Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (52): 9746-9749.doi: 10.3969/j.issn.1673-8225.2011.52.013

Previous Articles     Next Articles

Factors to influence the stability of the prosthesis after total hip replacement: Bone marrow mesenchymal stromal cells, bone morphogenetic protein and osteogenic potential

Li Xiao-miao1, Shen Yi1, Wang Wei-li1, Ren Wei-ping2   

  1. 1Department of Orthopedics, Renji Affiliated Hospital, Medical College of Shanghai Jiao Tong University, Shanghai  200127, China;  
    2Providence Hospital and Medical Centers, Southfield, MI and Department of Biomedical Engineering, Wayne State University, Detroit, MI
  • Received:2011-08-18 Revised:2011-10-28 Online:2011-12-24 Published:2011-12-24
  • Contact: Wang Wei-li, Chief physician, Professor, Department of Orthopedics, Renji Affiliated Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200127, China drwangwl@126.com
  • About author:Li Xiao-miao★, Master, Department of Orthopedics, Renji Affiliated Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200127, China leexm1123@hotmail.com
  • Supported by:

    Non-governmental International Scientific and Technological Cooperation Projects of Shanghai Science and Technology Committee, No. 09410706100*

PDF

322

Abstract:

BACKGROUND: The relationship between the osteogenic potential of the bone marrow microenvironment and long term stability after total hip replacement is not clear yet. The author makes a point, the fit intensity of the prosthesis can be predict through the osteogenic potential analysis of the bone marrow.
OBJECTIVE: To investigate the relationship between the activities of bone marrow mesenchymal stem cells, expression of osteogenic protein-signaling pathway related genes, osteogenic potential and the stability of the prosthesis.
METHODS: Bone marrow mesenchymal stem cells (BMSCs) were prepared from bone marrow aspirate obtained from the proximal femur metaphysis during total hip replacement (THR) surgery. Twelve patients were included in this study (8 men and 4 women, ages 50-84, body mass index 21.5-50). The isolated BMSCs were culture-expanded to passage one; non-confluent, undifferentiated cells were harvested for flow cytometry or for RNA isolation. Linear regression analysis was used to assess the correlation among indicators of osteogenic potential.
RESULTS AND CONCLUSION: The number of Stro-1+ cells, activity of alkaline phosphatase and expression of osteoblasts-related genes from BMSCs of these 12 patients varied significantly. There was a positive correlation between cells expressing the Stro-1 surface marker and bone morphogenetic protein receptor 1a, MSX2, Runx2 and the activity of alkaline phosphatase (P < 0.05), there was a significant correlation between the activity of alkaline phosphatase and the expression of Runx2 after the cells was cultured by osteoblats (P < 0.05). There was a highly diverse of the bone marrow microenvironment osteogenic potential among THR patients. The potential impact of the BMSCs profiles on the long term outcome of total joint replacement patients warrants further investigation.

CLC Number: