Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9397-9401.doi: 10.3969/j.issn.1673-8225.2011.50.022

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Effects of exogenous acidic fibroblast growth factor on the expression of p38MAPK and fibroblast growth-factor receptor 2 in rats with intestinal ischemia-reperfusion injury

Weng Li-xin1, Li Xiu-xia2, Fu Xiao-bing3   

  1. 1Department of Pathology, Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China
    2Department of Pathology, Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010059, Inner Mongolia Autonomous Region, China
    3Laboratory of Wound Repair, General Hospital of Chinese PLA, Beijing 100853, China
  • Received:2011-06-19 Revised:2011-09-26 Online:2011-12-10 Published:2011-12-10
  • Contact: Li Xiu-xia, Professor, Master’s supervisor, Department of Pathology, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China nmglxx@sina.com
  • About author:Weng Li-xin★, Master, Associate professor, Department of Pathology, Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China wenglixin2007@yahoo.cn
  • Supported by:

    the Key Program of the National Natural Science Foundation of China, No. 30230370*; the General Research Program of Education Bureau of Inner Mongolia Autonomous Region, No. NJZY07093*

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Abstract:

BACKGROUND: Different types of wound healing can be promoted by acidic fibroblast growth factors. It plays an important role in the repair of visceral injury.
OBJECTIVE: To explore the effect of exogenous acidic fibroblast growth factor on the expression of p38MAPK and fibroblast growth-factor receptor 2 in rats with intestinal ischemia-reperfusion injury.
METHODS: Rat superior mesenteric artery was microsurgical clipping for 45 minutes to construct intestinal ischemia-reperfusion injury model. Rats were treated with modified acidic fibroblast growth factor immediately after reperfusion. Rat small intestines samples were collected at the 2nd, 6th, 12th and 24th hours after reperfusion to examine.
RESULTS AND CONCLUSION: Fibroblast growth-factor receptors 2 were mainly distributed in epithelial cells of small intestinal villi at intestinal cavity, side wall and on the cell membrane of crypts toward cavity. There was no significant change in the expression of fibroblast growth-factor receptor 2 and p38MAPK in protein and mRNA level at the primary stage after reperfusion. Then the expression of fibroblast growth-factor receptor 2 and p38MAPK increased gradually along with the extension of time, and peaked at the 6th to 12th hours after reperfusion. The protein and mRNA expression of fibroblast growth-factor receptor 2 and p38MAPK in rat small intestines was further enhanced after treated with acidic fibroblast growth factor. These findings demonstrate that acidic fibroblast growth factor can promote the repair of intestinal ischemia-reperfusion injury by up-regulating the expression of fibroblast growth-factor receptor 2 and p38MAPK.

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