Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 6001-6005.doi: 10.3969/j.issn.1673-8225.2010.32.024

Previous Articles     Next Articles

Proliferation of vascular endothelial cells and adipose tissue-derived stem cells under in vitro coculture

Wang Fu-ke1, Liu Liu1, He Xiao-guang1, Zhao De-ping2, Yuan Rui-hong1, Dai Xiao-ming1, Li Yi-song1   

  1. 1 Department of Head and Neck Surgery, 2 Experimental Center, First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Liu Liu, Doctor, Professor, Doctoral supervisor, Department of Head and Neck Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China liuliu3939@126.com
  • About author:Wang Fu-ke☆, Doctor, Lecturer, Department of Head and Neck Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China wfk.04@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30960388*; the Associated Specific Foundation Program of Department of Science and Technology of Yunnan Province, No. 2007C0007R*; the Doctor Innovation Foundation Program of Kunming Medical College, No. KM2007D03*

PDF

366

Abstract:

BACKGROUND: Vascular endothelial cells (VECs) have the ability to enhance adipose tissue-derived stem cells (ADSCs) to ossify and new blood vessel can provide nutrition for the ossification of stem cells.
OBJECTIVE: To explore the influence of coculture of VECs and ADSCs on cell proliferation in vitro.
METHODS: We set simple VECs group, 3: 1, 1: 3, 1: 1 coculture group and simple ADSCs group. Cells were incubated in L-DMEM containing fetal bovine serum at 37 ℃ in a 5% CO2 saturated humidity incubator. Morphological changes were observed by inverted microscope. Cell growth curve was drawn using modified MTT assay.
RESULTS AND CONCLUSION: ADSCs and VECs were mixed at 3: 1 and 1: 1 for 14 days. Many synaptic linkages were visible among cells. Partial cells fused into a mass. Cell masses were not observed by other culture methods. With prolonged time, the absorbance of every group was increased gradually and reached a peak in ADSCs, 3: 1, 1: 3 and 1: 1 ratio groups on day 12 and the highest was the 1:1 group, but the time of reaching the peak was on day 10 in the VECs group. The absorbance was gradually reduced under other culture methods. Results have indicated that VECs and ADSCs can stimulate proliferation each other in the system of co-culture in vitro; it can reach the best result when the proportion is 1: 1.

CLC Number: