interstitial cells in areas of tubulointerstitial fibrosis, and increased with time prolonged. The mRNA level of PDGF-D in the model group was obviously greater than that of the sham-surgery group (P < 0.05). These observations suggest that PDGF-D plays an important role in the pathogenesis of unilateral ureteral obstruction during renal interstitial fibrosis.

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