Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (15): 2684-2687.doi: 10.3969/j.issn.1673-8225.2010.15.006

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Bone turnover in complete spinal cord transaction rats: Influence of ultra-early hyperbaric oxygen treatment 

Tong Min1, Wu Xian-ping2, Chen Jun1, Liu Min3   

  1. 1 Animal Laboratory, 2 Institute of Endocrine and Metabolic Disease, 3 Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha   410011, Hunan Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Liu Min, Master, Associate professor, Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China liumin22626@126.com
  • About author:Tong Min★, Master, Technician-in-charge, Animal Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China tongmin22626@163.com
  • Supported by:

    the Program of Department of Science and Technology of Hunan Province, No. 2008SK3108*

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Abstract:

BACKGROUND: Hyperbaric oxygen (HBO) treatment can promote the recovery of incomplete spinal cord injury, and the earlier, the better was.
OBJECTIVE: To explore the influence of ultra-early HBO treatment on bone turnover in complete spinal cord transaction rats.
METHODS: Sprague Dawley rats were randomly divided into sham operation, model and HBO groups. Rats in sham operation group underwent laminectomy, while rats in the other 2 groups underwent complete spinal cord transection at the level of the tenth thoracic vertebra. Rats in HBO group were put in animal oxygen chamber at 3 hours after operation, 222.92 kPa atmosphaera absolutus, oxygen concentration 97%-99%, 10 days as 1 course of treatment, for 3 courses, twice a day in first course, once a day in second and third courses, respectively, with a 6 days’ interval between courses. Model group did not receive HBO treatment. At 6 weeks after model estahblishment, the level of the serum bone alkaline phosphatase (BAP), serum osteocalcin (sOC), serum cross-linked N-telopeptide of collagen type Ⅰ(sNTX) and urinary deoxypyridinoline (DPD) were measured by enzyme linked immunosorbent assay.
RESULTS AND CONCLUSION: The levels of BAP and sOC of HBO group was much higher than that of the model group (P < 0.05). The level of BAP and sOC of the model group was much higher than that of the sham operation group (P < 0.05). The level of sNTX and urine DPD of HBO group was much lower than that of the model group (P < 0.05). The level of sNTX and urine DPD of the model group was much higher than that of the sham operation group (P < 0.05). These suggested that ultra-early HBO treatment affects bone turnover in rats with complete spinal cord injury, contributes to bone formation, and inhibits bone resorption.

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