Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (15): 2718-2721.doi: 10.3969/j.issn.1673-8225.2010.15.014

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Eftects and mechanisms of spironolactone on corneal neovascularization after corneal alkali burn in rats   

Guo Zuo-feng1, Pang Dong-bo1, Shan Wei2   

  1. 1 Department of Ophthalmology, First Affiliated Hospital of Liaoning Medical University, Jinzhou   121001, Liaoning Province, China; 2 Department of Anatomy, Liaoning Medical University, Jinzhou   121001, Liaoning Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Pang Dong-bo, Doctor, Department of Ophthalmology, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China pang2000@163.com
  • About author:Guo Zuo-feng★, Studying for master’s degree, Physician, Department of Ophthalmology, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China 402345068@qq.com

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Abstract:

BACKGROUND: Spironolactone is an aldosterone antagonist, which was confirmed to possess inhibition effect on the angiogenesis in vitro and in vivo.
OBJECTIVE: To verify the effect of spironolactone on corneal neovascularization (CRNV) of the rats induced by alkali burns.
METHODS: Corneal models induced by alkali burn were established on 36 SD rats, which were random divided into the experimental group (treated orally with SL 100mg/kg once a day) and control group (treated with the same volume of normal saline). Additional 6 rats were served as normal control group without treatment. The length and area of CRNV in each group were examined by microscope at days 4, 7 and 14 after operation. Six rats of each group were killed and the expressions of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in cornea were detected with immunohistochemistry and computer image analysis system.
RESULTS AND CONCLUSION: Corneal neovascularization and inflammatory cells were not be found in the normal corneal, and there was a little expression of VEGF in the normal corneal epithelium and stroma but not detected the expression of MMP-2. Compared with control group, the area of blood vessels was significant smaller and the expression of VEGF and MMP-2 were significant lower (P < 0.05) at different observing times. Spironolactone can inhibit the occurrence of CRNV by down regulating the expression of VEGF and MMP-2.

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