Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 5988-5991.doi: 10.3969/j.issn.1673-8225.2010.32.021

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Isolation and identification of cancer stem cells in lung cancer cell lines as well as function analysis

Wu Jie, Liu Jing, Wang Xiu-dong, Zhu Ling-yun, Wu Zu-ze , Yu Ai-ping   

  1. Beijing Institute of Radiation Medicine, Academy of Medical Science of Chinese PLA, Beijing  100850, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Wu Zu-ze, Beijing Institute of Radiation Medicine, Academy of Medical Science of Chinese PLA, Beijing 100850, China wuct@nic.bmi.ac.cn
  • About author:Wu Jie☆, Studying for doctorate, Beijing Institute of Radiation Medicine, Academy of Medical Science of Chinese PLA, Beijing 100850, China greenforestwj@sina. com
  • Supported by:

    the National Natural Science Foundation of China,No30770833

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Abstract:

BACKGROUND: It is difficult to maintain the “stem cell” characteristics of cancer stem cells in vitro during proliferation and culture. To obtain cancer stem cells from cancer cell lines that can be kept for a long time is a reliable means.
OBJECTIVE: To investigate whether cancer stem cells exist or not in lung cancer cell strains with similar genetic background and different metastasis potential and function thereof.
METHODS: The adhesion assays on rat collagen or fibronectin were used to assess the metastasis potential of PLA-801D and PLA-801C. The existence of cancer stem cells in the two cell strains was demonstrated with the differentiation potency of their single cell colons and subclones. The biomechanical remodeling potential of the two cell strains was evaluated with collagen gel contraction assays.
RESULTS AND CONCLUSION: Compared with PLA-801C cells, PLA-801D cells showed significantly stronger adhesion on both rat collagen and fibronectin (P < 0.01). PLA-801D cells could form four kinds of single cell clones, and one of them could differentiate into the same four clones in subclones. However, only three kinds of clones were found in PLA801C cells, and none of them could differentiate into the initial three clones in their subclones. PLA-801D cells showed better collagen gel contraction than PLA-801C cells (P < 0.05). These results indicate that a grouplet of cells in PLA-801D cells show self-renewal and multi-directional differentiation characteristic of cancer stem cells. Moreover, PLA-801D cells display good biomechanical remodeling potential, indicating its good remodeling capacity for extracellular matrix.

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