Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3703-3706.doi: 10.3969/j.issn.1673-8225.2012.20.021

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Effect of modified acidic fibroblast growth factor against cardiomyocyte apoptosis in neonatal rats after anoxia/reoxygenation

Li Yan1, Wang Yong-ling1, Li Xiao-kun2, Li Jing3   

  1. 1Department of Pathophysiology, Xinxiang Medical University, Xinxiang  453003, Henan Province, China; 2Institute of Biology and Natural Medicines, Wenzhou Medical College, Wenzhou  325027, Zhejiang Province, China; 3Department of Pathophysiology, Jinan University Medical School, Guangzhou  510632, Guangdong Province, China
  • Received:2012-02-03 Revised:2012-03-19 Online:2012-05-13 Published:2012-05-13
  • Contact: Li Jing, Associate researcher, Master’s supervisor, Department of Pathophysiology, Jinan University Medical School, Guangzhou 510632, Guangdong Province, China
  • About author:Li Yan★, Master, Associate professor, Department of Pathophysiology, Xinxiang Medical University Medical school, Xinxiang 453003, Henan Province, China drleeyan@163.com
  • Supported by:

     the National 863 Program of China, No. 2002AA223318*

Abstract:

BACKGROUND: Acidic fibroblast growth factor (aFGF) can protect cardiomyocytes from ischemia/reperfusion damage, but the proliferative ability of the cells induced by modified aFGF (maFGF) is decreased.
OBJECTIVE: To study the effect of aFGF and maFGF on cardiomytes apoptosis after anoxia/reoxygenation.
METHODS: Myocardial anoxia/reoxygenation models with primary cultured cardiomyocytes from neonatal SD rats were established and then treated with aFGF and maFGF. The apoptosis rate of cardiomyocytes was detected by flow cytometry assay and the survival rate of cardiomyocytes was measured by typan blue exclusion.
RESULTS AND CONCLUSION: Pretreatment with aFGF and maFGF decreased the apoptosis rate of cardiomyocytes after anoxia/reoxygenation (P < 0.01). It has been proved that aFGF and maFGF have notable effects on attenuating cardiomyocyte apoptosis after anoxia/reoxygenation.

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