Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (15): 2740-2743.doi: 10.3969/j.issn.1673-8225.2010.15.019

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Effects of melanocortin-3 receptor gene A167T on the body weight trait of Beagle dogs 

Du Peng, Ba Cai-feng, Zhou Yan-bin, Zhang Yi-bo, Song Hui-juan   

  1. Center of Experimental Animal, Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Ba Cai-feng, Master, Professor, Master’s supervisor, Center of Experimental Animal, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China bacaifeng@163.com
  • About author:Du Peng★, Studying for master’s degree, Center of Experimental Animal, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China yxydp@126.com
  • Supported by:

    the Foundation of Science and Technology Department of Liaoning Province, No. 2007408001-6*

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Abstract:

BACKGROUND: Melanocortin-3 receptor (MC3R) gene mutation is an important factor in weight gain.
OBJECTIVE: To analyze the relationship between MC3R gene mutations and body weight traits in Beagle dogs.
METHODS: The blood samples were taken from 112 Beagle dogs, and their body weights were recorded, then their genomic DNA was extracted. The A167T site of MC3R gene was detected by the methods of AS-PCR, and mutant-type MC3R gene was sequenced. The relationships between MC3R genotypes distribution and body weight were observed.
RESULTS AND CONCLUSION: The A167T site of Beagle canine MC3R gene was found with two alleles A and T and three genotypes AA, AT and TT. Statistical analysis showed that the base substitution of T167A had significant influence on canine body weight (P < 0.05). It suggested that MC3R gene mutation can lead to a significant increase in canine body weight, which can be used as a candidate gene for body weight labeling.

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