Abstract:

BACKGROUND: The growth factor compounds a small amount of autologous bone marrow and the scaffold materials to construct the inductive tissue engineered bone, thus omitting steps and costs for isolation and in vitro culture of cells, preventing the related pollution and changes of cell trait, it is a feasible method of inducing bone regeneration.
OBJECTIVE: To investigate the feasibility of using nano-nacre artificial bone as a carrier of bone morphogenetic protein and autologous bone marrow, to the curative effect of the constructed inductive tissue engineered bone as a transplant to treat avascular necrosis of femoral head.
METHODS: Fifteen healthy hybrid dogs were used to create avascular necrosis of femoral head models. The animal models were treated by core decompression and nano-nacre artificial bone, respectively. The dogs were sacrificed respectively at 4 and 12 weeks postoperation, for radiography, biomechanics and histology examinations.
RESULTS AND CONCLUSION: The compressive strength in nano-nacre artificial bone group were significantly superior to those in core decompression group and necrosis group (P=0.000). New bone formation in nano-nacre artificial bone group was obviously significant and acted as os endochondrale. In core decompression group, the depressor areas were fibrous connective tissue, new bone formation was hardly found. Results demonstrated that it’s feasible using nano-nacre artificial bone as a carrier of bone morphogenetic protein and autologous bone marrow, the constructed constructed inductive tissue engineered bone can improve compressive strength of femoral head and promote new bone formation and repair necrosis.