Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (15): 2675-2679.doi: 10.3969/j.issn.1673-8225.2010.15.004

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Changes in G1 phase regulatory protein of osteoblasts in ovariectomized osteoporosis rats 

Wu Yin-sheng1, Lin Yan-ping1, Lu Tian-xiang2, Lin Yu1, Huang Yun-mei3, Huang Mei-ya3   

  1. 1 Institute of Bone Disease, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China; 2 Department of Orthopaedics, Quanzhou First Hospital, Quanzhou  362000, Fujian Province, China; 3 Key Laboratory of Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Lin Yan-ping, Institute of Bone Disease, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, Fujian Province, China Lyp66@126.com
  • About author:Wu Yin-sheng☆, Doctor, Assistant researcher, Institute of Bone Disease, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, Fujian Province, China Wings197810@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30572402*; Key Program of Social Development Department of Fujian Provincial Department of Science and Technology, No. 2009Y0028*; Youth Talent Program of Science and Technology Department of Fujian, No. 2008F3054*; Open Subjects of Fujian Provincial Key Laboratory of Integrative Medicine for Senile Diseases, (Fujian University of Traditional Chinese Medicine) · Chen Ke-ji Foundation for the Development of Integrative Medicine, No. 2008J1004-30, CKJ2008067*

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Abstract:

BACKGROUND: G1 phase regulatory protein plays an important role in regulating cell reproductive cycle. However, there are few reports concerning research of G1 phase regulatory protein of osteoblasts, and the relationship between postmenopausal osteoporosis and the G1 phase regulatory protein of osteoblasts have not been explained.
OBJECTIVE: To observe the change of G1 phase regulatory protein of osteoblasts in the ovariectomized osteoporosis rats, and to investigate the pathogenesis of postmenopausal osteoporosis.
METHODS: Totally 100 6-month-old SD female rats were randomly divided into sham surgery and model groups, with 50 animals in each group. Rats in the model group were performed bilaterally ovariectomy, those in the sham surgery group were subjected to sham surgery. Ten rats of each group were sacrificed at 4,8,12,18 and 24 weeks after operation, respectively. The expression of Cyclin D1, CDK4, and p21 protein in osteoblasts of lumbar vertebra were measured by immunohistochemistry.
RESULTS AND CONCLUSION: Cyclin D1 and CDK4-positive expression mainly located at osteoblasts which in the superficies of bone trabecula. There was Cyclin D1 and CDK4 positive expression in the sham surgery group, but was obviously smaller than that of the model group. The location of p21 positive expression was similar to Cyclin D1. Obviously positive expression of p21 appeared in the bone tissue of both groups, in which that of the model group maintained at a high level, and higher than that of the sham surgery group at different periods after operation (P < 0.01). The results demonstrated that Cyclin D1, CDK4 and p21 in osteoblasts were hyper-expressed in the process of osteoporosis in ovariectomized rats. It suggested that faster osteoblast proliferation and inhabited osteoblast cell cycles result in relative insufficiency of osteoblast, thus the bone formation is smaller than bone resorption, eventually, lead to the occurrence of osteoporosis.

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