Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (15): 2735-2739.doi: 10.3969/j.issn.1673-8225.2010.15.018　

Previous Articles     Next Articles

Determination of gastrodin level in serum containing formula for calming the liver and suppressing yang and investigation of drug serum effects on vascular smooth muscle cell avtivity in rats

Li Xiang-ping1, Luo Yan-hong2, Zhong Guang-wei3, Yin Tao1, Xiang Ling-li3, Li Wei3, Chen Guo-lin1   

  1. 1 Department of Pharmacy, 2 Department of Emergency, 3 Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha  410008, Hunan Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Zhong Guang-wei, Master, Attending physician, Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China zgw7512@sina.com
  • About author:Li Xiang-ping★, Master, Pharmacist- in-charge, Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China xylxping@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30500644*; the Science and Technology Projects of Traditional Chinese Medicine of Hunan Province, No. 2009047*

Abstract:

BACKGROUND: The formula for calming the liver and suppressing yang is a Chinese herbal medicine compound preparation composed complex chemical constituents, thus, it is difficult to determine its serum levels. However, as a principal drug for this formula, the level of gastrodin is easy to measure.   
OBJECTIVE: With a method of serum-pharmacology, to explore the prepare method of serum containing formula for calming the liver and suppressing yang, and to observe its effects on the activity of vascular smooth muscle cells (VSMCs) in rats. 
METHODS: Rats were given 1g formula for calming the liver and suppressing yang extract at 8: 00 and 16:00 continuous for 3 days or 7 days, respectively. Then additional 2 g drugs were administrated at 8:00 in the next days of 3 or 7 days. The blood was taken from heart at prior to and 0.5, 1, 2, 3 and 5 hours after the last administration. Gastrodin levels in these serums were determined by high performance liquid chromatogram. Subsequently, inactivated serum containing formulae for calming the liver and suppressing yang with different concentrations was used to culture VSMCs, and the inhibitory rates of drug serum on VSMCs activity were observed at 24 hours after culture using MTT assay. 
RESULTS AND CONCLUSION: Concentration of gastrodin reached the highest level at 1 hour after last administration of formulae for calming the liver and suppressing yang in the 3-day administration or 7-day administration groups. However, the gastrodin level was rapidly decreased in the 3-day administration group, which maintained for a long time in the 7-day administration group. A linearity was obtained over the concentration of 0.045-5.6 mg/L, r=0.999 2. The average recovery was 93.88% with relative standard deviation (RSD)within day=1.86%. Serum containing each herbal medicine could inhibit VSMCs activity at different concentrations. The greatest growth inhibitory ratio of VSMCs was obtained using 5.0% concentration serum containing Chinese herbal medicine (P < 0.01), and compared with the 3-day administration group, the VSMCs of the 7-day group exhibited remarkable growth inhibition ratio (P < 0.01). Accordingly, the optimal time for blood collecting is at 1 hour after a 7-day successive administration. The serum containing formula for calming the liver and suppressing yang can inhibit the growth of VSMCs.

CLC Number: