Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (21): 3829-3833.doi: 10.3969/j.issn.1673-8225.2011.21.007

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Tissue engineered cartilage constructed by acellular porcine small intestinal submucosa scaffold combined with autologous chondrocytes

Wu Di1, Xie Yu2, Wang Shao-yun1, Zhu Xiao-song1, Li Qing1, Dong Jian3, Li Shi-he1   

  1. 1Department of Orthopedics, 2Department of Hematology, 3Centre of Biotherapy, First Affiliated Hospital, Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2010-11-12 Revised:2011-03-25 Online:2011-05-21 Published:2011-05-21
  • Contact: Xie Yu, Master, Attending physician, Department of Hematology, First Affiliated Hospital, Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Wu Di☆, Doctor, Associate chief physician, Department of Orthopedics, First Affiliated Hospital, Kunming Medical University, Kunming 650032, Yunnan Province, China xywudi@sohu.com
  • Supported by:

    Social Development Plan of Yunnan Province, No. 2008ZC117M*

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Abstract:

BACKGROUND: It is difficult to achieve a satisfactory repair effect on articular cartilage injury with or without intervention.
OBJECTIVE: To study the feasibility of constructing tissue engineered cartilage using cultivated the autologous chondrocytes combined acellular porcine small intestinal submucosa (SIS) scaffold.
METHODS: The third-generation chondrocytes from porcine knee joint were incubated on porcine SIS and cultured for 48 hours to construct a chondrocyte-scaffold complex. The growth of chondrocytes on the scaffold was observed under light microscope and scanning electron microscope.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that the chondrocytes presented monolayer or multilayer growth on the surface of the SIS scaffold. Immunohistochemistry staining results showed that there was a successive positive expression strap between the chondrocytes and SIS. Under the scanning electron microscope, the cells adhered to the SIS scaffold grew well.

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