Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (33): 6258-6261.doi: 10.3969/j.issn.1673-8225.2010.33.043

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Inhibition effect of mannose-6-phosphate on expression of transforming growth factor beta and its receptor in flexor tendon cells

Shen Yan-qing1, Xia Chang-suo1, Ma Ai-guo2   

  1. 1 Affiliated Hospital of Medical College, Qingdao University, Qingdao   266003, Shandong Province, China; 2 Medical College, Qingdao University, Qingdao   266003, Shandong Province, China 
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Ma Ai-guo, Professor, Doctoral supervisor, Medical College, Qingdao University, Qingdao 266003, Shandong Province, China yzhsyq@163.com
  • About author:Shen Yan-qing★, Studying for master’s degree, Lecturer, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China

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Abstract:

BACKGROUND: Transforming growth factor beta (TGF-β) has an important role in tendon healing and adhesion formation. Inhibiting TGF-β and its receptor expression may prevent adhesions after tendon open.
OBJECTIVE: To study the effects of mannose-6-phosphate, a natural inhibitor of TGF-β, on TGF-β and its receptor production in tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes of rabbit flexor toes.
METHODS: Tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes were isolated from rabbit flexor tendon and cultured separately. All these cells were divided into 2 groups at random, experiment group supplemented with mannose-6-phosphate and control group without mannose-6-phosphate. The expression of TGF-β and TGF-β receptor was quantified with enzyme-linked immunosorbent assay. The expression of TGF-β1 was also assessed with in situ hybridization and immunohistochemistry.
RESULTS AND CONCLUSION: The expression of TGF-β and TGF-β receptor in experiment group was significantly lower than that in control group (P < 0.05). In experimental group, the positive expression of TGF-β1 mRNA and the expression level of intracellular TGF-β1 mRNA in all tendon cells demonstrated significantly lower than those in the control group (P < 0.05). Immunohistochemical staining showed expression of TGF-β1 were significantly lower in all three types of tendon cell cultured with mannose-6-phosphate.

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