Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (33): 6210-6213.doi: 10.3969/j.issn.1673-8225.2010.33.032

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In vitro isolation and cultivation of tissue-engineered skin appendage: Development, detection, regulation and proliferation

Fu Yang, Ma Bing, Xia Zhao-fan   

  1. Institute of Burn Research, Affiliated Changhai Hospital of the Second Military Medical University, Shanghai  200433, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Xia Zhao-fan, Doctor, Chief physician, Doctoral supervisor, Institute of Burn Research, Affiliated Changhai Hospital of the Second Military Medical University, Shanghai 200433, China xiazhaofan@hotmail.com
  • About author:Fu Yang★, Studying for master’s degree, Institute of Burn Research, Affiliated Changhai Hospital of the Second Military Medical University, Shanghai 200433, China future2886@sina.com

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Abstract:

BACKGROUND: All types of tissue-engineered skins have a common challenge, namely, the absence of complete skin function, especially lacking of skin appendages (hair follicles, sweat glands, sebaceous glands, etc.). How to develop the “functional tissue-engineered skin”, that is a complete skin and organ, has become an imminent research direction.
OBJECTIVE: To provide help for solving the problem that it is hard to form skin appendages in artificial composite skins by reviewing progress of isolating or culturing skin appendage-related stem cells and inducing differentiation of skin appendages.                                                                

METHODS: A computer online search using key words of “skin tissue engineering, skin appendages” was performed to search databases of PubMed and CNKI. Inclusive criteria: Articles related to differentiation and proliferation of stem cells into hair follicles, sweat glands, sebaceous glands and other skin appendage using tissue engineering technology. Exclusive criteria: repetitive research or Meta analysis.             

RESULTS AND CONCLUSION: A total of 97 literatures were searched by computer, and 36 papers were further analyzed after excluding 35 unrelated articles and 26 repetitive research. A variety of cells in the human body has the ability to differentiate into skin appendages. Further clarifying the mechanism of embryonic development, in-depth study of stem cells differentiation regulation mechanism, combining growth factors, stem cell biotechnology and bio-materials engineering may develop a real sense of the permanent skin substitute. However, the safety and effect of permanent skin substitute need to be further studied.

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