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28 March 2019, Volume 23 Issue 在线 Previous Issue    Next Issue

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Research progress in preconditioning strategy promoting mesenchymal stem cell homing Wang Guo-ren, Bai Zhi-ming 2019, 23 (在线):  1-7.  doi: 10.3969/j.issn.2095-4344.2015.00.000 Abstract ( 452 )   Save Homing is the initial and key procedure of stem cells-based tissue restoration.Current studies have shown that the inability to recruit these mesenchymal stromal cells to target tissue with high efficiency remains a significant barrier to tissue restoration.Preconditioning strategy provides a new sight in promoting homing of stem cells.Altered local microenvironment via tissue preconditioning and enhanced expression of surface receptors or factors via cellular preconditioning are both capable of enhancing stem cell homing,thus facilitates restoration of impaired tissue.  Related Articles | Metrics

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Cloning and expression of β-glucanase gene from Bacillus subtilis in Escherichia coli Sun Ke, Wang Ya-nan, Zhang Ya-nan, Zhu Wen-hua, Cheng Dian-lin 2019, 23 (在线):  1-4.  Abstract ( 443 )   PDF (313KB) ( 649 )   Save BACKGROUND：β-glucan has been widely used for healthcare and clinical treatment. The molecular weight of the reasonable-sized glucan was obtained By the way of enzymatic hydrolysis which is more and more emphasized, but no research reports has been published in china yet. OBJECTIVE: The experiment aims to build an engineering strain secreting high-expression β-glucanase of which the activity and thermo-stability are analysed later so as to lay a basis on its clinical application. METHODS: We got the β-glucanase gene of Bacillus Subtilis，make β-glucanase gene and expression veltor pET28b(+) to recombinant plasmid using molecular biology technology and then structure engineered stain in Escherichia coli, using IPTG to induce the expression of the target potein, purificate it by nickel ion chelating resin. RESULTS AND CONCLUSION: In this research, Gene encoding β-glucanase in Bacillus subtilis was cloned into Escherichia coli and expressed efficiently. The enzymatic assay has found that it has great activity between 50°C and 60°C and highest in 50°C. The relative high-thermostability of β-glucanase under the temperature of 30°C~40°C. It was preliminarily found that β-glucanase expressed by the engineering strain referred was able to get rid of its substrate. But whether it is suitable for the clinical treatment requires more research. The experiment aims to utilize the strain to improve the level of clinical treatment and provide microbial resources. Keywords: Bacillus subtilis; Escherichia coli; plasmid; β-glucanase; clinical medicine; healthcare; cloning; expression; enzyme activity; treat Figures and Tables | References | Related Articles | Metrics

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Cardiac protection of prostacyclin secreted from endothelial progenitor cells against oxidative stress-induced apoptosis: verified by cardiac electrophysiological tests  2019, 23 (在线):  1-7.  doi: 10.3969/j.issn.2095-4344.2016.00.000 Abstract ( 488 )   PDF (1295KB) ( 382 )   Save BACKGROUND: Prostacyclin (PGI2) and its analogs have been reported to prevent pressure overload-induced cardiac hypertrophy, and to reduce cardiac ischemia/reperfusion injury. However, clinical application of PGI2 is challenging due to its short half-life (< 2 minutes). Thus, we have generated PGI2 expressing rat endothelial progenitor cell strains (PGI2-EPCs) that constitutively secrete prostacyclin. OBJECTIVE: To investigate the protective effect of PGI2-EPCs against oxidative stress-induced cardiomyocyte injury. METHODS: Cultured H9c2 cells in vitro were assigned into four groups: H9c2 cells treated by H2O2 for 4 hours. H9c2 cells were pretreated by conditioned medium (collected form EPCs and PGI2-EPCs or collected form EPCs and PGI2-EPCs mixed with native EPCs) before the addition of H2O2. PBS instead of conditioned mediums served as negative control. The paracrine effect of PGI2-EPCs on in vitro angiogenesis of native EPCs was evaluated. MTT and Hoechst 33342 assays were used to examine the protective effect of conditioned medium on H2O2-induced rat embryonic cardiomyocyte apoptosis and cell viability. Finally, we measured the effect of conditioned medium on the electric activities of adult cardiomyocytes by whole-cell patch clamp techniques. RESULTS AND CONCLUSION: When native EPCs mixed with conditioned medium of PGI2-EPCs, the total length of tubes was significantly longer compared with those mixed with CM of EPC. Rat embryonic cardiomyocytes pretreated with conditioned medium of PGI2-EPCs significantly reduced H2O2-induced apoptosis and preserved cell viability compared with pretreatment with EPC-conditioned medium and without pretreatment (P < 0.01). Pretreatment of rat adult cardiomyocytes with conditioned medium of PGI2-EPCs abolished H2O2-induced early afterdepolarization and shortened H2O2-induced action potential duration prolongation (P < 0.01) towards baseline. PGI2-EPCs protect against oxidative stress-induced cardiomyocyte injury through paracrine action. Our findings provide the groundwork for an innovative cell therapy approach to treat ischemic heart disease. Related Articles | Metrics

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The clinical effectiveness of single-level Isobar TTL dynamic internal fixation in the treatment of lumbar degenerative diseases Yixing Tian, Zhaohua Bao, Yiming Ji, Xin Mei, Jun Pan and Huilin Yang 2019, 23 (在线):  1-6.  Abstract ( 255 )   PDF (974KB) ( 296 )   Save BACKGROUND: The short-term effects of dynamic internal fixation and whether dynamic internal fixation system can prevent or delay the process of adjacent level degeneration after lumbar surgery remains in controversy. Objective: To investigate the short-term clinical effectiveness of single-level Isobar TTL dynamic internal fixation and in the treatment of degenerative lumbar diseases. Methods: From July 2014 to May 2016, 20 patients who suffered from lumbar degenerative diseases and underwent posterior decompression combined with single-level Isobar TTL dynamic internal fixation , including 11 male patients and 9 female patients, were retrospectively analyzed, the average age of these patients was 47.4. Visual analog scale(VAS), Japanese Orthopaedic Association scores(JOA) and Oswestry disability index(ODI) were used to assess clinical outcomes. Disc height, range of motion(ROM) , and University of California at Los Angeles Grading Scale(UCLA) were measured on X-ray films to evaluate the status of disc degeneration on both dynamic fixed segment(index segment) and superior adjacent segment. Results: During follow- up, all 20 surgeries were finished successfully without complications. 20 patients were followed from 6 to 27months, with an average of 10.2 months, all the patients showed significant improvements in VAS, JOA and ODI scores(P＜0.05). At the latest follow-up the ROM of index-segment appeared to be smaller than preoperative status. The disc height and UCLA classification on the index segment appeared to have no significant difference compared with those preoperatively. The latest follow-up data, including ROM, disc height and UCLA classification of superior adjacent segment appeared to have no significant difference compared with those preoperatively. Conclusion: Single-level Isobar TTL dynamic internal fixation proved to have satisfactory clinical outcomes when used in the treatment of lumbar degenerative diseases. During follow-up, no accelerated disc degeneration on either index segment or superior adjacent segment is observed. Related Articles | Metrics

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N-butylphthalide affects cognitive function of APP/PS1 transgenic mice (Alzheimer’s disease model)  Zhang Lin1, Liu Jinjie1, Zhao Yan2, Liu Yi1, Lin Jianwen1 2019, 23 (在线):  1-6.  doi: 10.3969/j.issn.2095-4344.1182 Abstract ( 597 )   PDF (874KB) ( 284 )   Save BACKGROUND: Amyloid-β protein is a key factor of pathogenesis of Alzheimer’s disease. N-butylphthalide, an anti-cerebral ischemia drug, has been shown to have therapeutic effects in vascular dementia. OBJECTIVE: To investigate whether N-butylphthalide treating APP/PS1 transgenic mice via the SPEP-ERK-CREB signaling pathway. METHODS: Thirty male APP/PS1 transgenic mice (mouse model of Alzheimer’s disease), SPF grade, aged 12 months were provided by Beijing Huafukang Biotechnology Co., Ltd., and 10 C57BL/6 wild-type mice, SPF grade, were provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. The animals were divided into four groups (n=10/group): C57BL/6 wild-type mice group, APP/PS1 group, and 10 and 30 mg/kg N-butylphthalide groups. The former two groups were given same volume of vegetable oil via gavage, for 17 consecutive days. The behavioral test was performed at 9-16 days. N-butylphthalide was given 40 minutes prior to Morris water maze task. The mice were killed at 17 days, and the proteins levels of STEP61, pERK1/2 and pCREB in brain cortex were detected by western blot assay. RESULTS AND CONCLUSION: (1) Consecutive 16-day 10 and 30 mg/kg N-butylphthalide could significantly improve spatial learning disability and working memory disorder of APP/PS1 transgenic mice in Morris water maze test. (2) N-butylphthalide partly reduced the level of activated STEP61, and inhibited the dephosphorylation of ERK and CREB. (3) These results suggest that N-butylphthalide protects against amyloid-β-induced neurodegeneration and cognitive decline in APP/PS1 transgenic mice, which may be potential for Alzheimer’s disease. Figures and Tables | References | Related Articles | Metrics

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Mechanism of Long-non-coding RNA-mediated Exercise Regulation of Bone tissue Autophagy   2019, 23 (在线):  11-17.  Abstract ( 250 )   PDF (742KB) ( 188 )   Save BACKGROUND: LncRNA has been a research hotspot in the field of biomedicine in recent years, and its mechanism of regulating the level of autophagy and the effect of exercise on it has yet to be revealed.  OBJECTIVE: To summarize the research progress of LncRNA in regulating the level of autophagy and the effect of exercise on it.  METHODS: Search for foreign language biomedical, biological, and sports journal systems such as CNKI database and PubMed. Chinese keywords: long-chain non-coding RNA, autophagy, bone metabolism, exercise; English keywords: Long non-coding RNA, autophagy, Bone tissue, Bone metabolism, Exercise.  RESULTS AND CONCLUSION: (1) Autophagy occurs in bone marrow mesenchymal stem cells(BMSCs), osteoblasts(OB), and osteoclasts(OC). (2) Exercise may inhibit the autophagy regulated by mTOR and post-activate the Akt/mTOR signal axis through AMPK, regulate OB or OC differentiation, and ultimately affect bone metabolism. (3) LncRNA can up-regulate or down-regulate under the influence of exercise. As a ceRNA, it negatively regulates downstream related miRNAs, and then inhibits downstream Akt/mTOR signaling pathway through AMPK/ULK1 or AMPK/PPARα signaling axis to initiate autophagy.  Figures and Tables | References | Related Articles | Metrics