Abstract:

BACKGROUND: During the dentofacial development, the first branchial arch as a transitional structure plays an important role. At present, domestic and international research data for the first branchial arch mesenchymal cells were relatively few.
OBJECTIVE: To isolate and culture the mesenchyme cells from the first branchial arch of the mice embryos.
METHODS: The first branchial arch primordia from E9.5 embryos were dissected under a microscope. Explanted culture and improved enzyme digestion were used to primarily culture. The growth curve, population doubling time, cells proliferation and morphology were studied and specific markers were detected to study biological characteristics of cells.
RESULTS AND CONCLUSION: The cells were grown to confluency for 7-10 days, with abundant epithelial cells. Improved enzyme digestion of primary culture had less epithelial-like cells, the cells were grown to confluency for 2-3 days. The growth curve, population doubling time and morphology were similar in both groups after passage. Anti-CD57/HNK-1 and anti-vimentin were all positive. Results have indicated that sufficient and high purity ectomesenchymal cells could be harvested in short time by improved enzyme digestion.