Abstract:

BACKGROUND: Nowadays, there are so many studies on manmade scaffold combined with cells for repair of bone defects. However, the restoration effects are not ideal because of the manmade scaffold lacking the nature structure of bone.
OBJECTIVE: To inoculate the bone marrow mesenchymal stem cells (BMSCs) in the deproteinized cancellous bone in order to repair segmental femoral defects of rats and evaluate the composite application prospect by restoration effects in vivo.
METHODS: BMSCs were isolated, cultured, proliferated and marked by BrdU in vitro. At the same time bovine deproteinized cancellous bone was prepared, and then combined with the marked cells in vitro. The bone defect models (5 mm in length) were created at the bilateral femur in rats and divided into three groups. The defects were implanted by BMSCs combined with deproteinized cancellous bone, deproteinized cancellous bone, and BMSCs respectively.
RESULTS AND CONCLUSION: The BrdU expressed in all cells of each group, and the expression was decreased as time went on. X-ray scores and hematoxylin-eosin stained histological scores showed that the osteogenic effect of the composite group was better than those of the other groups. Collagen protein-Ⅰ expression in the composite group was obviously increased as time went on and was stronger than the other groups. The osteogenesis ability of the composite of BMSCs and heterogeneous deproteinized cancellous bone is stronger than simple scaffolds. BMSCs have the osteogenesis ability, but they cannot repair segmental bone defects.