Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4415-4418.doi: 10.3969/j.issn.1673-8225.2011.24.011

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Role of autologous platelet-rich plasma on fracture healing in rabbits

Zhou Hai-yang, Wang Chen, Geng Zhen   

  1. Department of Orthopedics, the Affiliated Zhongda Hospital of Southeast University, Nanjing  210009, Jiangsu Province, China
  • Received:2011-02-09 Revised:2011-05-12 Online:2011-06-11 Published:2011-06-11
  • Contact: Wang Chen, Professor, Master’s supervisor, Department of Orthopedics, the Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China wangchen@medmail.com.cn
  • About author:Zhou Hai-yang★, Studying for master’s degree, Department of Orthopedics, the Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China haiyangzhou2008@163.com

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Abstract:

BACKGROUND: Platelet-rich plasma (PRP) is often prepared with autalloploid and can be used to promote the fracture healing of flat bone and cancellous bone.
OBJECTIVE: To prepare PRP with Landsberger’s method and to analyzes the therapeutic role of PRP on the fracture healing of radius in rabbits.
METHODS: New Zealand rabbits were randomly divided into a control group and a PRP treated group. Unilateral open femoral fracture models were established in the lower 1/3 radius bone. PRP group received was treated with autologous PRP gel, while control group was only sutured after fracture modeling. Autologous PRP was prepared by Landsberger’s method and then implanted to the fracture site, followed by plaster immobilization of the fracture fragments.
RESULTS AND CONCLUSION: The fracture specimens exhibited varying degrees of callus formation at 1, 2, 4, 6 weeks. X-ray score in the PRP group was significantly higher than that in control group at 4 weeks (P < 0.05). Immunohistochemistry showed that, type I collagen content in the PRP treatment group was significantly higher than that in the control groups for 4 weeks (P < 0.05). PRP accelerates bone fracture healing of rat femurs by increasing expression of type I collagen and the number of bone island.

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