Abstract:

BACKGROUND: The quantitative method using glucose as a tracer has been used for canal leakage assessment. But traditional glucose leakage model has some disadvantages.
OBJECTIVE: To prepare a modified glucose leakage model, to evaluate the sealing ability of three canal sealers of GuttaFlow, AH Plus and iodoform calcium hydroxide.
METHODS: A total of 72 extracted, human single-rooted anterior teeth were used, followed by crown truncation and nickel-titanium root canal preparation. The teeth were randomly divided into three groups (n=20), which were filled root canal with various sealers of GuttaFlow, AH Plus and iodoform calcium hydroxide. Positive and negative control groups were also set (n=6). A modified glucose leakage model was made and used for quantitative evaluation of glucose leakage at 1, 2, 4, 7, 14, 21 and 28 days by glucose oxidase colorimetric method.
RESULTS AND CONCLUSION: There were significant differences of glucose leakage between GuttaFlow group and iodoform calcium hydroxide group, and between AH Plus group and iodoform calcium hydroxide group (P < 0.05). No statistical difference was found in the glucose leakage between GuttaFlow group and AH Plus group (P > 0.05). The modified glucose leakage model is practical, sensitive, economical and accurate. GuttaFlow and AH Plus show better sealing ability than calcium hydroxide based sealer.