Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3633-3635.doi: 10.3969/j.issn.1673-8225.2011.20.006

Previous Articles     Next Articles

Improved stepwise digestion method for chondrocyte primary culture

Tian Feng, Cui Xue-sheng, Zhang Shuai, Shi Yu-ze, Jin Qun-hua   

  1. Third Ward, Department of Orthopedics, Affiliated Hospital of Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China
  • Received:2011-01-21 Revised:2011-04-09 Online:2011-05-14 Published:2011-05-14
  • Contact: Jin Qun-hua, Doctor, Professor, Chief physician, Third Ward, Department of Orthopedics, Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China jinqun-hua@sina. com
  • About author:Tian Feng★, Studying for master’s degree, Third Ward, Department of Orthopedics, Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China tian416@sina.com
  • Supported by:

     University Research Program of Ningxia Educational Committee, No. 2007*; Science and Technology Tackle Key Program of Ningxia Hui Autonomous Region, No. 2008*

PDF

740

Abstract:

BACKGROUND: Combination of trypsin and bacterial collagenase to digest articulate cartilage matrix is widely used at home and abroad. Some studies have shown that the method steps is tedious, process is complicated, and cell is easy to pollution, but the better simple, safe and reliable method has rarely been reported.
OBJECTIVE: To obtain highly purified and large amount of chondrocytes by improved stepwise digestion method.
METHODS: Six 3-week-old New Zealand rabbits were divided into two groups: The experimental group was cultured by two-step enzyme digestion, and the control group was cultured by the traditional enzyme digestion method. The growth condition of chondrocytes was observed in the two group after 1 week, and cell counting and identification was performed.  We estimated improved method to evaluate the influence of cells.
RESULTS AND CONCLUSION: Compared with the traditional method, this method spent shorter time to digest chondrocytes. After 24 hours, the primary chondrocytes in the two groups were mostly round at a suspending state. After 48 hours, the cells began to adhere. The results demonstrated that the improved method shortens digestion time, and easy to obtain a great amount of purified chondrocytes.

CLC Number: