Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (24): 4407-4410.doi: 10.3969/j.issn.1673-8225.2010.24.009

Previous Articles     Next Articles

Segmental artery ligation for establishment of local spinal cord ischemic injury model in rabbits

Tan Mei-yun1, Guo Xing2, Wang Yuan-hui1   

  1. 1 Department of Bone and Joint Surgery, 2 Department of Burns and Plastic Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Guo Xing, Master, Attending physician, Department of Burns and Plastic Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuang Province, China gx412@126.com
  • About author:Tan Mei-yun☆, Doctor, Attending physician, Department of Bone and Joint Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China drtmy@126.com

PDF

516

Abstract:

BACKGROUND: Previous studies showed that a stable model of spinal cord injury can be established using a method of lumbar arteries ligation in retroperitoneum. However, the precise ligation area is unclear. 
OBJECTIVE: To establish a rabbit model of local spinal cord ischemic injury produced by segmental artery ligation.
METHODS: A total of 16 adult rabbits were randomly divided into the control and model groups, with 8 animals in each group. Rabbits in the model group experienced ligation of segment artery in retroperitoneum. There was no treatment in the control group. The nerve function of spinal cord was evaluated by modified Tarlov score and the pathological changes of lumbar segmemal spinal cord were observed with the hematoxylin-eosin staining at 3 days after operation.
RESULTS AND CONCLUSIONS: Paralysis was evidenced in the model group and the Tarlov scores were smaller than that of the control group (P < 0.05). Acute inflammatory lesion and neuronal necrosis were observed in the model group. Results showed that a stable model of local spinal cord ischemic injury can be established via ligating L1-3 lumbar arteries approach retroperitoneum.

CLC Number: