Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3743-3746.doi: 10.3969/j.issn.1673-8225.2012.20.030

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Effects of serum containing Lycium barbarum polysaccharides on the intracellular Ca2+ concentration and the expression of collagen type Ⅰ in MC3T3-E1 cells in vitro  

Ma Feng, Gao Jun, Wang Yi-nong, Shen Jun, Ma Jing-zu   

  1. Department of Orthopedics, Ningxia People’s Hospital, Yinchuan  750021, Ningxia Hui Autonomous Region, China
  • Received:2011-09-18 Revised:2011-10-16 Online:2012-05-13 Published:2012-05-13
  • About author:Ma Feng★, Master, Attending physician, Department of Orthopedics, Ningxia People’s Hospital, Yinchuan 750021, Ningxia Hui Autonomous Region, China
  • Supported by:

     the Natural Science Foundation of Ningxia Hui Autonomous Region, No. NZ08140*

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Abstract:

BACKGROUND: Previous experiments have shown that Lycium barbarum polysaccharide has a remarkable therapeutic effect on osteoporosis in adult castrated female rats.
OBJECTIVE: To explore the effects of rat serum containing Lycium barbarum polysaccharides on the intracellular Ca2+ concentration and the expression of collagen type Ⅰ in osteoblastic MC3T3-E1 cells in vitro.
METHODS: The rat serum containing 5%, 10% and 20% Lycium barbarum polysaccharide was added into the media of osteoblastic MC3T3-E1 cells, respectively. Blank serum with 20% volume concentration was added into the control group. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability analysis was utilized to investigate the cell proliferation. The intracellular Ca2+ concentration was determined by laser scanning confocal microscope. The expression of collagen type Ⅰ was examined by immunocytochemical technique.
RESULTS AND CONCLUSION: Compared with control group, the rat serum containing 5% and 10% Lycium barbarum polysaccharides significantly promoted the proliferation of osteoblastic MC3T3-E1 cells (P < 0.05, P < 0.01). Rat serum containing 10% and 20% Lycium barbarum polysaccharides increased the intracellular Ca2+ concentration and the expression of collagen typeⅠin osteoblastic MC3T3-E1 cells (P < 0.05, P < 0.01). These findings indicate that rat serum containing Lycium barbarum polysaccharides may prevent and heal osteoporosis by affecting the intracellular Ca2+ concentration and the expression of collagen typeⅠ.

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