Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (24): 4449-4453.doi: 10.3969/j.issn.1673-8225.2010.24.018

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Changes in contractile function of cardiocytes following overexpressing beta 2 adrenergic receptor in canines and the underlying mechanisms

Shan Yu1, Gong Hai-bin 1，2,3, Wang Lei2, Wang Xiu-li2, Lü Qian2, Zhang Qing-lin2, Liu Ying2, Wang Jie2, Pang Hui2, Su Jing-li1, Wang Zhen-quan2   

  1. 1 edical College of Southeast University, Nanjing  210009, Jiangsu Province, China; 2 Xuzhou Cardiovascular Disease Institute, Xuzhou  221009, Jiangsu Province, China; 3 Department of Cardiology, Xuzhou Central Hospital, Xuzhou  221009, Jiangsu Province, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Gong Hai-bin, Doctor, Professor, Master’s supervisor, Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China; Xuzhou Cardiovascular Disease Institute, Xuzhou 221009, Jiangsu Province, China; Department of Cardiology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China ghbxzh@yahoo.com.cn
  • About author:Shan Yu★, Studying for master’s degree, Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China shanyuat@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30572073*; the Natural Science Foundation of Jiangsu Province, No. BK2005428*; the Outstanding Medical Academic Leader Program of Jiangsu Province (135 Program), No. RC2007024*?; the Major Research Projects of Health Department of Jiangsu Province, No. K200402*?; the Social Development Project of Xuzhou, No. XM09B073*

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Abstract:

BACKGROUND: Previous studies have found that cardiocytes overexpressing β-adrenergic receptors (β-AR) can improve its contractile function in transgenic mice, rats and other small animals, but experimental reports about the large mammals are few.
OBJECTIVE: To observe changes and mechanisms of contractile function following cardiocytes overexpressing β2-AR in canines.
METHODS: Canine cardiocytes were isolated by collagenase II and transfected by adenovirus β2AR. The expression of β2AR protein of cardiocytes, the level of intracellular cyclic adenosine monophosphate (cAMP) at basic state and maximum systolic states, and the changes of contractile function were observed at 48 hours after operation. 
RESULTS AND CONCLUSION: Compared with the control group, the expression of β2AR protein of cardiocytes and the level of the intracellular cAMP in the adenovirus β2AR group were increased (P < 0.05). The basal contraction amplitude of cadiocytes in adenovirus β2-AR group was also increased significantly (P < 0.05), but the max contraction amplitude of cardiocytes was not changed significantly between two groups. The study suggested that the over-expression of β2AR could increase the level of intracellular cAMP and improved the contractile function of cadiocytes in canines.

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