Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (34): 6342-6346.doi: 10.3969/j.issn.1673-8225.2010.34.017

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Synergistic role of Huangqi and/or Mailuoning injection with modified collagen in enhancing angiogensis of chicken embryo allantois membrane

Yao Chang1, Gao Wei-wei1, Xue Tao2   

  1. 1 Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing   210029, Jiangsu Province, China; 2 Zhongda Hospital, Southeast University, Nanjing   210009, Jiangsu Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Xue Tao, Chief physician, Professor, Zhongda Hospital, Southeast University, Nanjing 210009 Jiangsu Province, China
  • About author:Yao Chang☆, Doctor, Associate chief physician, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China yaochang67@yahoo.com
  • Supported by:

    Natural Science Foundation of Jiangsu Province, No. BK2007175*; Six Great Talents of Jiangsu Province, No. 06-B-071*

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Abstract:

BACKGROUND: Previous study has found that Huangqi loading modified collagen can promote angiogenesis, and the effect is equal to growth factor-loading effect. It remains unclear that using synergistic Chinese medicine in accordance with traditional Chinese medicine theory is able to further improve the efficacy.
OBJECTIVE: To explore the angiogenic effects of the micro-vessels in the chick chorioallantoic membrane and collagen matrices by loading the Chinese herbal injection of Huangqi and Mailuoning into modified collagen.
METHODS: Five groups were set in the experiment, including blank group without any interfere, control group with only modified collagen implanted into chorioallantoic membrane, Huangqi group with 1 mL of Huangqi injection loading in collagen, Mailuoning group with 1 mL of Mailuoning injection loading in collagen, Huangqi plus Mailuoning group with 0.5 mL Huangqi and 0.5 mL Mailuoning injection loading in collagen. After implanted for 7 days, the samples were explanted from the chorioallantoic membrane. The number of micro-vessels in chorioallantoic membrane was counted, the hemoglobin content and the number of recombinant human vascular endothelial growth factor (rhVEGF165) positive cells in collagen were detected.
RESULTS AND CONCLUSION: For the appearance of macro-samples, the rate of wheel spoke-like vessels and enclosing area of samples by chorioallantoic membrane in each experimental group were better than blank group and control group. The number of micro-vessels in the chorioallantoic membrane, together with hemoglobin content and the number of rhVEGF165 positive cells in the collagen matrices were significantly higher in each experimental group than those in the control group (P < 0.01). Among the experimental groups, the index of Huangqi plus Mailuoning group was significantly higher than the results of Huangqi alone and Mailuoning alone groups (P < 0.05). Loading Huangqi and/or Mailuoning injection in modified collagen can improve angiogenic effects in the chorioallantoic membrane, with the ability of stimulating vessels to grow into the modified collagen. Huangqi plus Mailuoning have a synergetic effect. One of the possible mechanisms is to enhance the expression of rhVEGF165 of vascular endothelial cells.

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