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    28 January 2025, Volume 29 Issue 3 Previous Issue    Next Issue
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    Stress and morphological characteristics of intervertebral foramen of cervical rotation-traction manipulation for treating cervical spondylotic radiculopathy: a three-dimensional finite element analysis
    Wang X, Wang Hm, Chen Sh, Feng Tx, Bu Hm, Zhu Lg, Chen Dd, Wei X
    2025, 29 (3):  441-447.  doi: 10.12307/2025.104
    Abstract ( 142 )   PDF (1664KB) ( 167 )   Save
    BACKGROUND: Cervical rotation-traction manipulation is effective and safe in the treatment of cervical spondylotic radiculopathy, and has been widely used in clinical work. However, its effects on the biomechanics of cervical vertebra and intervertebral disc and the area of intervertebral foramen have not been systematically clarified.
    OBJECTIVE: Based on the finite element analysis technique, a relevant research and analysis were carried out to provide digital evidence for the mechanism of effect of cervical rotation-traction manipulation in the treatment of cervical spondylotic radiculopathy.
    METHODS: The CT image data of a volunteer with no neck diseases were selected as the finite element model material at its left-handed physiological limit position. The initial construction of the finite element model was completed by Mimics 19.0 software, Geomagic Studio 2013 software, Hypermash 14.0 software, and ANSYS Workbench 2020 R2 software, respectively. Based on the literature, the grid division of cervical structure and the assignment of elastic modulus and elastic coefficient were completed. Based on the previous work of the team, the mechanical effects of cervical rotation-traction manipulation were simulated on the model. Effects of cervical rotation-traction manipulation on the mechanical parameters of each vertebral body and intervertebral disc in C3-T1 segment and on the cervical lateral foramen area were analyzed.
    RESULTS AND CONCLUSION: (1) During cervical rotation-traction manipulation, the stress of bone structure was significantly higher than that of soft tissue such as intervertebral disc. (2) When operating the technique, the stress at the top of each cervical vertebra was higher, the stress at the bottom was lower, and the stress at the facet joint and transverse process was lower. The stress at the top of the intervertebral disc was lower, the stress at the bottom was higher, but the highest point of the intervertebral disc stress was outside the top. (3) In addition, after loading the lifting force, the projected area of the C6/C7 
    intervertebral foramen increased significantly compared with that before loading. (4) It is indicated that the cervical rotation-traction manipulation has the mechanical characteristics of changing the stress structure of the cervical spine itself, and can expand the C6/7 intervertebral cervical foramen area on the opposite side of the patient’s cervical rotation, so as to achieve the purpose of treating cervical spondylotic radiculopathy.
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    Establishment and stress analysis of a finite element model for adolescent cervical disc herniation
    Zhao Yuxin, Liang Liang, Jin Feng, Xu Yangyang, Kang Zhijie, Fang Yuan, He Yujie, Wang Xing, Wang Haiyan, Li Xiaohe
    2025, 29 (3):  448-454.  doi: 10.12307/2025.113
    Abstract ( 157 )   PDF (1718KB) ( 146 )   Save
    BACKGROUND: Cervical disc herniation can cause pain in the neck and shoulder area, as well as radiating pain in the upper limbs. The incidence rate is increasing year by year and tends to affect younger individuals. Fully understanding the biomechanical characteristics of the cervical spine in adolescents is of great significance for preventing and delaying the onset of cervical disc herniation in this age group.
    OBJECTIVE: To reconstruct cervical spine models for both healthy adolescents and adolescent patients with cervical disc herniation utilizing finite element analysis techniques, to analyze the motion range of the C1-T1 cervical vertebrae as well as the biomechanical characteristics of the annulus fibrosus, nucleus pulposus, endplates, and the cartilage of the small joints.
    METHODS: A normal adolescent’s cervical spine and an adolescent patient with cervical disc herniation were selected in this study. The continuous scan cervical spine CT raw image data were imported into Mimics 21.0 in DICOM format. The C1-T1 vertebrae were reconstructed separately. Subsequently, the established models were imported into the 3-Matic software for disc reconstruction. The perfected models were then imported into Hypermesh software for meshing of the vertebrae, nucleus pulposus, annulus fibrosus, and ligaments, creating valid geometric models. After assigning material properties, the final models were imported into ABAQUS software to observe the joint motion range of the C1-C7 cervical vertebrae segments under different conditions, and to analyze the biomechanical characteristics of the annulus fibrosus, nucleus pulposus, endplates, and small joint cartilage of each cervical spine segment.
    RESULTS AND CONCLUSION: (1) In six different conditions, the joint motion range of the C1 vertebra in the cervical spine models of both normal adolescent and adolescent patient with cervical disc herniation was higher than that of the other vertebrae. Additionally, the joint motion range of each cervical spine segment in normal adolescent was greater than that in adolescent patient with cervical disc herniation. (2) In the cervical spine model of normal adolescent, the maximum stress values in the annulus fibrosus and nucleus pulposus were found on the left side during C2-3 flexion conditions (0.43 MPa and 0.17 MPa, respectively). In the cervical spine model of adolescent patient with cervical disc herniation, the maximum stress values were found on the left side during C7-T1 flexion conditions (0.54 MPa and 0.18 MPa, respectively). (3) In the cervical spine model of normal adolescent, the maximum stress value on the endplate was found on the left side of the upper endplate of C3 during flexion conditions (1.46 MPa). In the model of adolescent patient with cervical disc herniation, the maximum stress value on the endplate was found on the left side of the lower endplate of C7 during flexion conditions (1.32 MPa). (4) In the cervical spine model of normal adolescent, the maximum stress value in the small joint cartilage was found in the C2-3 left rotation conditions (0.98 MPa). In adolescent patient with cervical disc herniation, the stress in the small joint cartilage significantly increased under different conditions, especially in C1-2, with the maximum stress found during left flexion (3.50 MPa). (5) It is concluded that compared to normal adolescent, adolescent patient with cervical disc herniation exhibits altered cervical curvature and a decrease in overall joint motion range in the cervical spine. In adolescent with cervical disc herniation, there is a significant increase in stress on the annulus fibrosus, nucleus pulposus, and endplates in the C7-T1 segment. The stress on the left articular cartilage of the C1-2 is notable. Abnormal cervical curvature may be the primary factor causing these stress changes.
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    Finite element analysis of three internal fixation modalities for treatment of Pauwels type III femoral neck fractures under different loading conditions
    Li Zhenggang, Shang Xuehong, Wu Zhang, Li Hong, Sun Chaojun, Chen Huadong, Sun Zhe, Yang Yi
    2025, 29 (3):  455-463.  doi: 10.12307/2025.133
    Abstract ( 89 )   PDF (5202KB) ( 132 )   Save
    BACKGROUND: There is still no consensus on the optimal internal fixation for the treatment of Pauwels III femoral neck fracture, and most of the related finite element analyses have been performed using a single simplified loading condition, and the biomechanical properties of commonly used internal fixation devices need to be further investigated.
    OBJECTIVE: To analyze the biomechanical characteristics of Pauwels III femoral neck fractures treated with cannulated compression screw, dynamic hip screw, and femoral neck system by finite element method under different loading conditions of single-leg standing loads and sideways fall loads.
    METHODS: The DICOM data of healthy adult femur were obtained by CT scanning, imported into Mimics 15.0 software to obtain the rough model of bone tissue. The data exported from Mimics were optimized by Geomagics software, and then three internal fixation models were built and assembled with the femur model according to the parameters of the clinical application of the cannulated compression screw, dynamic hip screw, and femoral neck system by using Pro/E software. Finally, the three internal fixation models were imported into Ansys software for loading and calculation to analyze the stress distribution and displacement of the femur and the internal fixation under different working conditions of single-leg standing loads and sideways fall loads, as well as the stress characteristics of the calcar femorale and Ward’s triangle.
    RESULTS AND CONCLUSION: (1) Under the single-leg standing load and the sideways fall load, the proximal femoral stress of the three internal fixation models was mainly distributed above the fracture end of the femoral neck. The peak stress of the proximal femoral end, fracture end, Ward triangle, and calcar femorale of the three internal fixation models were the smallest in the femoral neck system model and the largest in the cannulated compression screw model. (2) Under the single-leg standing load and the sideways fall load, the peak displacement of the proximal femur of the three internal fixation models was all located at the top of the femoral head, and the peak displacement was the smallest in the femoral neck system model and the largest in the cannulated compression screw model. (3) The peak displacement of the three internal fixation models was all located at the top of the internal fixation device under the single-leg standing and sideways fall loading conditions, and the peak displacement values were the smallest in the femoral neck system internal fixation model and the largest in the cannulated compression screw internal fixation model. (4) The internal fixation stress of the three internal fixation models was mainly distributed in the area near the fracture end of the internal fixation device under the single-leg standing and sideways fall loads, and the peak value of internal fixation stress was the smallest in the femoral neck system model and the largest in the cannulated compression screw model. (5) These results suggest that the mechanical stability of the femoral neck system is the best, but there may be a risk of stress shielding of the fracture end and calcar femorale. The stress of the internal fixation device of the femoral neck system is more dispersed, and the risk of internal fixation break is lower.
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    Finite element analysis of optimization of femoral prosthesis implantation position in unicompartmental knee arthroplasty in osteoporotic patients
    Liu Mengfei, Chen Gang, Shi Yihan, Zeng Lin, Jiang Kan, Yilihamujiang•Wusiman
    2025, 29 (3):  464-470.  doi: 10.12307/2025.107
    Abstract ( 123 )   PDF (1291KB) ( 124 )   Save
    BACKGROUND: The reasonable implantation range of femoral prosthesis in unicompartmental knee arthroplasty in patients with osteoporosis has not been investigated, and previous studies have often been based on unicompartmental knee arthroplasty models in normal bone, with fewer mechanical studies in models with non-normal bone. Complications after unicompartmental knee arthroplasty have been shown to be highly associated with osteoporosis. 
    OBJECTIVE: To analyze the biomechanical effects of the coronal inclination of the Sled fixed platform femoral prosthesis on unicompartmental knee arthroplasty in patients with osteoporosis and to find the correlation between osteoporosis and mid- and long-term complications after unicompartmental knee arthroplasty.
    METHODS: Based on the digital imaging technology to obtain the data of the knee joint and prosthesis, a normal bone knee model is then created by using specialized software such as Mimics and Geomagic studio. Based on a validated normal bone knee model, an osteoporotic knee model was created by changing the material parameters. Totally 14 unicompartmental knee arthroplasty finite element models were created using Sled fixed platform femoral prosthesis: standard position (0°), varus and valgus angles: 3°, 6°, 9° in the normal bone and osteoporosis groups. Stress changes on the surface of polyethylene liner, cancellous bone under tibial prosthesis, and cortical bone were calculated and analyzed in all unicompartmental knee arthroplasty models.
    RESULTS AND CONCLUSION: (1) In the osteoporotic models, the high stress values of the polyethylene liner surface and the cancellous bone under the tibial prosthesis increased with the increase of the tilt angle of the femoral prosthesis, and the high stress values of the cortical bone surface under the tibial prosthesis increased with the increase of the prosthesis valgus angles and decreased with the increase of the varus angles. (2) For the polyethylene liner surface as well as the subcortical bone surface of the tibial prosthesis, the high stress values of the models for each inclination angle in the osteoporosis group were greater than those of the corresponding models in the normal bone group. For the surface of the cancellous bone under the tibial prosthesis, the high stress values of the tilt angle models of the osteoporosis groups were smaller than those of the normal bone groups. (3) Osteoporosis may cause biomechanical abnormalities in the internal structures of the knee after unicondylar replacement, increasing the potential risk of postoperative aseptic loosening of the prosthesis and periprosthetic fractures. Varus and valgus of the femoral prosthesis in the coronal plane should be avoided as much as possible when performing medial unicompartmental knee arthroplasty with a Sled fixation platform in osteoporotic knees.
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    Biomechanical characteristics of walking in patients with idiopathic scoliosis: cross-sectional analysis of three-dimensional motion capture
    Gou Yanyun, Hou Meijin, Jiang Zheng, Chen Shaoqing, Chen Xiang, Gao Yuzhan, Wang Xiangbin
    2025, 29 (3):  471-477.  doi: 10.12307/2025.115
    Abstract ( 195 )   PDF (1083KB) ( 290 )   Save
    BACKGROUND: The biomechanical analysis of scoliosis cases is limited, with only independent analysis focusing on the spine or lower limbs, thus lacking a comprehensive evaluation of the multidimensional body. As a result, it becomes challenging to reflect the movement relationship between the trunk and lower limbs during daily activities, which hinders comprehensive clinical treatment guidance.

    OBJECTIVE: To explore the relationship between different segments of the spine and the kinematics/kinetics of the lower limbs during gait activities by measuring spinal kinematics in scoliosis patients, to provide a comprehensive and multi-level assessment of the biomechanical differences between scoliosis patients and the normal population, consequently offering evidence-based guidance for the prevention and treatment of scoliosis.
    METHODS: A cross-sectional study was conducted from July 2020 to June 2021 at the Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine in Fuzhou University City. A total of 28 scoliosis patients and 28 normal individuals in the same age group were included. Three-dimensional motion capture system was used to capture gait kinematic data at a sampling frequency of 100 Hz. Two force plates (AMTI 400600, sampling frequency 1 500 Hz) were embedded in a 10-meter-long 2.4-meter-wide level ground walkway (with an effective data collection length of 4 m) to collect kinetic data. The differences in spatial-temporal parameters, kinematics, and kinetics of gait between the two groups were compared. Immediately after inclusion, all subjects underwent full spinal X-ray measurements to compare the differences between the scoliosis and normal groups.
    RESULTS AND CONCLUSION: (1) Patients with scoliosis exhibited reduced relative rotational range of motion between the shoulder and trunk, as well as between the thorax and pelvis, compared to the normal group (P < 0.05). However, the rotational range of motion in the pelvis was larger in patients with scoliosis compared to the normal group (P < 0.05). (2) Patients with scoliosis showed decreased range of motion in the hip and knee joints, as well as reduced peak torque in hip joint flexion and extension, and lower peak values of ground reaction forces in the concave and convex directions, in comparison to the normal group (P < 0.05). (3) Patients with scoliosis demonstrated greater asymmetry indices in knee joint range of motion, relative rotational range of motion between the shoulder and trunk, and between the thorax and pelvis, when compared to the normal group (P < 0.05). (4) These findings illustrate a rigid movement pattern among the shoulder, thorax, and pelvis in patients with scoliosis during level walking. There is a reduction in range of motion in the hip and knee joints, as well as decreased peak torque values in hip joint flexion and extension, and ground reaction forces in the concave and convex directions. These characteristics can serve as foundational elements for assessing rehabilitation and developing treatment plans.

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    Biomechanical characteristics of lower extremities during counter movement jump in male patients with functional ankle instability
    Wang Zilong, Meng Xin, Zhang Zhiqi, Xie Yu, Meng Lingyue, Zhang Qiuxia, Kong Lingyu
    2025, 29 (3):  478-485.  doi: 10.12307/2025.135
    Abstract ( 161 )   PDF (1341KB) ( 367 )   Save
    BACKGROUND: As the end bearing joint of the human body, the ankle joint bears the top-down pressure of the body, which leads to the ankle joint is easy to be damaged in the movement, can induce functional ankle instability, which negatively affects daily life. The study of lower extremity biomechanics in patients with functional ankle instability during counter movement jump is of great significance for scientific training, prevention of ankle injury, and clinical rehabilitation after injury.
    OBJECTIVE: To investigate the kinetics and kinematics of lower limbs in the longitudinal jumping of functional ankle instability population. 
    METHODS: From March to September 2023, 15 male patients with functional ankle instability and 15 healthy people, aged 22-28 years old, were recruited in Soochow University. All subjects completed counter movement jump experiment. Vicon infrared high-speed motion capture system and Kistler three-dimensional force measuring table were used to simultaneously collect the lower limb kinematics and kinetics indexes of the two groups of subjects at the take-off stage of counter movement jump, the instant off the ground, the initial landing moment and the peak moment of vertical ground reaction force.
    RESULTS AND CONCLUSION: (1) At the instant off the ground, the affected side of the functional ankle instability group showed smaller knee internal rotation moment (P=0.020) and smaller ankle internal rotation moment (P=0.009) compared with the affected side of the healthy control group. (2) At the moment of landing, the affected side of the functional ankle instability group showed a smaller hip flexion angle than the affected side of the healthy control group (P=0.039). Compared with the healthy control group, functional ankle instability group showed smaller hip abduction angle (P=0.022), smaller knee varus angle (P=0.010), larger knee external rotation angle (P=0.021), smaller ankle varus angle (P=0.004), and smaller external ankle rotation angle (P=0.008). (3) At the peak of vertical ground reaction force, functional ankle instability group showed a smaller ankle varus angle than healthy control group (P=0.044). (4) The results showed that the lower limb biomechanical characteristics of the patients with functional ankle instability were abnormal compared with the healthy people during counter movement jump, which mainly showed the changes of the kinematics and kinetics indexes of the lower limb joints in the sagittal plane and the frontal plane at the moment of lift-off and landing. These changes reflect that people with functional ankle instability adopt rigid take-off and landing patterns when performing counter movement jump, tend to transfer the load of the affected ankle joint to other joints of the lower limb, and show compensatory phenomenon of the healthy lower limb. Therefore, detection and correction of abnormal biomechanical features should be a part of rehabilitation training for those with functional ankle instability.
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    Quantification of in vivo biomechanics and analysis of influencing factors in cervical spine fixed-point rotation manipulation
    Liang Jiyao, Zhou Honghai, Wei Guikang, Su Shaoting, Chen Longhao, He Xinyu, Liu Liangpu
    2025, 29 (3):  486-492.  doi: 10.12307/2025.111
    Abstract ( 124 )   PDF (1684KB) ( 113 )   Save
    BACKGROUND: Fixed-point rotation manipulation of cervical spine is a mechanical operation with high technical requirements, but the biomechanics of fixed-point manipulation of cervical spine still lacks relevant quantitative data. Moreover, the research on the influencing factors of cervical fixed-point rotation manipulation includes many parameters and there are differences, so it is necessary to further analyze its influencing factors to improve its related data.
    OBJECTIVE: To quantify the biomechanical parameters of cervical spine fixed-point rotation manipulation, explore the correlation between different biomechanical parameters, and the influence of individual characteristics of the subjects on the biomechanical parameters of cervical spine fixed-point rotation manipulation.
    METHODS: Totally 35 cases of cervical spondylosis were Outpatients from Orthopedic Department of Renai Branch of the First Affiliated Hospital of Guangxi University of Chinese Medicine and selected as the subjects investigated. Wearable mechanical measuring gloves were used to collect biomechanical parameters of cervical spine fixed-point rotation manipulation, including: thumb preload, thumb maximum thrust, palm preload, palm wrench force, and palm wrench maximum force. Personal characteristic parameters were collected, including age, height, weight, and neck circumference. The key biomechanical parameters in the process of cervical spine fixed-point rotation manipulation were analyzed and different individual characteristics were quantified. The results of biomechanical parameters were analyzed using Spearman correlation analysis. The possible effects of different individual characteristic parameters on biomechanics were analyzed.
    RESULTS AND CONCLUSION: (1) Compared with bilateral mechanical parameters, there was no significant difference between left manipulation and right manipulation (P > 0.05). (2) The average of thumb preload force was (7.21±1.19) N; the average of thumb maximum thrust was (28.40±4.48) N; the average of palm preload was (5.67±2.49) N; the average of palm wrench force was (10.90±5.11) N, and the average of palm wrench maximum force was (16.00±7.27) N. (3) There was a significant positive correlation between palm preload and palm wrench force (Rs=0.812, P < 0.01). There was a significant positive correlation between palm preload and palm wrench maximum force (Rs=0.773, P < 0.01). There was a significant positive correlation between palm wrench force and palm wrench maximum force (Rs=0.939, P < 0.01). (4) The weight was positively correlated with thumb preload, palm preload, palm wrench force and palm wrench maximum force (P < 0.05). (5) These findings confirm that there is a certain biomechanical standard value in the operation of cervical spine fixed-point rotation manipulation to treat cervical spondylosis. There is no significant difference between the left and right manipulations, which indicates that the manipulation has good consistency and repeatability. There is consistency and coordination among palm preload force, palm wrench force, and palm wrench maximum force. Their contributions to the therapeutic effect are similar. Body weight is an important factor affecting cervical spine fixed-point rotation manipulation.
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    Visualization analysis of artificial intelligence in bone trauma research based on Citespace
    Song Haoran, Zhang Yuqiang, Gu Na, Zhi Xiaodong, Wang Wei
    2025, 29 (3):  493-502.  doi: 10.12307/2025.129
    Abstract ( 265 )   PDF (2658KB) ( 681 )   Save
    BACKGROUND: The development of artificial intelligence in the medical field is rapidly advancing, with increasing research on its applications in the field of bone trauma. Through bibliometric analysis, this paper analyzed the research hotspots of artificial intelligence in the field of bone trauma in recent years, and predicted the future research trend.
    OBJECTIVE: To summarize the development history, research status, hot spots, and future development trends of artificial intelligence technology in the field of bone trauma to provide new insights for future research. 
    METHODS: This study selected relevant literature from the Web of Science core database, covering the period from the inception to August 2023, and retrieved 420 articles related to the application of artificial intelligence, machine learning, and deep learning in the field of bone trauma. After manual screening, 202 articles related to this article were exported, and Citespace software was used for visual analysis of cooperation of countries, institutions, cited journals, citation analysis, keyword co-occurrence, and other aspects.
    RESULTS AND CONCLUSION: (1) The overall number of publications from the 202 selected articles showed an upward trend, indicating significant research potential for future studies. The country with the highest centrality and the highest publication volume was the United States. The University of California (USA) was the most prolific research institution. (2) The top five most commonly used keywords in bone trauma research using artificial intelligence were deep learning, artificial intelligence, bone density, machine learning, and diagnosis. The keyword with the highest centrality was bone density, and the keyword with the highest frequency was deep learning. (3) The top 10 most cited reference papers provided comprehensive insights into the feasibility of applying artificial intelligence techniques to the diagnosis of bone trauma from various perspectives. Among them, eight papers focused on bone and joint injuries and deep convolutional neural networks. One paper discussed the use of deep learning in detecting osteoporosis in CT scans to prevent fragility fractures, while another paper explored the correlation between the application of artificial intelligence in identifying changes in skin texture and the recognition of bone characteristics. (4) In the future, the research hotspots of artificial intelligence will mainly focus on the specific study of fractures caused by bone and joint trauma and osteoporosis. The research trend mainly focuses on improving the performance of artificial intelligence algorithms, using new artificial intelligence technologies to accurately classify and quickly and efficiently diagnose bone injuries, especially for the diagnosis of complex and hidden fractures. By establishing finite element analysis models, more standardized evaluations of bone injuries can be achieved. 
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    Comparison of unicondylar knee arthroplasty and high tibial osteotomy in treatment of medial knee osteoarthritis
    Shi Lei, Shi Song, Lu Yue, Tao Ran, Ma Hongdong
    2025, 29 (3):  503-509.  doi: 10.12307/2025.121
    Abstract ( 122 )   PDF (1056KB) ( 240 )   Save
    BACKGROUND: The treatment of early knee osteoarthritis can be achieved through two knee preservation treatments: Unicondylar knee arthroplasty and high tibial osteotomy. However, further exploration is needed to determine whether there are differences in knee joint recovery between the two knee preservation surgeries at different stages after surgery. 
    OBJECTIVE: To compare the efficacy and related complications of unicondylar knee arthroplasty and high tibial osteotomy in the treatment of varus osteoarthropathy of the knee, and to provide a reference for clinical decision. 

    METHODS: A total of 103 patients with varus osteoarthritis of the knee underwent surgical treatment in the Affiliated Hospital of Nantong University from September 2018 to September 2022 were selected. Among them, 86 patients were followed up for more than 1 year. According to different surgical methods, the patients were divided into unicondylar knee arthroplasty group (49 cases) and high tibial osteotomy group (37 cases). Knee function, pain, and line of force correction were evaluated before surgery, 4 weeks, 3 months, 6 months, and 1 year after surgery in both groups. Hospital for special surgery knee score, functional score of Western Ontario and McMaster Universities Osteoarthritis Index, changes of lateral space of the knee joint, range of motion, proprioception (position sense), and postoperative activity recovery speed were evaluated comprehensively. 
    RESULTS AND CONCLUSION: (1) There were no significant differences in preoperative hospital for special surgery knee score, Western Ontario and McMaster Universities Osteoarthritis Index score and lateral knee compartment size between the two groups. (2) The hospital for special surgery knee score of patients undergoing unicondylar knee arthroplasty was better than that of patients undergoing high tibial osteotomy within 4 weeks after surgery (P < 0.05). At 3 and 6 months after surgery, compared with the improvement of the two groups, the hospital for special surgery knee score in the unicondylar knee arthroplasty group was lower than that in the high tibial osteotomy group, and the difference was significant (P < 0.05). The range of motion flexion value and position perception of patients undergoing high tibial osteotomy were significantly better than those undergoing unicondylar knee arthroplasty 6 months after surgery (P < 0.05). (3) The unicondylar knee arthroplasty group was better than the high tibial osteotomy group in terms of the speed of knee movement recovery (P < 0.05). (4) However, there was no significant difference between the two groups in the change of hospital for special surgery knee score, range of motion, and the width of lateral knee space during 1-year follow-up. (5) All patients were followed up for more than 1 year, and no adverse complications were found during the follow-up. (6) It is indicated that the short-term effect of knee functional recovery in patients with high tibial osteotomy is better than that in patients with unicondylar knee arthroplasty, but there is no significant difference in medium- and long-term efficacy between the two kinds of surgery for medial knee arthritis.
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    Design of asymmetric prosthesis and mechanical analysis of total knee arthroplasty
    Su Dejun, Dong Wanpeng, Dong Yuefu, Zhang Jichao, Zhang Zhen
    2025, 29 (3):  510-516.  doi: 10.12307/2025.122
    Abstract ( 118 )   PDF (1802KB) ( 100 )   Save
    BACKGROUND: Total knee arthroplasty serves as an effective intervention for the treatment of late-stage knee joint disorders. However, prosthetic liners are prone to wear and failure due to internal stress variations, resulting in limited lifespan and decreased postoperative patient activity. Addressing how to enhance prosthetic design to meet a broader range of patient needs constitutes a significant focus in prosthesis research.
    OBJECTIVE: Based on the morphological design of the meniscus, we propose an asymmetric design prosthesis and compare it with a symmetric posterior stabilized prosthesis. The stress distribution patterns and variations in the contact area of the liners for both prostheses were analyzed to explore whether the asymmetric prosthesis design offers advantages over the symmetric design.

    METHODS: Using the finite element method, we simulated the osteotomy and prosthesis assembly in a knee osteoarthritis patient. Two different prostheses (asymmetric design and posterior stabilized) were employed to establish post-total knee arthroplasty knee joint models. Under flexion conditions at 0°, 10°, 20°, and 30°, we investigated the Mises stress on the femoral and tibial components as well as the liner. Additionally, by comparing the contact area on the inner and outer sides of the liner, we aimed to explore the changes in biomechanics and alterations in motion behavior in the post-total knee arthroplasty knee joint.
    RESULTS AND CONCLUSION: (1) Throughout the flexion range from 0 to 30 degrees, the Mises stress peak on the liner exhibited a trend of initial decrease followed by an increase, with the stress on the medial side consistently surpassing that on the lateral side. (2) In comparison to the posterior stabilized prosthesis, the asymmetrically designed prosthesis demonstrated smaller stress peaks. At a flexion angle of 30 degrees, the Mises stress peak values of the medial and lateral parts of the asymmetric prosthesis were 15.81 MPa and 11.95 MPa, and those of the posterior stabilization prosthesis were 16.70 MPa and 13.76 MPa. The difference of Mises stress on the medial part was 5.33%, and the difference of Mises stress on the lateral part was 13.15%. Comparing the peak Mises stress on the femoral and tibial components, the asymmetric component was always lower than the posterior stable component during knee flexion. 
    (3) In the upright position at 0 degrees, the medial contact area of the posterior stabilization prosthesis was 17.96 mm2, and the lateral contact area was 
    34.10 mm2. The contact area on the inner and outer sides of the asymmetric design prosthesis liner was 105.47 mm2 and 107.80 mm2, respectively, indicating a larger contact area with a smaller difference between the inner and outer sides. (4) These results suggest that the biomechanical performance of the asymmetric prosthesis is superior, contributing to the maintenance of knee joint stability and improved joint mobility. This design, to a certain extent, mimics the rotational motion mechanism of the knee joint about the medial condyle as an axis, making it a more effective choice for knee joint prosthesis selection.
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    Comparison of decompression effects between spine endoscopy hybrid technique and uniportal endoscopic surgery in treatment of lumbar spinal stenosis with bilateral symptom
    Guo Song, Li Xinhua, Yan Meijun, Liu Yanbin, Liu Zhong, Li Kewei, Liu Pengcheng, Zhang Beiting, Fu Qiang
    2025, 29 (3):  517-523.  doi: 10.12307/2025.106
    Abstract ( 168 )   PDF (1402KB) ( 156 )   Save
    BACKGROUND: Spinal canal decompression using uniportal endoscopic surgery is a new minimally invasive surgery in the treatment of lumbar spinal stenosis. However, this technique needs a steep learning curve and high requirements for surgical equipment and instruments, which limits its clinical application. We previously use the spinal endoscopy as a monitoring endoscopy and combined with unilateral biportal endoscopy to propose a hybrid technique of spinal endoscopy to achieve coaxial endoscopic operation and hands-separate operation.
    OBJECTIVE: To compare the clinical outcome of hybrid technique and uniportal endoscopic surgery in treatment of lumbar spinal stenosis with bilateral lower limb pain symptoms. 
    METHODS: Ninety patients diagnosed of lumbar spinal stenosis with bilateral symptoms were included and retrospectively analyzed at First People’s Hospital, Shanghai Jiao Tong University from August 2020 to August 2022. 44 cases were included in group A (hybrid technique group), while 46 cases were included in group B (uniportal endoscopic surgery). The nerve decompression was observed during the surgery. Operation time, hospital stay time, and expenses were recorded in both groups. The visual analog scale scores of lower back pain and both lower extremities pain, Oswestry disability index scores of quality of life and excellent and good rate of modified Macnab criteria were recorded and compared at preoperative, postoperative 3 days, and postoperative 3 and 6 months. 
    RESULTS AND CONCLUSION: (1) The operation time of group A was significantly shorter than that of group B (P < 0.05). (2) The lower back pain and lower extremity pain of the severe side at postoperative 3 days, and 3 and 6 months were significantly relieved in both groups (P < 0.05). The visual analog scale score of lower extremity pain on the mild side was significantly decreased at postoperative 3 days, 3 and 6 months than preoperative score in the group A (P < 0.05). The visual analog scale score of lower extremity pain on the mild side was significantly decreased at postoperative 3 days than preoperative score in the group B (P < 0.05). The visual analog scale scores of lower extremity pain on the mild side at postoperative 3 and 6 months did not show significant difference than preoperative score in the group B. The comparison between the two groups showed that there was no significant difference in the visual analog scale scores of postoperative lower back pain and lower extremity pain of the severe side (P > 0.05). The visual analog scale scores of lower extremity pain on the mild side in the group A were significantly lower than those of group B at postoperative 3 and 6 months (P < 0.05). (3) The Oswestry disability index scores of both groups at postoperative 3 day were significantly lower than preoperative score (P < 0.05), and there was no significant difference between the two groups 3 days after operation. Oswestry disability index scores of group A at postoperative 3 and 6 months were significantly decreased than preoperative score (P < 0.05). The Oswestry disability index scores of group B at postoperative 3 and 6 months did not show significant differences than preoperative score (P > 0.05). The comparison between the two groups showed the Oswestry disability index scores of group A were significantly lower than group B at postoperative 3 and 6 months (P < 0.05). (4) The results of modified Macnab showed that the excellent and good rate of group A was significantly higher than that of group B (95%, 78%, P < 0.05). (5) It is indicated that the hybrid technique is a new spinal endoscopy technique, which has the advantages of less trauma and faster recovery as a minimally invasive surgery. The clinical outcome of hybrid technique is superior to that of uniportal endoscopic surgery in the treatment of lumbar spinal stenosis with bilateral symptoms. Additionally, it also has the advantages of good operational flexibility and high decompression efficiency as an open surgery. 
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    Fracture line map characteristics of distal radius fractures involving dorsal articular surface: effective fixation with screws for postoperative displacement
    Hu Liuchao, Luo Yiwen, Wu Zhifang
    2025, 29 (3):  524-530.  doi: 10.12307/2025.103
    Abstract ( 121 )   PDF (1428KB) ( 142 )   Save
    BACKGROUND: Palmar locking plate fixation is the most commonly used fixation method for distal radius fractures. However, when the fracture line involves the dorsal articular surface, palmar plate fixation carries a higher risk of postoperative displacement of the dorsal bone mass, especially the dorsal sigmoid notch bone mass.
    OBJECTIVE: To analyze the fracture line characteristics of distal radius fracture involving the dorsal articular surface, and further investigate the risk factors of postoperative displacement of the dorsal bone mass involving the sigmoid notch, so as to provide evidence for improving the success rate of surgery.
    METHODS: Retrospective analysis was performed on patients with distal radius fracture who were admitted to Third Affiliated Hospital of Guangzhou University of Chinese Medicine from January 2021 to September 2022. The number of dorsal fractures of the distal radius of 1, 2, 3 and above was divided into types I, II, and III based on preoperative CT images. The fracture line maps were drawn respectively to analyze the morphological characteristics of the dorsal fracture lines. Patients with dorsal sigmoid notch fracture were followed up for more than 3 months and divided into displaced group and non-displaced group according to whether the dorsal sigmoid notch bone mass displacement occurred after surgery. Age, sex, preoperative and postoperative CT anatomical parameters were compared between the two groups.
    RESULTS AND CONCLUSION: (1) 145 patients with type C of AO/OTA involving the dorsal articular surface were analyzed by fracture line map. According to the number of dorsal fractures, there were 25 cases of type I fracture (17.2%), 82 cases of type II fracture (56.6%), and 38 cases of type III fracture (26.2%). Fracture line map showed that the fracture line of type I fracture block mainly involved sigmoid notch; type II mainly involved sigmoid notch and lister tubercle, and type III involved sigmoid notch, lister tubercle, and dorsal radial column. Among the 145 patients, 86.2% (125/145 cases) were involved in sigmoid notch, of which type III was involved as high as 94.7% (36/38 cases); type II was involved as high as 88.0% (72/82 cases), and type I was involved as high as 68% (17/25 cases). (2) 76 cases of type C of AO/OTA involving the dorsal sigmoid notch were included for further study, of which 65 cases were not displaced after operation and 11 cases were displaced. In univariate analysis, there were no statistically significant differences between the two groups in age, sex, injury site, preoperative CT dorsal sigmoid notch bone mass length (d1), ulnar length (d2), dorsal height of dorsal sigmoid notch bone mass (d4), and ulnar edge distance between steel plate and radius (d5) (P > 0.05). The proportion of dorsal sigmoid notch involving the radioulnar joint: [d2/(d2+d3)], the proportion of articular surface of dorsal sigmoid notch (s1/s2), the distance between the ulnar screw tail and the edge of dorsal sigmoid notch (d6), and the number of screw fixation of dorsal sigmoid notch were statistically significant (P < 0.05). (3) Multivariate Logistic regression analysis showed that the number of screw fixation of sigmoid notch was the only risk factor affecting the displacement of sigmoid notch on dorsal ulnar side (P < 0.05). (4) It is indicated that type II is the most common intra-articular fracture of distal radius involving the back of the radius, followed by type III and type I with dorsal sigmoid notch bone mass. However, the dorsal sigmoid notch bone mass is prone to postoperative displacement due to the influence of the inferior radioulnar joint ligament, and the fixation of at least one effective screw during the operation can reduce the risk of displacement and help to improve the treatment effect.
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    Short-term efficacy of oblique lateral interbody fusion combined with lateral plate fixation in treatment of single-level lumbar degenerative disease
    Liu Xiaoyin, Zhang Jianqun, Chen Zhen, Liang Simin, Wang Zhiqiang, Ma Zongjun, Ma Rong, Ge Zhaohui
    2025, 29 (3):  531-537.  doi: 10.12307/2025.158
    Abstract ( 96 )   PDF (1281KB) ( 280 )   Save
    BACKGROUND: Stand-alone oblique lateral interbody fusion has a high rate of complications of fusion segment sink. Oblique lateral interbody fusion with posterior fixation can provide stable support, but intraoperative position changes and double incisions weaken the advantages of this technique. Oblique lateral interbody fusion combined with lateral plate fixation can achieve one-stage decompression in the same incision, while the lateral internal fixation provides stable support.

    OBJECTIVE: To analyze the short-term efficacy of oblique lateral interbody fusion combined with lateral plate fixation in the treatment of single-level lumbar degenerative disease. 

    METHODS: The clinical data of 34 patients with single-level lumbar degenerative disease treated with oblique lateral interbody fusion combined with lateral plate fixation were collected from May 2020 to October 2022. Among them, 14 were males and 20 were females aged from 41 to 72 years at the mean age of (58.6 ±9.9) years. There were 11 cases of lumbar spondylolisthesis (I°), 7 cases of lumbar disc herniation with segmental instability, and 16 cases of lumbar spinal stenosis. Operation time, blood loss, and complications were recorded. Visual analog scale scores of lumbago, radiative pain of both lower limbs, and Oswestry disability index scores were evaluated before surgery, 3 months after surgery, and the last follow-up. Dural sac cross-sectional area, intervertebral height, and intervertebral fusion were measured and observed. 
    RESULTS AND CONCLUSION: (1) The 34 patients were followed up for 14-36 months, with an average of (21.3±5.2) months. (2) The operation time ranged from 50 to 92 minutes, with an average of (68.5±11.1) minutes. Intraoperative blood loss was 50-170 mL, with an average of (71.6±25.3) mL. (3) Compared with the preoperative results, the visual analog scale scores and Oswestry disability index scores were significantly decreased at 3 months after surgery and at the last follow-up (P < 0.001), and the maximum Oswestry disability index scores were improved by nearly 50%. (4) Bone fusion was achieved in all patients during half-year follow-up. The overall complication rate was 21% (7/34), including 1 case of plate displacement, 3 cases of cage subsidence, 1 case of psoas weakness, and 2 cases of anterior thigh pain. (5) It is concluded that oblique lateral interbody fusion combined with lateral plate fixation for the treatment of lumbar degenerative diseases has the characteristics of less blood loss, short operation time, rapid postoperative recovery, and significant short-term clinical efficacy with the stable support to a certain extent. The long-term curative effect needs further follow-up observation. 
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    Repair strategies for nonunion in old osteoporotic vertebral compression fractures: a case analysis
    Zhao Xingcheng, Wang Jun, Lu Ming
    2025, 29 (3):  538-546.  doi: 10.12307/2025.132
    Abstract ( 107 )   PDF (1432KB) ( 192 )   Save
    BACKGROUND: Patients with osteoporotic compression fractures of the lumbar spine have varying degrees of changes in the sagittal parameters of the spine and pelvis, resulting in disequilibrium and rebalancing of the sagittal plane of the whole spine/lumbar spine. If the primary osteoporotic vertebral compression fracture is not treated promptly, it develops into an old osteoporotic vertebral compression fracture with nonunion over time.
    OBJECTIVE: To observe the effect of percutaneous vertebroplasty in nine patients with old osteoporotic lumbar compression fracture with nonunion. 
    METHODS: A retrospective analysis was conducted on the clinical data of nine elderly patients with old osteoporotic lumbar compression fractures with nonunion treated in the Orthopedics Department of Shanghai Public Health Clinical Center from September 2018 to August 2022. All patients underwent revision treatment with percutaneous vertebroplasty and received regular anti-osteoporosis treatment and rehabilitation training. The lumbar spine X-ray plain film and three-dimensional CT test results were reviewed before discharge and during follow-up to evaluate the diffusion of bone cement. Visual analog scale score, lumbar Oswestry disability index score, and Roussouly classification were recorded before, after surgery and during follow-up. 
    RESULTS AND CONCLUSION: (1) During postoperative follow-up, none of the 9 cases in this group experienced serious surgical complications such as nerve injury, infection, bone cement leakage, pulmonary embolism, and allergic shock, and no adverse reactions related to bone cement occurred. One patient died of respiratory failure due to COVID-19 infection 2 years after operation. (2) The visual analog scale score and Oswestry disability index score of postoperative pain in patients with recurrent vertebral fractures after percutaneous vertebroplasty were significantly lower than those before revision surgery. After the last follow-up percutaneous vertebroplasty revision surgery, the Roussouly classification of patients significantly improved. There was no statistically significant difference between the changes in vertebral body height during the last follow-up measurement and preoperative comparison. (3) These findings indicate that for patients with old osteoporotic lumbar compression fractures with nonunion, moderate injection of bone cement and effective dispersion of bone cement have a significant relieving effect on postoperative pain. Regular anti-osteoporosis and effective postoperative rehabilitation training can help patients improve their postoperative lumbar spine function.
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    Improvement of lower limb walking function in patients with chronic non-specific low back pain by biofeedback assisted electrical stimulation
    Zheng Xiang, Zhang Mingxing, Huang Ya, Shan Sharui
    2025, 29 (3):  547-553.  doi: 10.12307/2024.690
    Abstract ( 165 )   PDF (1341KB) ( 512 )   Save
    BACKGROUND: There have been many studies on biofeedback therapy and three-dimensional gait function analysis for chronic non-specific low back pain at home and abroad, but few studies on the combination of the two have been reported.
    OBJECTIVE: To study the effect of biofeedback assisted electrical stimulation on the change of walking ability in chronic non-specific low back pain by collecting gait parameter data through three-dimensional gait analysis.
    METHODS: Sixty patients with chronic non-specific low back pain, 34 males and 26 females, aged 32-58 years, were selected and admitted to First Affiliated Hospital of Guangdong Pharmaceutical University from June 2021 to September 2022. They were divided into control group (n=30) and trial group (n=30) according to the principle of random allocation. Both groups of patients received conventional treatment of dynamic interference with the waist, 20 minutes/time, once/day, 5 times a week, for 20 times. The control group received supine bypass and prone bypass suspension core muscle training on the basis of conventional treatment, 5 times a week, for 20 times. The trial group received biofeedback assisted electrical stimulation therapy on the basis of the control group, 20 minutes/ time, once/day, 5 times a week, for 20 times. The low back pain score, the mean value of electromyography and the gait parameters were compared and analyzed before and after treatment between the two groups.
    RESULTS AND CONCLUSION: (1) After treatment, the numerical rating scale score, Japanese Orthopaedic Association score, and Oswestry disability index of the two groups were significantly improved compared with those before treatment (P < 0.05). Numerical rating scale score, Japanese Orthopaedic Association score, and Oswestry disability index of the trial group were better than those of the control group (P < 0.05). (2) After treatment, the mean values of surface electromyography of rectus abdominis, gluteus maximus, and erector spinae muscle in the two groups were higher than those before treatment (P < 0.05), and the mean values of surface electromyography of rectus abdominis, gluteus maximus, and erector spinae muscle in the trial group were higher than those in the control group (P < 0.05). (3) After treatment, the step width, step speed, step frequency, step length ratio between healthy and diseased sides, the relative value of support between healthy and diseased sides, the relative value of swing between diseased and healthy sides, the sagittal motion range of hip joint and knee joint between healthy and diseased sides and the foot angle between healthy and diseased sides were all better than those before treatment in both groups (P < 0.05). The above indexes of the trial group were better than those of the control group after treatment (P < 0.05). (4) These results suggest that biofeedback assisted electrical stimulation can significantly alleviate chronic non-specific low back pain, and improve the lower limb walking function.
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    Ferroptosis and osteoporosis
    Yang Cheng, Li Weimin, Ran Dongcheng, Xu Jiamu, Wu Wangxiang, Xu Jiafu, Chen Jingjing, Jiang Guangfu, Wang Chunqing
    2025, 29 (3):  554-562.  doi: 10.12307/2025.134
    Abstract ( 237 )   PDF (1266KB) ( 247 )   Save
    BACKGROUND: It has also been confirmed that ferroptosis is closely related to a variety of musculoskeletal diseases, such as rheumatoid arthritis, osteosarcoma, and osteoporosis. The pathophysiological mechanisms of ferroptosis and osteoporosis need to be further studied and elucidated to broaden our understanding of iron metabolism and osteoporosis. It will provide research ideas for the future elucidation of new mechanisms of osteoporosis and the development of new technologies and drugs for the treatment of osteoporosis.
    OBJECTIVE: To provide an overview of the current status of research on ferroptosis in osteoporosis, to provide a new direction for future research on the specific molecular mechanisms of osteoporosis, and to provide more effective and better options for osteoporosis treatment strategies.
    METHODS: The first author used the computer to search the literature published from 2000 to 2024 in CNKI, WanFang, VIP, and PubMed databases with search terms “ferroptosis, iron metabolism, osteoporosis, osteoblast, osteoclast, bone metabolism, signal pathway, musculoskeletal, review” in Chinese and English. A total of 68 articles were finally included according to the selection criteria.
    RESULTS AND CONCLUSION: (1) Ferroptosis is a new type of cell death discovered in recent years, which is usually accompanied by a large amount of iron accumulation and lipid peroxidation during cell death, and its occurrence is iron-dependent. This is distinctly different from several types of cell death that are currently being hotly studied (e.g., cellular pyroptosis, necrotic apoptosis, cuproptosis, and autophagy). (2) Intracellular iron homeostasis is manifested as a balance between iron uptake, export, utilization, and storage. The body’s iron regulatory system includes systemic and intracellular regulation. The main factor of systemic regulation is hepcidin produced by hepatic secretion, and cellular regulation depends on the iron regulatory protein/iron response element system. Of course, intracellular iron homeostasis can be controlled by other factors, such as hypoxia, cytokines, and hormones. (3) Lipid peroxidation causes oxidative damage to biological membranes (plasma membrane and internal organelle membranes), lipoproteins, and other lipid-containing molecules. Polyunsaturated fatty acid-containing phospholipids are important targets of lipid peroxidation. Free polyunsaturated fatty acid is an important substrate for lipid oxidation and can bind to the phospholipid bilayer, leading to over-oxidation and thus triggering lipid apoptosis. (4) Several studies have shown that osteoblasts are overloaded with iron in different ways, resulting in the accumulation of unstable ferrous iron and the generation of reactive oxygen species and lipid peroxides, causing ferroptosis of osteoblasts and ultimately a decrease in bone formation, affecting bone homeostasis and the development of osteoporosis. (5) Osteoclasts are large multinucleated cells formed by the fusion of mononuclear macrophage cell lines or bone marrow mesenchymal stem cells induced by nuclear factor-κB ligand receptor activator, and they have the function of bone resorption. Iron ions can promote osteoclast differentiation and bone resorption through the production of intracellular lipid reactive oxygen species, while iron chelators can inhibit osteoclast formation in vitro and thus affect the occurrence and development of osteoporosis.
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    Regulation of osteogenic effects by bone morphogenetic protein/Wnt signaling pathway: revealing molecular mechanisms of bone formation and remodeling
    Liu Haowen, Qiao Weiping, Meng Zhicheng, Li Kaijie, Han Xuan, Shi Pengbo
    2025, 29 (3):  563-571.  doi: 10.12307/2024.697
    Abstract ( 214 )   PDF (1238KB) ( 180 )   Save
    BACKGROUND: Osteoblasts are the main cell types responsible for bone formation and remodeling, and the normal performance of their function is precisely regulated by various signaling pathways. Among them, the bone morphogenetic protein and Wnt signaling pathways play a key role in osteogenesis.
    OBJECTIVE: To review the role of bone morphogenetic protein/Wnt signaling pathway in the regulation of osteoblast function and analyze its changes in different physiological and pathological conditions in order to further reveal the molecular mechanism of bone formation and remodeling.
    METHODS: The Chinese and English search terms “BMP signaling pathway, Wnt signaling pathway, and osteogenesis” were searched in CNKI, Wanfang, and PubMed databases for original researches published from the inception to June 2023. Totally 61 articles were finally selected for analysis and summary. Using the method of the literature review, the studies of the bone morphogenetic protein/Wnt signaling pathway in regulating osteogenesis were sorted out and analyzed.
    RESULTS AND CONCLUSION: (1) Bone morphogenetic protein and Wnt signaling pathways play important roles in the differentiation, proliferation, and maturation of osteoblasts. Bone morphogenetic protein signaling pathway mainly regulates the expression of osteogenesis-related genes through the activation of Smad protein. Smad protein enters the nucleus and regulates the expression of genes related to osteogenesis. Different Wnt signaling pathway from bone morphogenetic protein mainly depends on the activation of β-catenin to exert its biological effects. (2) The regulatory effect of bone morphogenetic protein/Wnt signaling pathway will be affected by many factors in different physiological and pathological states. Growth factors, hormones, and mechanical stress can affect the activity of bone morphogenetic protein/Wnt signaling pathway to some extent. (3) Bone morphogenetic protein/Wnt signaling pathway interacts with other signaling pathways in the regulation of osteogenesis, and they together constitute a complex regulatory network. (4) Chinese medicine and natural compounds can promote bone health by regulating signaling pathways, providing new possibilities for treating bone diseases. (5) Future studies can further explore the interaction of bone morphogenetic protein/Wnt signaling pathway and other signaling pathways and its changes in different physiological and pathological conditions, resolve the key nodes and regulation mechanism in the complex network, to provide more precise targets for the treatment of bone-related diseases, and also provide new ideas to reveal the molecular mechanism of bone formation and remodeling.
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    Constructing a model of degenerative scoliosis using finite element method: biomechanical analysis in etiology and treatment
    He Kai, Xing Wenhua, Liu Shengxiang, Bai Xianming, Zhou Chen, Gao Xu, Qiao Yu, He Qiang, Gao Zhiyu, Guo Zhen, Bao Aruhan, Li Chade
    2025, 29 (3):  572-578.  doi: 10.12307/2025.116
    Abstract ( 151 )   PDF (941KB) ( 266 )   Save
    BACKGROUND: Degenerative scoliosis is defined as a condition that occurs in adulthood with a coronal cobb angle of the spine >10° accompanied by sagittal deformity and rotational subluxation, which often produces symptoms of spinal cord and nerve compression, such as lumbar pain, lower limb pain, numbness, weakness, and neurogenic claudication. The finite element method is a mechanical analysis technique for computer modelling, which can be used for spinal mechanics research by building digital models that can realistically restore the human spine model and design modifications.
    OBJECTIVE: To review the application of finite element method in the etiology and treatment of degenerative scoliosis.
    METHODS: The literature databases CNKI, PubMed, and Web of Science were searched for articles on the application of finite element method in degenerative scoliosis published before October 2023. Search terms were “finite element analysis, biomechanics, stress analysis, degenerative scoliosis, adult spinal deformity” in Chinese and English. Fifty-four papers were finally included.
    RESULTS AND CONCLUSION: (1) The biomechanical findings from the degenerative scoliosis model constructed using the finite element method were identical to those from the in vivo experimental studies, which proves that the finite element method has a high practical value in degenerative scoliosis. (2) The study of the etiology and treatment of degenerative scoliosis by the finite element method is conducive to the prevention of the occurrence of the scoliosis, slowing down the progress of the scoliosis, the development of a more appropriate treatment plan, the reduction of complications, and the promotion of the patients’ surgical operation. (3) The finite element method has gradually evolved from a single bony structure to the inclusion of soft tissues such as muscle ligaments, and the small sample content is increasingly unable to meet the research needs. (4) The finite element method has much room for exploration in degenerative scoliosis.
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    Mechanotransduction proteins in intervertebral disc degeneration
    Gao Xilin, Wu Si Zhang Chao Zhu Liguo, Fu Bifeng, Wang Ping
    2025, 29 (3):  579-589.  doi: 10.12307/2025.140
    Abstract ( 214 )   PDF (1306KB) ( 385 )   Save
    BACKGROUND: Recent research indicates that disc degeneration is closely related to abnormal stress load, and mechanotransduction proteins play a key role in it. 
    OBJECTIVE: To investigate the role and mechanism of mechanotransduction proteins in the mechanotransduction process induced by abnormal mechanical stimulation in disc degeneration, and to summarize the current treatment strategies targeting mechanotransduction to delay intervertebral disc degeneration.
    METHODS: Using “intervertebral disc, nucleus pulposus, annulus fibrosus, cartilaginous endplate, cell, mechanics, signal transduction, protein, biomechanics” as Chinese search terms, and “intervertebral disc, nucleus pulposus, annulus fibrosus, cartilaginous endplate, cell, mechanical stimulation, signal transduction, protein, biomechanics” as English search terms, relevant literature in the PubMed and CNKI databases was searched. A total of 88 articles were ultimately included for review. 
    RESULTS AND CONCLUSION: Disc cells can sense external mechanical stimulation through various mechanotransduction proteins and convert it into biological responses within the cells. These transduction proteins mainly include collagen proteins in the extracellular matrix, cell membrane surface receptors (such as integrins and ion channels), and cytoskeleton structural proteins. Their regulation of mechanotransduction processes primarily involves the activation of multiple pathways, such as the PI3K/AKT signaling pathway, nuclear factor-kB signaling pathway, and Ca2+/Calpain2/Caspase3 pathway. Mechanotransduction proteins play a key role in the mechanotransduction of disc cells. Abnormal expression of these proteins or resulting changes in the extracellular matrix environment can disrupt the mechanical balance of disc cells, leading to disc degeneration. In-depth study of the expression and regulatory mechanisms of mechanotransduction proteins in disc cells, and identification of key pathological links and therapeutic targets, is of significant importance for developing treatment strategies for disc degeneration. Current strategies to delay intervertebral disc degeneration by targeting mechanotransduction mainly include regulation of transduction proteins and improvement of the extracellular matrix. However, research in this area is still in its early stages. As research continues, new breakthroughs are expected in the regulation of disc degeneration by mechanotransduction proteins.
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    Traditional Chinese medicine monomer in treatment of neuroinflammation after spinal cord injury: effects of nuclear transcription factor kappa B signaling pathway
    Xu Zhenhua, Li Yanjie, Qin Hewei, Liu Haoyuan, Zhu Bochao, Wang Yupu
    2025, 29 (3):  590-598.  doi: 10.12307/2025.125
    Abstract ( 248 )   PDF (1200KB) ( 269 )   Save
    BACKGROUND: Targeted therapy based on nuclear transcription factor kappa B signaling pathway to explore neuroinflammation is increasingly worth exploring, and the advantages of Chinese medicine such as many targets, wide range, rich mechanisms, and few side effects have great potential in the treatment of various diseases.
    OBJECTIVE: Based on the nuclear transcription factor kappa B signaling pathway, this paper systematically expounded and summarized the research progress of kaempferol, safflower yellow, baicalin, and triptolide in the treatment of neuroinflammation after spinal cord injury. 
    METHODS: Search terms “spinal cord injury, inflammation, anti-inflammatory, traditional Chinese medicine monomer, monomeric compound, NF-κB signaling pathway, flavonoids, glycosides, phenols, esters, alkaloids” were searched in CNKI and PubMed databases. Totally 67 articles were finally included.
    RESULTS AND CONCLUSION: (1) The role of nuclear transcription factor kappa B signaling pathway in the nervous system is complex and diverse, which can regulate neutrophils, microglia, astrocytes, and macrophages, and mediate the occurrence and development of inflammation after injury. (2) The effects of traditional Chinese medicine monomers such as baicalin on the degradation of nuclear transcription factor kappa B inhibitory protein, the inhibition of phosphorylation process by safflowerin on nuclear transcription factor kappa B signaling pathway, and the inhibition of kaempferol on nuclear transcription factor kappa B signaling pathway p65 nuclear translocation can reduce the impact of inflammatory response on the body, thereby promoting the recovery of neurological function. (3) The nuclear transcription factor kappa B signaling pathway can promote inflammation and immune cell migration and activation in the early stage of injury, and can promote the repair of injury site and the occurrence of fibrosis in the middle and late stages of injury. Appropriate activation of the nuclear transcription factor kappa B signaling pathway can promote the release of inflammatory factors, improve the antioxidant capacity of cells, and promote the activation of immune cells, but the over-activated nuclear transcription factor kappa B signaling pathway can easily lead to the occurrence and continuation of chronic inflammation and the inhibition of apoptosis. (4) Future research can further explore how to accurately regulate the activation level of nuclear transcription factor kappa B signaling pathway, how to achieve precise intervention for nervous system inflammation and injury, and can also focus on the preparation of traditional Chinese medicine monomers and the mechanism of action of traditional Chinese medicine monomers on signaling pathways, in order to provide more effective treatment strategies for the rehabilitation and functional recovery of neurological diseases. 
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    A meta-analysis of clinical efficacy and safety of intravenous glucocorticoids before lower limb joint arthroplasty
    Wang Jianlei, He Peiliang, Sun Yongjian
    2025, 29 (3):  599-607.  doi: 10.12307/2025.118
    Abstract ( 164 )   PDF (2182KB) ( 79 )   Save
    OBJECTIVE: The clinical efficacy and safety of preoperative intravenous glucocorticoids in patients undergoing lower limb joint arthroplasty remain controversial. Therefore, we conducted a meta-analysis based on randomized controlled trials to evaluate the efficacy and safety of preoperative intravenous glucocorticoids for clinical treatment using lower limb joint arthroplasty. 
    METHODS: We systematically searched randomized controlled trials on the effects of preoperative intravenous glucocorticoids on the efficacy and safety of lower limb joint arthroplasty in Chinese and foreign databases up to June 2023, including PubMed, Embase, Web of Science, Cochrane Library, WanFang Data, and CNKI. The trial group was injected with intravenous glucocorticoid before operation to control pain. The control group received placebo or intravenous saline. Outcome measures included postoperative resting pain score, postoperative C-reactive protein, postoperative 5-m walking test pain score, length of hospital stay, operation time, postoperative morphine opioid supplemental dose, postoperative nausea and vomiting reaction, and postoperative periprosthesis infection complications.
    RESULTS: (1) A total of nine randomized controlled trials included 613 patients (n=311 in the glucocorticoid group, n=302 in the control group). (2) Compared with the control group, preoperative intravenous administration of glucocorticoids significantly reduced the resting pain scores of patients at 6 and 12 hours after surgery, as well as the pain scores of patients walking for 5 m on the first day after surgery. In addition, the need for additional morphine opioids and postoperative nausea and vomiting were reduced in the glucocorticoid group. The inflammatory C-reactive protein was lower than that of the control group within three days after surgery, and the length of hospital stay was shortened after intravenous glucocorticoid injection (P < 0.05). (3) However, there were no significant differences in the incidence of infection after arthroplasty, operation time, and pain scores at 24 and 48 hours between the two groups. 
    CONCLUSION: As an effective perioperative multi-modal analgesia protocol, intravenous injection of glucocorticoids before surgery is an effective and safe method to reduce hyperacute pain and improve joint mobility in patients with lower limb joint arthroplasty. More research is needed to determine the optimal dose and type of glucocorticoids for maximum pain control.
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    A network meta-analysis on therapeutic effect of different types of exercise on knee osteoarthritis patients
    Li Jia, Liu Qianru, Xing Mengnan, Chen Bo, Jiao Wei, Meng Zhaoxiang
    2025, 29 (3):  609-616.  doi: 10.12307/2025.130
    Abstract ( 248 )   PDF (3632KB) ( 160 )   Save
    OBJECTIVE: The main clinical manifestations of knee osteoarthritis are pain, swelling, stiffness, and limited activity, which have a serious impact on the life of patients. Exercise therapy can effectively improve the related symptoms of patients with knee osteoarthritis. This paper uses the method of network meta-analysis to compare the efficacy of different exercise types in the treatment of knee osteoarthritis.
    METHODS: CNKI, WanFang, PubMed, Embase, Cochrane Library, Web of Science, Scopus, Ebsco, SinoMed, and UpToDate were searched with Chinese search terms “knee osteoarthritis, exercise therapy” and English search terms “knee osteoarthritis, exercise”. Randomized controlled trials on the application of different exercise types in patients with knee osteoarthritis from October 2013 to October 2023 were collected. The outcome measures included visual analog scale, Western Ontario and McMaster Universities Osteoarthritis Index score, Timed Up and Go test, and 36-item short form health survey. Literature quality analysis was performed using the Cochrane Manual recommended tool for risk assessment of bias in randomized controlled trials. Two researchers independently completed the data collection, collation, extraction and analysis. RevMan 5.4 and Stata 18.0 software were used to analyze and plot the obtained data.
    RESULTS: A total of 29 articles with acceptable quality were included, involving 1 633 patients with knee osteoarthritis. The studies involved four types of exercise: aerobic training, strength training, flexibility/skill training, and mindfulness relaxation training. (1) The results of network meta-analysis showed that compared with routine care/health education, aerobic training could significantly improve pain symptoms (SMD=-3.26, 95%CI: -6.33 to -0.19, P < 0.05); strength training (SMD=-0.79, 95%CI: -1.34 to -0.23, P < 0.05) and mindfulness relaxation training (SMD=-0.79, 95%CI: -1.23 to -0.34, P < 0.05) could significantly improve the function of patients. Aerobic training (SMD=-1.37, 95%CI: -2.24 to -0.51, P < 0.05) and mindfulness relaxation training (SMD=-0.41, 95%CI: -0.80 to -0.02, P < 0.05) could significantly improve the functional mobility of patients. Mindfulness relaxation training (SMD=0.70, 95%CI: 0.21-1.18, P < 0.05) and strength training (SMD=0.42, 95%CI: 0.03-0.81, P < 0.05) could significantly improve the quality of life of patients. (2) The cumulative probability ranking results were as follows: pain: aerobic training (86.6%) > flexibility/skill training (60.1%) > strength training (56.8%) > mindfulness relaxation training (34.7%) > routine care/health education (11.7%); Knee function: strength training (73.7%) > mindfulness relaxation training (73.1%) > flexibility/skill training (56.1%) > aerobic training (39.9%) > usual care/health education (7.6%); Functional mobility: aerobic training (94.7%) > mindfulness relaxation training (65.5%) > strength training (45.1%) > flexibility/skill training (41.6%) > routine care/health education (3.2%); Quality of life: mindfulness relaxation training (91.3%) > strength training (68.0%) > flexibility/skill training (44.3%) > aerobic training (34.0%) > usual care/health education (12.3%).
    CONCLUSION: (1) Exercise therapy is effective in the treatment of knee osteoarthritis, among which aerobic training has the best effect on relieving pain and improving functional mobility. Strength training and mindfulness relaxation training has the best effect on improving patients’ function. Mindfulness relaxation training has the best effect on improving the quality of life of patients. (2) Limited by the quality and quantity of the included literature, more high-quality studies are needed to verify it.
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    Meta-analysis of anterior cervical decompression and fusion ROI-CTM self-locking system in treatment of degenerative cervical spondylosis
    Zhou Yanjie, Cao Chunfeng, Zhang Zhongzu, Niu Xiong, Wang Xin, Yang Zaihai, Zhou Liang, Li Bo
    2025, 29 (3):  617-627.  doi: 10.12307/2025.114
    Abstract ( 116 )   PDF (2199KB) ( 124 )   Save
    OBJECTIVE: Anterior cervical decompression and fusion is a classic surgical method for the treatment of degenerative cervical spondylosis. The use of nail plates increases the fusion rate and stability and indirectly leads to adjacent vertebral degeneration and postoperative dysphagia. In this paper, the clinical results and complications of ROI-CTM self-locking system and traditional cage combined with screw-plate internal fixation in the treatment of degenerative cervical spondylosis were compared by meta-analysis to provide evidence-based support for the selection of internal fixation methods in anterior cervical decompression and fusion.
    METHODS: CNKI, WanFang, VIP, PubMed, Cochrane Library, Web of Science, and Embase databases were searched for Chinese and English literature on the application of ROI-CTM self-locking system and fusion cage combined with screw plate internal fixation in the treatment of degenerative cervical spondylosis. The retrieval time range was from inception to July 2023. Two researchers selected the literature strictly according to the inclusion and exclusion criteria. The Cochrane bias risk tool was used to evaluate the quality of randomized controlled trials. Newcastle-Ottawa Scale was used to assess the quality of cohort studies. Meta-analysis was performed using RevMan 5.4 software. Outcome indicators included operation time, intraoperative blood loss, Japanese Orthopaedic Association score, Neck Disability Index, C2-C7 Cobb angle, fusion rate, incidence of adjacent vertebral degeneration, cage subsidence rate, and incidence of dysphagia.
    RESULTS: Thirteen articles were included, including eleven retrospective cohort studies and two randomized controlled trials, with 1 136 patients, 569 in the ROI-C group, and 567 in the cage combined with the nail plate group. Meta-analysis results showed that the operation time (MD=-15.52, 95%CI:-18.62 to -12.42, P < 0.000 01) and intraoperative blood loss (MD=-24.53, 95%CI:-32.46 to -16.61, P < 0.000 01) in the ROI-C group and the fusion device combined with nail plate group. Postoperative adjacent segment degeneration rate (RR=0.40, 95%CI:0.27-0.60, P < 0.000 01) and postoperative total dysphagia rate (RR=0.18, 95%CI:0.13-0.26), P < 0.000 01) were statistically different. The two groups had no significant difference in Japanese Orthopaedic Association score, Neck Disability Index, C2-C7 Cobb angle, fusion rate, or cage subsidence rate (P ≥ 0.05).
    CONCLUSION: Applying an ROI-CTM self-locking system and traditional cage combined with plate internal fixation in anterior cervical decompression and fusion can achieve satisfactory clinical results in treating degenerative cervical spondylosis. The operation of the ROI-CTM self-locking system is more straightforward. Compared with a cage combined with plate internal fixation, the ROI-CTM self-locking system can significantly reduce the operation time and intraoperative blood loss and has obvious advantages in reducing the incidence of postoperative dysphagia and adjacent segment degeneration. The ROI-CTM self-locking system is recommended for patients with skip cervical spondylosis and adjacent vertebral disease. However, given its possible high settlement rate, using a fusion cage combined with screw-plate internal fixation is still recommended for patients with degenerative cervical spondylosis with multiple segments and high-risk factors of fusion cage settlement, such as osteoporosis and vertebral endplate damage.
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    Visual analysis of hot spots and trends in the study of ligamentum flavum ossification
    Xu Qiang, Qin Jialin, Lian Zeshuang, Wang Aoting, Li Ding, Wang Ye, Wang Junfang
    2025, 29 (3):  628-636.  doi: 10.12307/2025.272
    Abstract ( 100 )   PDF (3171KB) ( 344 )   Save
    BACKGROUND: Ossification of the ligamentum flavum was previously considered to be rare in the population. As research has progressed, its incidence rate is increasing gradually, which has aroused the interest of a large number of researchers.
    OBJECTIVE: To visualize and analyze the research results on ossification of the ligamentum flavum from the Web of Science Core Collection since 1999 using bibliometric methods, and to review the research history of ossification of the ligamentum flavum, highlighting important literature, summarizing research hotspots, and providing ideas for researchers to find research directions.
    METHODS: Using the Web of Science Core Collection as the data source, relevant papers on ossification of the ligamentum flavum were searched and screened. VOSviewer 1.6.19 and CiteSpace 6.2.R6 were used to conduct the visual analysis of annual publication volume, research countries, institutions, citations, journals, authors, and keywords.
    RESULTS AND CONCLUSION: (1) A total of 347 papers were included. Since 1999, the number of published papers has increased in a spiral pattern. China’s research started later than Japan’s, but the number of publications has come up later, with Peking University being the institution with the most publications, and Prof. Chen Zhongqiang from Peking University being the scholar with the most publications. (2) Five of the 10 most frequently cited publications were related to the surgical treatment of the disease. (3) Excluding keywords directly related to the research topic and synthetically analyzing frequencies and betweenness centralities of key words, terms such as “thoracic myelopathy,” “dural ossification,” “minimally invasive surgery,” and “ossification of the posterior longitudinal ligament” occupied a central position in this field. (4) Keywords clustering analysis showed that clinical manifestations and surgical treatment of ossification of the ligamentum flavum accounted for a large proportion of study. (5) The timeline and burst analysis of keywords revealed that “minimally invasive surgery” appeared as a keyword around 2015, with the highest burst strength and the latest burst start time, and began to receive extensive attention from researchers in 2019. The burst of the keyword “dural ossification” has not yet ended. (6) Surgical treatment for ossification of the ligamentum flavum has been at the forefront of research. Development and research of minimally invasive surgery and research on dural ossification secondary to ossification of the ligamentum flavum are both current research hotspots and possible future research trends. 
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    Identification of core genes of osteoarthritis by bioinformatics
    Zhu Xuekun, Liu Heng, Feng Hui, Gao Yunlong, Wen Lei, Cai Xiaosong, Zhao Ben, Zhong Min
    2025, 29 (3):  637-644.  doi: 10.12307/2025.117
    Abstract ( 139 )   PDF (2333KB) ( 182 )   Save
    BACKGROUND: At present, osteoarthritis has become a major disease affecting the quality of life of the elderly, and the therapeutic effect is poor, often focusing on preventing the disease process, and the pathogenesis of osteoarthritis is still not fully understood. Bioinformatics analysis was carried out to explore the main pathogenesis of osteoarthritis and related mechanisms of gene coding regulation. 
    OBJECTIVE: To screen core differential genes with a major role in osteoarthritis by gene expression profiling. 
    METHODS: Datasets were downloaded from the Gene Expression Omnibus (GEO): GSE114007, GSE117999, and GSE129147. Differential genes in the GSE114007 and GSE117999 data collections were screened using R software, performing differential genes to weighted gene co-expression network analysis. The module genes most relevant to osteoarthritis were selected to perform protein interaction analysis. Candidate core genes were selected using the cytocape software. The candidate core genes were subsequently subjected to least absolute shrinkage and selection operator regression and COX analysis to identify the core genes with a key role in osteoarthritis. The accuracy of the core genes was validated using an external dataset, GSE129147. 
    RESULTS AND CONCLUSION: (1) A total of 477 differential genes were identified, 265 differential genes associated with osteoarthritis were obtained by weighted gene co-expression network analysis, and 8 candidate core genes were identified. The least absolute shrinkage and selection operator regression analysis finally yielded a differential gene ASPM with core value that was externally validated. (2) It is concluded that abnormal gene ASPM expression screened by bioinformatics plays a key central role in osteoarthritis. 
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    Visual analysis of treatment of adolescent idiopathic scoliosis
    Zheng Xiaodong, Gao Shan, Han Wenjin, Liu Lijun, Jia Menglong, Yu Longtan
    2025, 29 (3):  645-653.  doi: 10.12307/2025.137
    Abstract ( 253 )   PDF (3047KB) ( 195 )   Save
    BACKGROUND: At present, the incidence of scoliosis is increasing year by year, especially in adolescent idiopathic scoliosis. Therefore, it is more and more important to strengthen the research on the treatment of adolescent scoliosis.
    OBJECTIVE: To summarize the current status, hotspots, emerging trends, and frontiers of global research on the treatment of adolescent idiopathic scoliosis to provide reference and guidance for future related research. 
    METHODS: The literature related to the treatment of adolescent idiopathic scoliosis was retrieved on the Web of Science Core Collection (WOSCC) database from 2013 to 2023. CiteSpace 6.2.R1 software was used for visual analysis of countries, institutions, authors, and keywords. 
    RESULTS AND CONCLUSION: (1) A total of 561 English articles were included in this study. Among countries, institutions, and authors, the United States has contributed the most. Nanjing University and Qiu, Yong (Affiliated Drum Tower Hospital, Nanjing University School of Medicine) are the most published institution and author. The academic journal with the largest number of articles is the European Spine Journal. (2) In the analysis of cited literature, the top 10 most cited articles mainly describe the effects of surgical treatment and conservative treatment on improving adolescent idiopathic scoliosis, especially improving the curvature of patients. (3) Through the summary of highly cited articles and the keyword clustering, keyword prominence in-depth mining, the research hotspots are currently the relationship between Cobb angle and treatment choice, the therapeutic effect of exercise therapy and the therapeutic effect of posterior vertebral fusion. (4) The prognosis of patients with different curvatures has not been studied in depth, and the etiology of adolescent idiopathic scoliosis has not been clarified, so the relationship between curvature and prognosis and the etiology of adolescent idiopathic scoliosis may be a new research trend in the future. 
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    Mendelian randomization study on the association between telomere length and 10 common musculoskeletal diseases
    Luo Weidong, Pu Bin, Gu Peng, Huang Feng, Zheng Xiaohui, Chen Fuhong
    2025, 29 (3):  654-660.  doi: 10.12307/2025.221
    Abstract ( 133 )   PDF (1844KB) ( 141 )   Save
    BACKGROUND: Multiple observational studies have suggested a potential association between telomere length and musculoskeletal diseases. However, the underlying mechanisms remain unclear. 
    OBJECTIVE: To investigate the genetic causal relationship between telomere length and musculoskeletal diseases using two-sample Mendelian randomization analysis. 
    METHODS: Genome-wide association study summary data of telomere length were obtained from the UK Biobank. Genome-wide association study summary data of 10 common musculoskeletal diseases (osteonecrosis, osteomyelitis, osteoporosis, rheumatoid arthritis, low back pain, spinal stenosis, gout, scapulohumeral periarthritis, ankylosing spondylitis and deep venous thrombosis of lower limbs) were obtained from the FinnGen consortium. Inverse variance weighting, Mendelian randomization-Egger and weighted median methods were used to evaluate the causal relationship between telomere length and 10 musculoskeletal diseases. Inverse variance weighting was the primary Mendelian randomization analysis method, and sensitivity analysis was performed to explore the robustness of the results.
    RESULTS AND CONCLUSION: (1) Inverse variance-weighted results indicated a negative causal relationship between genetically predicted telomere length and rheumatoid arthritis (odds ratio=0.78, 95% confidence interval: 0.64-0.95, P=0.015) and osteonecrosis (odds ratio=0.56, 95% confidence interval: 0.36-0.90, P=0.016). No causal relationship was found between telomere length and the other eight musculoskeletal diseases (all P > 0.05). (2) Sensitivity analysis affirmed the robustness of these causal relationships, and Mendelian randomization-Egger intercept analysis found no evidence of potential horizontal pleiotropy (all P > 0.05). (3) This Mendelian randomized study supports that telomere length has protective effects against rheumatoid arthritis and osteonecrosis. However, more basic and clinical research will be needed to support our findings in the future.
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